Paper · 2019 · BIOTROPIA

THE EFFECTIVENESS OF SOME ECTOMYCORRHIZAL FUNGI IN ALGINATE BEADS IN PROMOTING THE GROWTH OF SEVERAL DIPTEROCARP SEEDLINGS

SUPRIYANTO SUPRIYANTO

14
claims
21
verbatim quotes
5
citations
5
research areas
✓
Open access · cc-by-nc-nd

Abstract

The effectiveness of six species of ectomycorrhizal fungi (Scleroderma columnare, S.dictyosporum, Laccaria toccata, Rhizopogon luteolus, Amanita umbronata and Descomyces sp.) in alginate beads in promoting the growth of four species of dipterocarp seedlings (Shorea pinanga, S.leprosula, S.ovalis and Hopea odorata) were studied. Different sodium alginate concentrations of 5 g, 10 g, 15 g and 20 g/L were tested to find out the best bead's elasticity and spore germination. Seedling height, Relative Field Mycorrhizal Dependency, percentage of mycorrhizal colonization, nutrient absorption, and the formation of Hartig's net and mantle structure of dipterocarp seedlings were observed 4 months after inoculation. The best elasticity of alginate beads was found in the concentration of sodium alginate of 15 g/L. The best growth increment was found in Hopea odorata inoculated with Amanita umbronata (126.60 %) followed by Shorea pinanga inoculated with Descomyces sp. (27.10%), Shorea ovalis inoculated with Amanita umbronata (26.90 %) and Shorea leprosula inoculated with Descomyces sp. (24.20 %) over the control. The highest Relative Field Mycorrhizal Dependency was found in Hopea odorata followed by Shorea ovalis, S. pinanga and S. leprosula. The highest mycorrhizal colonization was obtained in Shorea pinanga inoculated with Descomyces sp. (75%), while inoculation with Amanita umbronata on S.leprosula, S.ovalis and Hopea odorata increased mycorrhizal colonization i.e. 64.5 %, 52.5%, and 46.2 %, respectively. Hartig's net and mantle structures were well formed in Shorea leprosula as well as S.ovalis seedlings with all mycorrhizal fungi tested, while in S.pinanga seedlings these structures were only well formed with Descomyces sp. There is no clear difference in P levels in the leaves following inoculation as compared to the controls. Key words: Mycorrhizas/Alginate beads/Scleroderma columnare/Scleroderma dictyosporumlLaccaria laccatalRhizopogon luteoluslAmanita umbronatalDescomyces sp./Growth/Dipterocarpace-ae/Seedlings.

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Claims 14

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Alginate beads had best elasticity at 15 g/L sodium alginate.

Taxon
dipterocarp seedlingst0
Treatment
sodium alginate of 15 g/Le0
Outcome
elasticity of alginate beadse0
Value
15 g/Le0

Verbatim

e0

“The best elasticity of alginate beads was found in the concentration of sodium alginate of 15 g/L.”

✓ located verbatim in the source · abstract, characters 714–812 · show in text ↑
t0

“DIPTEROCARP SEEDLINGS”

✓ located verbatim in the source · title, characters 101–122 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Hopea odorata inoculated with Amanita umbronata had a 126.60 % growth increment over control.

Taxon
Hopea odorataHopea odoratae0
Treatment
inoculated with Amanita umbronatae0
Interactor
Amanita umbronatae0symbiont
Outcome
growth incremente1
Direction
increaseinferrede0
Value
126.60 %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hopea odorata
Hopea odorata · TaxonSilviculture at Vietnamese Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Hopea odorata inoculated with Amanita umbronata (126.60 %) followed by Shorea pinanga”

✓ located verbatim in the source · abstract, characters 852–937 · show in text ↑
e1

“The best growth increment was found in Hopea odorata inoculated with Amanita umbronata (126.60 %) followed by Shorea pinanga inoculated with Descomyces sp. (27.10%), Shorea ovalis inoculated with Amanita umbronata (26.90 %) and Shorea leprosula inoculated with Descomyces sp. (24.20 %) over the control.”

