Abstract
Legumes can access atmospheric nitrogen through a symbiotic relationship with nitrogen-fixing bacteroids that reside in root nodules. In soybean, the products of fixation are the ureides allantoin and allantoic acid, which are also the dominant long-distance transport forms of nitrogen from nodules to the shoot. Movement of nitrogen assimilates out of the nodules occurs via the nodule vasculature; however, the molecular mechanisms for ureide export and the importance of nitrogen transport processes for nodule physiology have not been resolved. Here, we demonstrate the function of two soybean proteins - GmUPS1-1 (XP_003516366) and GmUPS1-2 (XP_003518768) - in allantoin and allantoic acid transport out of the nodule. Localization studies revealed the presence of both transporters in the plasma membrane, and expression in nodule cortex cells and vascular endodermis. Functional analysis in soybean showed that repression of GmUPS1-1 and GmUPS1-2 in nodules leads to an accumulation of ureides and decreased nitrogen partitioning to roots and shoot. It was further demonstrated that nodule development, nitrogen fixation and nodule metabolism were negatively affected in RNAi UPS1 plants. Together, we conclude that export of ureides from nodules is mediated by UPS1 proteins, and that activity of the transporters is not only essential for shoot nitrogen supply but also for nodule development and function.
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Claims 7
Claim · model-written summary
Soybean proteins GmUPS1-1 and GmUPS1-2 transport allantoin and allantoic acid out of the nodule.
- Taxon
- soybean
Glycine maxe0 - Gene
- GmUPS1-1 (XP_003516366) and GmUPS1-2 (XP_003518768)e0
- Outcome
- allantoin and allantoic acid transport out of the nodulee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean ureide transporters”
e0“Here, we demonstrate the function of two soybean proteins - GmUPS1-1 (XP_003516366) and GmUPS1-2 (XP_003518768) - in allantoin and allantoic acid transport out of the nodule”
Claim · model-written summary
Repression of GmUPS1-1 and GmUPS1-2 in nodules leads to an accumulation of ureides.
- Taxon
- soybeanGlycine maxe1
- Treatment
- repression of GmUPS1-1 and GmUPS1-2 in nodulese1
- Gene
- GmUPS1-1 and GmUPS1-2e1
- Outcome
- ureidese1
- Direction
- accumulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean ureide transporters”
e1“Functional analysis in soybean showed that repression of GmUPS1-1 and GmUPS1-2 in nodules leads to an accumulation of ureides and decreased nitrogen partitioning to roots and shoot”
Claim · model-written summary
Repression of GmUPS1-1 and GmUPS1-2 in nodules decreases nitrogen partitioning to roots and shoot.
- Taxon
- soybeanGlycine maxe1
- Treatment
- repression of GmUPS1-1 and GmUPS1-2 in nodulese1
- Outcome
- nitrogen partitioning to roots and shoote1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean ureide transporters”
e1“Functional analysis in soybean showed that repression of GmUPS1-1 and GmUPS1-2 in nodules leads to an accumulation of ureides and decreased nitrogen partitioning to roots and shoot”
Claim · model-written summary
In RNAi UPS1 plants, nodule development, nitrogen fixation and nodule metabolism are negatively affected.
- Taxon
- soybeanGlycine maxe1
- Treatment
- RNAi UPS1 plantse3
- Outcome
- nodule development, nitrogen fixation and nodule metabolisme3
- Direction
- negatively affectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean ureide transporters”
e1“Functional analysis in soybean showed that repression of GmUPS1-1 and GmUPS1-2 in nodules leads to an accumulation of ureides and decreased nitrogen partitioning to roots and shoot”
e3“It was further demonstrated that nodule development, nitrogen fixation and nodule metabolism were negatively affected in RNAi UPS1 plants”
Claim · model-written summary
Both transporters are present in the plasma membrane and expressed in nodule cortex cells and vascular endodermis.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- nodule cortex cells and vascular endodermise2
- Outcome
- presence of both transporters in the plasma membranee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean ureide transporters”
e1“Functional analysis in soybean showed that repression of GmUPS1-1 and GmUPS1-2 in nodules leads to an accumulation of ureides and decreased nitrogen partitioning to roots and shoot”
e2“Localization studies revealed the presence of both transporters in the plasma membrane, and expression in nodule cortex cells and vascular endodermis”
Claim · model-written summary
Export of ureides from nodules is mediated by UPS1 proteins.
- Taxon
- soybeanGlycine maxe1
- Gene
- UPS1 proteinse4
- Outcome
- export of ureides from nodulese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean ureide transporters”
e1“Functional analysis in soybean showed that repression of GmUPS1-1 and GmUPS1-2 in nodules leads to an accumulation of ureides and decreased nitrogen partitioning to roots and shoot”
e4“Together, we conclude that export of ureides from nodules is mediated by UPS1 proteins, and that activity of the transporters is not only essential for shoot nitrogen supply but also for nodule development and function”
Claim · model-written summary
Activity of the transporters is essential for shoot nitrogen supply and also for nodule development and function.
- Taxon
- soybeanGlycine maxe1
- Gene
- the transporterse4
- Outcome
- nodule development and functione4
- Qualifier
- essentiale4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean ureide transporters”
e1“Functional analysis in soybean showed that repression of GmUPS1-1 and GmUPS1-2 in nodules leads to an accumulation of ureides and decreased nitrogen partitioning to roots and shoot”
e4“Together, we conclude that export of ureides from nodules is mediated by UPS1 proteins, and that activity of the transporters is not only essential for shoot nitrogen supply but also for nodule development and function”