Abstract
The function of PsBRC1, the pea (Pisum sativum) homolog of the maize (Zea mays) TEOSINTE BRANCHED1 and the Arabidopsis (Arabidopsis thaliana) BRANCHED1 (AtBRC1) genes, was investigated. The pea Psbrc1 mutant displays an increased shoot-branching phenotype, is able to synthesize strigolactone (SL), and does not respond to SL application. The level of pleiotropy of the SL-deficient ramosus1 (rms1) mutant is higher than in the Psbrc1 mutant, rms1 exhibiting a relatively dwarf phenotype and more extensive branching at upper nodes. The PsBRC1 gene is mostly expressed in the axillary bud and is transcriptionally up-regulated by direct application of the synthetic SL GR24 and down-regulated by the cytokinin (CK) 6-benzylaminopurine. The results suggest that PsBRC1 may have a role in integrating SL and CK signals and that SLs act directly within the bud to regulate its outgrowth. However, the Psbrc1 mutant responds to 6-benzylaminopurine application and decapitation by increasing axillary bud length, implicating a PsBRC1-independent component of the CK response in sustained bud growth. In contrast to other SL-related mutants, the Psbrc1 mutation does not cause a decrease in the CK zeatin riboside in the xylem sap or a strong increase in RMS1 transcript levels, suggesting that the RMS2-dependent feedback is not activated in this mutant. Surprisingly, the double rms1 Psbrc1 mutant displays a strong increase in numbers of branches at cotyledonary nodes, whereas branching at upper nodes is not significantly higher than the branching in rms1. This phenotype indicates a localized regulation of branching at these nodes specific to pea.
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Claims 14
Claim · model-written summary
Psbrc1 pea mutants have increased shoot branching.
- Taxon
- Pea
Lathyrus oleraceust0 - Material
- Psbrc1 mutante0
- Outcome
- shoot-branching phenotypee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pea Psbrc1 mutant displays an increased shoot-branching phenotype”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
Psbrc1 pea mutants synthesize strigolactone.
- Taxon
- PeaLathyrus oleraceust0
- Material
- Psbrc1 mutante0
- Outcome
- able to synthesize strigolactonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pea Psbrc1 mutant displays an increased shoot-branching phenotype, is able to synthesize strigolactone (SL)”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
Psbrc1 mutants do not respond to strigolactone application.
- Taxon
- PeaLathyrus oleraceust0
- Material
- Psbrc1 mutante0
- Treatment
- SL applicatione0
- Outcome
- does not responde0
- Direction
- does not responde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pea Psbrc1 mutant displays an increased shoot-branching phenotype, is able to synthesize strigolactone (SL), and does not respond to SL application.”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
rms1 mutants were relatively dwarf compared with Psbrc1 mutants.
- Taxon
- PeaLathyrus oleraceust0
- Material
- ramosus1 (rms1) mutante0
- Outcome
- dwarf phenotypee0
- Direction
- relatively dwarfe0
- Comparator
- Psbrc1 mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of pleiotropy of the SL-deficient ramosus1 (rms1) mutant is higher than in the Psbrc1 mutant, rms1 exhibiting a relatively dwarf phenotype”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
rms1 mutants showed more upper-node branching than Psbrc1 mutants.
- Taxon
- PeaLathyrus oleraceust0
- Material
- rms1e0
- Outcome
- branching at upper nodese0
- Direction
- more extensivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rms1 exhibiting a relatively dwarf phenotype and more extensive branching at upper nodes.”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
PsBRC1 is expressed predominantly in the axillary bud.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- axillary bude0
- Gene
- PsBRC1e0
- Outcome
- mostly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PsBRC1 gene is mostly expressed in the axillary bud”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
GR24 application upregulated PsBRC1 transcription.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- direct application of the synthetic SL GR24e0
- Outcome
- transcriptionally up-regulatede0
- Direction
- up-regulatede0
Not reportedGeneComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is transcriptionally up-regulated by direct application of the synthetic SL GR24”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
6-benzylaminopurine downregulated PsBRC1 transcription.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- 6-benzylaminopurinee0
- Outcome
- down-regulatede0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and down-regulated by the cytokinin (CK) 6-benzylaminopurine.”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
6-benzylaminopurine increased axillary bud length in Psbrc1 mutants.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- 6-benzylaminopurine applicatione0
- Outcome
- axillary bud lengthe0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Psbrc1 mutant responds to 6-benzylaminopurine application and decapitation by increasing axillary bud length”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
Decapitation increased axillary bud length in Psbrc1 mutants.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- decapitatione0
- Outcome
- axillary bud lengthe0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Psbrc1 mutant responds to 6-benzylaminopurine application and decapitation by increasing axillary bud length”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
Psbrc1 mutation did not lower xylem sap zeatin riboside.
- Taxon
- PeaLathyrus oleraceust0
- Material
- Psbrc1e0
- Outcome
- zeatin riboside in the xylem sape0
- Direction
- does not cause a decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Psbrc1 mutation does not cause a decrease in the CK zeatin riboside in the xylem sap”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
Psbrc1 mutation did not strongly increase RMS1 transcripts.
- Taxon
- PeaLathyrus oleraceust0
- Gene
- RMS1e0
- Outcome
- RMS1 transcript levelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“or a strong increase in RMS1 transcript levels”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
The rms1 Psbrc1 double mutant had strongly increased branch number at cotyledonary nodes.
- Taxon
- PeaLathyrus oleraceust0
- Material
- double rms1 Psbrc1 mutante0
- Outcome
- numbers of branches at cotyledonary nodese0
- Direction
- strong increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the double rms1 Psbrc1 mutant displays a strong increase in numbers of branches at cotyledonary nodes”
t0“The Pea TCP Transcription Factor”
Claim · model-written summary
Upper-node branching in the double mutant was not significantly higher than in rms1.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- branching at upper nodese0
- Direction
- not significantly highere0
- Comparator
- rms1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“branching at upper nodes is not significantly higher than the branching in rms1”
t0“The Pea TCP Transcription Factor”
The same questions in other papers
class · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- no effect
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.