✓ located verbatim in the source · abstract, characters 813–1116 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shorea pinanga inoculated with Descomyces sp. had 27.10% growth increment.

Taxon
Shorea pinangaRubroshorea pinangae0
Treatment
inoculated with Descomyces sp.e0
Interactor
Descomyces sp.Descomycese0symbiont
Outcome
growth incremente1
Direction
increaseinferrede0
Value
27.10%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Descomyces
Descomyces · InteractorJerry Cooper · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Shorea pinanga inoculated with Descomyces sp. (27.10%)”

✓ located verbatim in the source · abstract, characters 923–977 · show in text ↑
e1

“The best growth increment was found in Hopea odorata inoculated with Amanita umbronata (126.60 %) followed by Shorea pinanga inoculated with Descomyces sp. (27.10%), Shorea ovalis inoculated with Amanita umbronata (26.90 %) and Shorea leprosula inoculated with Descomyces sp. (24.20 %) over the control.”

✓ located verbatim in the source · abstract, characters 813–1116 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shorea ovalis inoculated with Amanita umbronata had 26.90 % growth increment.

Taxon
Shorea ovalisRubroshorea ovalise0
Treatment
inoculated with Amanita umbronatae0
Interactor
Amanita umbronatae0symbiont
Outcome
growth incremente1
Direction
increaseinferrede0
Value
26.90 %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rubroshorea ovalis
Rubroshorea ovalis · TaxonPatrice78500 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Shorea ovalis inoculated with Amanita umbronata (26.90 %)”

✓ located verbatim in the source · abstract, characters 979–1036 · show in text ↑
e1

“The best growth increment was found in Hopea odorata inoculated with Amanita umbronata (126.60 %) followed by Shorea pinanga inoculated with Descomyces sp. (27.10%), Shorea ovalis inoculated with Amanita umbronata (26.90 %) and Shorea leprosula inoculated with Descomyces sp. (24.20 %) over the control.”

✓ located verbatim in the source · abstract, characters 813–1116 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shorea leprosula inoculated with Descomyces sp. had 24.20 % growth increment over control.

Taxon
Shorea leprosulaRubroshorea leprosulae0
Treatment
inoculated with Descomyces sp.e0
Interactor
Descomyces sp.Descomycese0symbiont
Outcome
growth incremente1
Direction
increaseinferrede0
Value
24.20 %e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rubroshorea leprosula
Rubroshorea leprosula · TaxonPatrice78500 · Public domain · Wikimedia Commons ↗
Descomyces
Descomyces · InteractorJerry Cooper · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Shorea leprosula inoculated with Descomyces sp. (24.20 %) over the control.”

✓ located verbatim in the source · abstract, characters 1041–1116 · show in text ↑
e1

“The best growth increment was found in Hopea odorata inoculated with Amanita umbronata (126.60 %) followed by Shorea pinanga inoculated with Descomyces sp. (27.10%), Shorea ovalis inoculated with Amanita umbronata (26.90 %) and Shorea leprosula inoculated with Descomyces sp. (24.20 %) over the control.”

✓ located verbatim in the source · abstract, characters 813–1116 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Hopea odorata had the highest Relative Field Mycorrhizal Dependency.

Taxon
Hopea odorataHopea odoratae0
Outcome
Relative Field Mycorrhizal Dependencye0
Direction
higheste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hopea odorata
Hopea odorata · TaxonSilviculture at Vietnamese Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest Relative Field Mycorrhizal Dependency was found in Hopea odorata”

✓ located verbatim in the source · abstract, characters 1117–1193 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shorea pinanga inoculated with Descomyces sp. had 75% mycorrhizal colonization.

Taxon
Shorea pinangaRubroshorea pinangae0
Treatment
inoculated with Descomyces sp.e0
Interactor
Descomyces sp.Descomycese0symbiont
Outcome
mycorrhizal colonizatione1
Direction
higherinferrede0
Value
75%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Descomyces
Descomyces · InteractorJerry Cooper · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Shorea pinanga inoculated with Descomyces sp. (75%)”

✓ located verbatim in the source · abstract, characters 1303–1354 · show in text ↑
e1

“The highest mycorrhizal colonization was obtained in Shorea pinanga inoculated with Descomyces sp. (75%)”

✓ located verbatim in the source · abstract, characters 1250–1354 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Amanita umbronata inoculation increased mycorrhizal colonization of S.leprosula to 64.5 %.

