Abstract
Terminal 8mm sections from the third internode of dark grown 7 day old Pisum sativum cv Alaska seedlings were separated into membrane and soluble fractions. SDS gradient PAGE identified approximately 50 in vivo phosphorylated proteins and proved superior to 2-D SDS PAGE in terms of resolution and repeatability. Addition of indoleacetic acid (IAA), fusicoccin, or 2,4 dichlorophenoxyacetic acid to membranes resulted in no detectable change in the number or phosphorylation level of the labeled proteins during in vitro phosphorylation in the presence of submicromolar concentrations of calcium. Similar results were obtained with soluble proteins. In the absence of calcium, the level of in vitro protein phosphorylation was much less, but not auxin effects could be identified. Furthermore, treatment of the sections with IAA in vivo followed by cell fractionation and in vitro phosphorylation failed to identify auxin responsive proteins. Lastly, when sections were labeled with /sup 32/P inorganic phosphate in the presence of 17 uM IAA, no auxin specific changes were found in the level of phosphorylation or in the number of phosphorylated proteins. Auxin effects on phosphorylation are thus slight or below their detection limit.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Adding IAA, fusicoccin or 2,4-D to pea membranes caused no detectable change in the number or phosphorylation level of labelled proteins in vitro.
- Taxon
- pea
Lathyrus oleraceust0 - Material
- cv Alaskae0
- Organ / stage
- third internodee0
- Treatment
- indoleacetic acid (IAA), fusicoccin, or 2,4 dichlorophenoxyacetic acide1
- Outcome
- number or phosphorylation level of the labeled proteinse1
- Direction
- no detectable changee1
- Qualifier
- submicromolar concentrations of calciume1
- Setting
- in vitroe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Auxin effects on in vitro and in vivo protein phosphorylation in pea. [Pisum sativum]”
e0“Terminal 8mm sections from the third internode of dark grown 7 day old Pisum sativum cv Alaska seedlings were separated into membrane and soluble fractions”
e1“Addition of indoleacetic acid (IAA), fusicoccin, or 2,4 dichlorophenoxyacetic acid to membranes resulted in no detectable change in the number or phosphorylation level of the labeled proteins during in vitro phosphorylation in the presence of submicromolar concentrations of calcium”
Claim · model-written summary
The same auxin treatment caused no detectable change in phosphorylation of soluble pea proteins.
- Taxon
- peaLathyrus oleraceust0
- Material
- cv Alaskae0
- Organ / stage
- third internodee0
- Treatment
- indoleacetic acid (IAA), fusicoccin, or 2,4 dichlorophenoxyacetic acide1
- Outcome
- number or phosphorylation level of the labeled proteinse1
- Qualifier
- soluble proteinse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Auxin effects on in vitro and in vivo protein phosphorylation in pea. [Pisum sativum]”
e0“Terminal 8mm sections from the third internode of dark grown 7 day old Pisum sativum cv Alaska seedlings were separated into membrane and soluble fractions”
e1“Addition of indoleacetic acid (IAA), fusicoccin, or 2,4 dichlorophenoxyacetic acid to membranes resulted in no detectable change in the number or phosphorylation level of the labeled proteins during in vitro phosphorylation in the presence of submicromolar concentrations of calcium”
e2“Similar results were obtained with soluble proteins”
Claim · model-written summary
In the absence of calcium, in vitro protein phosphorylation was much lower, and no auxin effects could be identified.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- absence of calciume3
- Outcome
- level of in vitro protein phosphorylatione3
- Direction
- much lesse3
- Qualifier
- but not auxin effects could be identifiede3
- Setting
- in vitroe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Auxin effects on in vitro and in vivo protein phosphorylation in pea. [Pisum sativum]”
e3“In the absence of calcium, the level of in vitro protein phosphorylation was much less, but not auxin effects could be identified”
Claim · model-written summary
IAA applied in vivo followed by fractionation and in vitro phosphorylation failed to identify auxin-responsive proteins.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- treatment of the sections with IAA in vivoe4
- Outcome
- auxin responsive proteinse4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Auxin effects on in vitro and in vivo protein phosphorylation in pea. [Pisum sativum]”
e4“treatment of the sections with IAA in vivo followed by cell fractionation and in vitro phosphorylation failed to identify auxin responsive proteins”
Claim · model-written summary
17 uM IAA in vivo caused no auxin-specific change in phosphorylation level or in the number of phosphorylated proteins.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- 17 uM IAAe5
- Outcome
- level of phosphorylatione5
- Qualifier
- no auxin specific changese5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Auxin effects on in vitro and in vivo protein phosphorylation in pea. [Pisum sativum]”
e5“when sections were labeled with /sup 32/P inorganic phosphate in the presence of 17 uM IAA, no auxin specific changes were found in the level of phosphorylation or in the number of phosphorylated proteins”
Claim · model-written summary
Auxin effects on phosphorylation are slight or below the detection limit.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- Auxine6
- Outcome
- Auxin effects on phosphorylatione6
- Qualifier
- slight or below their detection limite6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Auxin effects on in vitro and in vivo protein phosphorylation in pea. [Pisum sativum]”
e6“Auxin effects on phosphorylation are thus slight or below their detection limit”
The same questions in other papers
indole-3-acetic acid · physiology: hormones · Lathyrus oleraceus (pea)Positive and negative results
1 in the opposite direction
- positive
indole-3-acetic acid · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →class · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.