Abstract
Mass propagation via tissue culture technology could rescue threatened indigenous medicinal, pesticidal and timber plants. However, its application remains underutilized locally due to high costs of tissue culture consumables. Local efforts towards reduction of costs have centered on use of cheaper carbon sources such as table sugar, substitution of Murashige and Skoog and Gamborg’s salts with mixtures of fertilizers and substitution of conventional gelling agents agar, gelrite and phytogel with cassava starch. However, a gap exists on the identification of cheaper substitutes of conventional plant growth promoters yet these, particularly cytokinins, are important cost drivers in tissue culture. Half a gram of plant-cell-culture-tested Thidiazuron (TDZ) from Sigma-Aldrich costs 2,145 Euros (Ksh 235,500) while 1 g of Zeatin costs 4,220 Euros (Ksh 463,514). This study sought to determine whether a low-cost agrochemical TDZ that costed only 0.229 USD (Ksh 19.84) per gram and was recently reported by the present authors to support high frequency regeneration in mature zygotic embryos of M. volkensii, could elicit similar response in other explants. Cotyledons of mature seeds from wild trees in Kitui were cultured in ½ MS medium containing eight TDZ concentrations from 0 to 4 mg/l. High frequency (80 to 100 %) direct regeneration of shoots was observed within 14 days. TDZ treatments had a significant effect (Ftestp < 0.001) on the regeneration response in relation to the non-morphogenic controls. Shoots were elongated on ½ MS + 0.1 mg/l 6-Benzylaminopurine in combination with either 0.01 mg/l 1AA or 10% coconut water and rooted in ½ MS + 0.1 mg/l Indole-3-butyric acid. Low-cost agrochemical TDZ could substitute the exorbitant plant-cell-culture-tested Thidiazuron in tissue culture of M. volkensii. We recommend testing of this agrochemical on more explants and species.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Cotyledons showed 80 to 100 % direct shoot regeneration within 14 days.
Verbatim
e0“High frequency (80 to 100 %) direct regeneration of shoots was observed within 14 days.”
t0“MELIA VOLKENSII GURKE”
Claim · model-written summary
TDZ significantly affected regeneration relative to non-morphogenic controls.
- Taxon
- MELIA VOLKENSII GURKEt0
- Treatment
- TDZ treatmentse0
- Outcome
- regeneration responsee0
- Comparator
- non-morphogenic controlse0
Not reportedDirection
Verbatim
e0“TDZ treatments had a significant effect (Ftestp < 0.001) on the regeneration response in relation to the non-morphogenic controls.”
t0“MELIA VOLKENSII GURKE”
Claim · model-written summary
Shoots elongated under the stated benzylaminopurine combinations.
Verbatim
e0“Shoots were elongated on ½ MS + 0.1 mg/l 6-Benzylaminopurine in combination with either 0.01 mg/l 1AA or 10% coconut water”
t0“MELIA VOLKENSII GURKE”
Claim · model-written summary
Shoots rooted on indole-3-butyric acid medium.
Verbatim
e0“rooted in ½ MS + 0.1 mg/l Indole-3-butyric acid”
t0“MELIA VOLKENSII GURKE”
Claim · model-written summary
Authors concluded low-cost TDZ could substitute plant-cell-culture-tested thidiazuron.
Verbatim
e0“Low-cost agrochemical TDZ could substitute the exorbitant plant-cell-culture-tested Thidiazuron in tissue culture of M. volkensii.”
Claim · model-written summary
Authors recommend testing this agrochemical on more explants and species.
Verbatim
e0“We recommend testing of this agrochemical on more explants and species.”
t0“MELIA VOLKENSII GURKE”
The same questions in other papers
Thidiazuron · growth · Melia volkensiiResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.