Taxon
S.leprosulaRubroshorea leprosulae0
Treatment
inoculation with Amanita umbronatae0
Interactor
Amanita umbronatae0symbiont
Outcome
mycorrhizal colonizatione0
Direction
increasede0
Value
64.5 %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rubroshorea leprosula
Rubroshorea leprosula · TaxonPatrice78500 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“inoculation with Amanita umbronata on S.leprosula, S.ovalis and Hopea odorata increased mycorrhizal colonization i.e. 64.5 %, 52.5%, and 46.2 %”

✓ located verbatim in the source · abstract, characters 1362–1505 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Amanita umbronata inoculation increased mycorrhizal colonization of S.ovalis to 52.5%.

Treatment
inoculation with Amanita umbronatae0
Interactor
Amanita umbronatae0symbiont
Outcome
mycorrhizal colonizatione0
Direction
increasede0
Value
52.5%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rubroshorea ovalis
Rubroshorea ovalis · TaxonPatrice78500 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“inoculation with Amanita umbronata on S.leprosula, S.ovalis and Hopea odorata increased mycorrhizal colonization i.e. 64.5 %, 52.5%, and 46.2 %”

✓ located verbatim in the source · abstract, characters 1362–1505 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Amanita umbronata inoculation increased mycorrhizal colonization of Hopea odorata to 46.2 %.

Taxon
Hopea odorataHopea odoratae0
Treatment
inoculation with Amanita umbronatae0
Interactor
Amanita umbronatae0symbiont
Outcome
mycorrhizal colonizatione0
Direction
increasede0
Value
46.2 %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hopea odorata
Hopea odorata · TaxonSilviculture at Vietnamese Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“inoculation with Amanita umbronata on S.leprosula, S.ovalis and Hopea odorata increased mycorrhizal colonization i.e. 64.5 %, 52.5%, and 46.2 %”

✓ located verbatim in the source · abstract, characters 1362–1505 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Hartig net and mantle structures formed well in Shorea leprosula with all fungi tested.

Taxon
Shorea leprosulaRubroshorea leprosulae0
Outcome
Hartig's net and mantle structurese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rubroshorea leprosula
Rubroshorea leprosula · TaxonPatrice78500 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Hartig's net and mantle structures were well formed in Shorea leprosula as well as S.ovalis seedlings with all mycorrhizal fungi tested”

✓ located verbatim in the source · abstract, characters 1521–1656 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Hartig net and mantle structures formed well in S.ovalis with all fungi tested.

Outcome
Hartig's net and mantle structurese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rubroshorea ovalis
Rubroshorea ovalis · TaxonPatrice78500 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Hartig's net and mantle structures were well formed in Shorea leprosula as well as S.ovalis seedlings with all mycorrhizal fungi tested”

✓ located verbatim in the source · abstract, characters 1521–1656 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

In S.pinanga, Hartig net and mantle structures formed well only with Descomyces sp.

Taxon
S.pinangaRubroshorea pinangae0
Interactor
Descomyces sp.Descomycese0symbiont
Outcome
these structurese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Descomyces
Descomyces · InteractorJerry Cooper · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“in S.pinanga seedlings these structures were only well formed with Descomyces sp.”

✓ located verbatim in the source · abstract, characters 1664–1745 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Leaf P levels showed no clear difference after inoculation versus controls.

Taxon
dipterocarp seedlingst0
Treatment
inoculatione0
Outcome
P levels in the leavese0
Direction
no clear differencee0
Comparator
controlse0

Verbatim

e0

“There is no clear difference in P levels in the leaves following inoculation as compared to the controls.”

✓ located verbatim in the source · abstract, characters 1746–1851 · show in text ↑
t0

“DIPTEROCARP SEEDLINGS”

✓ located verbatim in the source · title, characters 101–122 · show in text ↑

inoculation · growth · Rubroshorea pinangaResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.