Abstract
Adventitious shoot regeneration in woody species is regulated by interactions between plant growth regulators, endogenous hormone metabolism, and environmental cues such as light quality. Here, we investigated the effects of thidiazuron (TDZ) and the cytokinin oxidase/dehydrogenase (CKX) inhibitors INCYDE and phenyladenine (PA), in combination with different light spectra, on morphogenesis in Melia volkensii leaf explants. TDZ induced the highest frequencies of callus formation and adventitious shoot regeneration, particularly under white light. INCYDE promoted localized regeneration responses, including activation of dormant meristematic regions in secondary leaf axils, whereas PA showed limited regeneration efficiency. Light quality significantly influenced morphogenesis, with white and blue light favoring organized shoot development, while red and far-red light suppressed shoot regeneration and promoted callus formation. Cytokinin profiling revealed treatment-dependent shifts in endogenous cytokinin composition, most notably in isopentenyladenine (iP)-type cytokinins, which is consistent with altered cytokinin degradation dynamics. Cis-zeatin-type cytokinins were abundant across treatments, likely reflecting regulation associated with in vitro culture conditions. These findings indicate that cytokinin metabolism and light quality jointly influence organogenic competence in Melia volkensii Gürke, providing a physiological basis for optimizing regeneration strategies in woody plants. This study provides the first integrated analysis of cytokinin-modulating compounds and light spectra on adventitious shoot regeneration in Melia volkensii. The findings establish a physiological basis for improving regeneration protocols in recalcitrant woody species and support future biotechnological applications, including genetic improvement and advanced propagation strategies.
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Claims 8
Claim · model-written summary
TDZ produced the highest callus formation and adventitious shoot regeneration, especially in white light.
- Taxon
- Melia volkensiiMelia volkensiit0
- Treatment
- TDZe0
- Outcome
- callus formation and adventitious shoot regeneratione0
- Direction
- higheste0
- Qualifier
- white lighte0
Not reportedComparator
Verbatim
e0“TDZ induced the highest frequencies of callus formation and adventitious shoot regeneration, particularly under white light”
t0“Melia volkensii Gürke”
Claim · model-written summary
INCYDE promoted localized regeneration, including dormant meristem activation in secondary leaf axils.
- Taxon
- Melia volkensiiMelia volkensiit0
- Organ / stage
- secondary leaf axilse0
- Treatment
- INCYDEe0
- Outcome
- localized regeneration responsese0
- Direction
- promotede0
Not reportedComparator
Verbatim
e0“INCYDE promoted localized regeneration responses, including activation of dormant meristematic regions in secondary leaf axils”
t0“Melia volkensii Gürke”
Claim · model-written summary
PA showed limited regeneration efficiency.
- Taxon
- Melia volkensiiMelia volkensiit0
- Treatment
- PAe0
- Outcome
- limited regeneration efficiencye0
- Direction
- limitede0
Verbatim
e0“PA showed limited regeneration efficiency”
t0“Melia volkensii Gürke”
Claim · model-written summary
White and blue light favored organized shoot development.
- Taxon
- Melia volkensiiMelia volkensiit0
- Treatment
- white and blue lighte0
- Outcome
- organized shoot developmente0
- Direction
- favoringe0
Not reportedComparator
Verbatim
e0“white and blue light favoring organized shoot development”
t0“Melia volkensii Gürke”
Claim · model-written summary
Red and far-red light suppressed shoot regeneration.
- Taxon
- Melia volkensiiMelia volkensiit0
- Treatment
- red and far-red lighte0
- Outcome
- shoot regeneratione0
- Direction
- suppressede0
Not reportedComparator
Verbatim
e0“red and far-red light suppressed shoot regeneration and promoted callus formation”
t0“Melia volkensii Gürke”
Claim · model-written summary
Red and far-red light promoted callus formation.
- Taxon
- Melia volkensiiMelia volkensiit0
- Treatment
- red and far-red lighte0
- Outcome
- callus formatione0
- Direction
- promotede0
Not reportedComparator
Verbatim
e0“red and far-red light suppressed shoot regeneration and promoted callus formation”
t0“Melia volkensii Gürke”
Claim · model-written summary
Cytokinin treatments shifted endogenous cytokinin composition, notably iP-type cytokinins.
- Taxon
- Melia volkensiiMelia volkensiit0
- Outcome
- endogenous cytokinin compositione0
- Qualifier
- isopentenyladenine (iP)-type cytokininse0
Verbatim
e0“treatment-dependent shifts in endogenous cytokinin composition, most notably in isopentenyladenine (iP)-type cytokinins”
t0“Melia volkensii Gürke”
Claim · model-written summary
Cis-zeatin-type cytokinins were abundant across treatments.
- Taxon
- Melia volkensiiMelia volkensiit0
- Outcome
- Cis-zeatin-type cytokininse0
- Direction
- abundante0
Verbatim
e0“Cis-zeatin-type cytokinins were abundant across treatments”
t0“Melia volkensii Gürke”
The same questions in other papers
Thidiazuron · growth · Melia volkensiiResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.