Abstract
White light strongly promotes dormancy in freshly harvested cereal grains, whereas dark and after-ripening have the opposite effect. We have analyzed the interaction of light and after-ripening on abscisic acid (ABA) and gibberellin (GA) metabolism genes and dormancy in barley (Hordeum vulgare 'Betzes'). Analysis of gene expression in imbibed barley grains shows that different ABA metabolism genes are targeted by white light and after-ripening. Of the genes examined, white light promotes the expression of an ABA biosynthetic gene, HvNCED1, in embryos. Consistent with this result, enzyme-linked immunosorbent assays show that dormant grains imbibed under white light have higher embryo ABA content than grains imbibed in the dark. After-ripening has no effect on expression of ABA biosynthesis genes, but promotes expression of an ABA catabolism gene (HvABA8'OH1), a GA biosynthetic gene (HvGA3ox2), and a GA catabolic gene (HvGA2ox3) following imbibition. Blue light mimics the effects of white light on germination, ABA levels, and expression of GA and ABA metabolism genes. Red and far-red light have no effect on germination, ABA levels, or HvNCED1. RNA interference experiments in transgenic barley plants support a role of HvABA8'OH1 in dormancy release. Reduced HvABA8'OH1 expression in transgenic HvABA8'OH1 RNAi grains results in higher levels of ABA and increased dormancy compared to nontransgenic grains.
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Claims 11
Claim · model-written summary
White light promotes HvNCED1 expression in barley embryos.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree1 - Organ / stage
- embryose0
- Treatment
- white lighte0
- Gene
- HvNCED1e0
- Outcome
- expression of an ABA biosynthetic genee0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“white light promotes the expression of an ABA biosynthetic gene, HvNCED1, in embryos”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
Under white light, dormant barley grains have higher embryo ABA than in darkness.
- Taxon
- barleyHordeum vulgaree1
- Organ / stage
- embryoe0
- Treatment
- white lighte0
- Outcome
- embryo ABA contente0
- Direction
- highere0
- Comparator
- darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dormant grains imbibed under white light have higher embryo ABA content than grains imbibed in the dark”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
After-ripening does not alter ABA biosynthesis gene expression.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- After-ripeninge0
- Outcome
- expression of ABA biosynthesis genese0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After-ripening has no effect on expression of ABA biosynthesis genes”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
After-ripening promotes HvABA8'OH1 expression after imbibition.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- After-ripeninge0
- Gene
- HvABA8'OH1e0
- Outcome
- expression of an ABA catabolism genee0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After-ripening has no effect on expression of ABA biosynthesis genes, but promotes expression of an ABA catabolism gene (HvABA8'OH1), a GA biosynthetic gene (HvGA3ox2), and a GA catabolic gene (HvGA2ox3) following imbibition”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
After-ripening promotes HvGA3ox2 expression after imbibition.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- After-ripeninge0
- Gene
- HvGA3ox2e0
- Outcome
- a GA biosynthetic genee0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After-ripening has no effect on expression of ABA biosynthesis genes, but promotes expression of an ABA catabolism gene (HvABA8'OH1), a GA biosynthetic gene (HvGA3ox2), and a GA catabolic gene (HvGA2ox3) following imbibition”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
After-ripening promotes HvGA2ox3 expression after imbibition.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- After-ripeninge0
- Gene
- HvGA2ox3e0
- Outcome
- a GA catabolic genee0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After-ripening has no effect on expression of ABA biosynthesis genes, but promotes expression of an ABA catabolism gene (HvABA8'OH1), a GA biosynthetic gene (HvGA3ox2), and a GA catabolic gene (HvGA2ox3) following imbibition”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
Blue light mimics white light effects on germination, ABA and hormone metabolism gene expression.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Blue lighte0
- Outcome
- germination, ABA levels, and expression of GA and ABA metabolism genese0
- Direction
- mimicse0
- Comparator
- white lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Blue light mimics the effects of white light on germination, ABA levels, and expression of GA and ABA metabolism genes.”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
Red light did not affect germination, ABA levels or HvNCED1.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Rede0
- Outcome
- germination, ABA levels, or HvNCED1e0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red and far-red light have no effect on germination, ABA levels, or HvNCED1.”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
Far-red light did not affect germination, ABA levels or HvNCED1.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- far-red lighte0
- Outcome
- germination, ABA levels, or HvNCED1e0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red and far-red light have no effect on germination, ABA levels, or HvNCED1.”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
HvABA8'OH1 RNAi grains had higher ABA than nontransgenic grains.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Reduced HvABA8'OH1 expressione0
- Gene
- HvABA8'OH1e0
- Outcome
- levels of ABAe0
- Direction
- highere0
- Comparator
- nontransgenic grainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced HvABA8'OH1 expression in transgenic HvABA8'OH1 RNAi grains results in higher levels of ABA and increased dormancy compared to nontransgenic grains.”
e1“barley (Hordeum vulgare 'Betzes')”
Claim · model-written summary
HvABA8'OH1 RNAi grains had increased dormancy over nontransgenic grains.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Reduced HvABA8'OH1 expressione0
- Gene
- HvABA8'OH1e0
- Outcome
- dormancye0
- Direction
- increasede0
- Comparator
- nontransgenic grainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced HvABA8'OH1 expression in transgenic HvABA8'OH1 RNAi grains results in higher levels of ABA and increased dormancy compared to nontransgenic grains.”
e1“barley (Hordeum vulgare 'Betzes')”
The same questions in other papers
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See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.