Abstract
Plant cell wall composition is thought to reflect cellular responses to developmental and environmental signals. We have purified a 33 kDa protein from cell wall extracts of soybean seedlings which is most abundant in extracts from the hook region of the hypocotyl and is rich in proline and hydroxypyroline. In vivo {sup 3}H-proline labelling of hypocotyl tissues indicates that the hook tissue is the predominant site for synthesis of this protein. In unwounded hook, label is incorporated into a 33 kDa protein, while in wounded hook this and additional proteins rich in proline are synthesized. Similarly treated cell wall extracts analyzed by Western blot analysis, using a polyclonal antibody raised against this 33kD protein, showed that the 33 kDa protein is most abundant in cell wall extracts from the hook region of unwounded seedlings and does not increase upon wounding. An immunologically related 35kD protein is also apparent in extracts from wounded hooks and appears to co-migrate with one of the labelled proteins extractable from this tissue. These data indicate that there are two related, proline-rich cell wall proteins in the hook region of soybean seedlings, one of which (33 kDa) is prominent during seedling development and another (35 kDa)more » which is wound inducible.« less
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
A 33 kDa protein was purified from soybean seedling cell walls.
- Taxon
- soybean
Glycine maxe0 - Gene
- proteine0
- Outcome
- purified a 33 kDa proteine0
- Value
- 33 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“purified a 33 kDa protein from cell wall extracts of soybean seedlings”
Claim · model-written summary
The 33 kDa protein is most abundant in the hook region and rich in proline and hydroxypyroline.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- hook region of the hypocotyle0
- Outcome
- most abundant in extracts from the hook region of the hypocotyl and is rich in proline and hydroxypyrolinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most abundant in extracts from the hook region of the hypocotyl and is rich in proline and hydroxypyroline”
t0“soybean”
Claim · model-written summary
Hook tissue is the predominant site of protein synthesis.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- hook tissuee0
- Outcome
- predominant site for synthesis of this proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hook tissue is the predominant site for synthesis of this protein”
t0“soybean”
Claim · model-written summary
Wounded hooks synthesize additional proline-rich proteins.
- Taxon
- soybeanGlycine maxt0
- Treatment
- wounded hooke0
- Outcome
- additional proteins rich in proline are synthesizede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in wounded hook this and additional proteins rich in proline are synthesized”
t0“soybean”
Claim · model-written summary
The 33 kDa protein did not increase after wounding.
- Taxon
- soybeanGlycine maxt0
- Treatment
- woundinge0
- Outcome
- 33 kDa proteine0
- Direction
- does not increasee0
- Comparator
- unwounded seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“33 kDa protein is most abundant in cell wall extracts from the hook region of unwounded seedlings and does not increase upon wounding”
t0“soybean”
Claim · model-written summary
A related 35kD protein appeared in wounded hooks.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- wounded hookse0
- Outcome
- 35kD protein is also apparente0
- Value
- 35kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An immunologically related 35kD protein is also apparent in extracts from wounded hooks”
t0“soybean”
Claim · model-written summary
The 35kD protein appears to co-migrate with a labelled wound protein.
- Taxon
- soybeanGlycine maxt0
- Outcome
- co-migrate with one of the labelled proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“appears to co-migrate with one of the labelled proteins extractable from this tissue”
t0“soybean”
Claim · model-written summary
The hook has two related proline-rich proteins, one developmental and one wound inducible.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- hook regione0
- Outcome
- two related, proline-rich cell wall proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are two related, proline-rich cell wall proteins in the hook region of soybean seedlings, one of which (33 kDa) is prominent during seedling development and another (35 kDa)more » which is wound inducible”
The same questions in other papers
mechanical · quality: protein · Glycine max (soybean)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.