Abstract
The latest studies outlined the important role of polyphenols from grapes and wines, especially from red variety. The polyphenols are those antioxidants compound who favouring the biochemical reactions which protect the cardiovascular system and also have an antiviral and bactericidal effect.[2] The aims of this paper was to determinate the variation of polyphenols content in different parts of grapes with spectrophotometry methods. One of the methods of determination is a spectral fingerprint UV-VIS (190-600 nm). The researches regarding the UV-VIS spectral fingerprints have been made on four red grape varieties: Merlot, Cabernet Sauvignon, Burgund mare and Muscat Hamburg harvested from two viticulture areas: West Carpathian Area (Timis - Recas) and South Carpathian Area (Dealu Mare-Merei). In the Viticulture laboratory of USAMV, the fresh grapes were separated in different mechanical parts (peels, seeds and cluster-rachis). After drying the components of grapes at 65oC, the samples were grounded and kept in a dry place. The samples used further for analysis was prepared in AGRIAL (Biomedical and Agroalimentary Expertise Laboratory) from USAMV Cluj-Napoca. It was used a Spectrophotometer Jasco V-530 for spectral fingerprint determination. For grape peels, seeds and clusters were taken 0.5 g from grounded sample and extracted for 20 minutes in 10 ml HCl 1% in methanol. The extract obtained was filtered and then was registered the UV-VIS spectrum in the wavelength domain between 190-600 nm [1]. For all samples some dilutions were made in order to obtain the spectrum in the absorption domain wanted. The spectra showed characteristic peaks of polyphenols (270-280 nm typical for phenolic acids and flavonoids and 535 nm specific to anthocyanins) found in higher quantities in wine grape varieties comparatively to table grape varieties, e.g. Burgund mare (Recas) and Cabernet Sauvignon (Recas) against Muscat Hamburg (Recas). For must samples, a larger number of maxima (270, 340, 380 nm) were observed without significant modifications between the grape varieties. For cluster samples it can be seen one characteristic signal for polyphenols from benzoic acid and flavon-3-ols in the wavelength domain between 270-280 nm. The samples from Merlot from Recas have the highest concentrations of these compounds and Merlot from Dealu Mare has the lowest value Seed sample analyzed show typical spectrum for polyphenols. Cabernet Sauvignon and Burgund Mare (Recas) have the highest concentrations of these compounds and to the other samples the values are lowest (but not significant). From quantitative point of view the polyphenols concentrations are bigger almost ten times in seeds. At the table grape variety Muscat Hamburg at all the samples the values was insignificant.
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Claims 7
Claim · model-written summary
Wine grape varieties showed higher characteristic polyphenol peaks than the table grape Muscat Hamburg.
Verbatim
e0“The spectra showed characteristic peaks of polyphenols (270-280 nm typical for phenolic acids and flavonoids and 535 nm specific to anthocyanins) found in higher quantities in wine grape varieties comparatively to table grape varieties, e.g. Burgund mare (Recas) and Cabernet Sauvignon (Recas) against Muscat Hamburg (Recas)”
Claim · model-written summary
In must samples, a larger number of maxima (270, 340, 380 nm) was observed, without significant differences between grape varieties.
Verbatim
e1“For must samples, a larger number of maxima (270, 340, 380 nm) were observed without significant modifications between the grape varieties”
Claim · model-written summary
In cluster samples one characteristic polyphenol signal from benzoic acid and flavon-3-ols appeared between 270 and 280 nm.
Verbatim
e0“The spectra showed characteristic peaks of polyphenols (270-280 nm typical for phenolic acids and flavonoids and 535 nm specific to anthocyanins) found in higher quantities in wine grape varieties comparatively to table grape varieties, e.g. Burgund mare (Recas) and Cabernet Sauvignon (Recas) against Muscat Hamburg (Recas)”
e2“For cluster samples it can be seen one characteristic signal for polyphenols from benzoic acid and flavon-3-ols in the wavelength domain between 270-280 nm”
Claim · model-written summary
Merlot from Recas had the highest polyphenol concentrations in cluster samples, above Merlot from Dealu Mare.
Verbatim
e0“The spectra showed characteristic peaks of polyphenols (270-280 nm typical for phenolic acids and flavonoids and 535 nm specific to anthocyanins) found in higher quantities in wine grape varieties comparatively to table grape varieties, e.g. Burgund mare (Recas) and Cabernet Sauvignon (Recas) against Muscat Hamburg (Recas)”
e3“The samples from Merlot from Recas have the highest concentrations of these compounds and Merlot from Dealu Mare has the lowest value”
Claim · model-written summary
Cabernet Sauvignon and Burgund Mare from Recas had the highest polyphenol concentrations in seed samples, compared with the other samples.
Verbatim
e0“The spectra showed characteristic peaks of polyphenols (270-280 nm typical for phenolic acids and flavonoids and 535 nm specific to anthocyanins) found in higher quantities in wine grape varieties comparatively to table grape varieties, e.g. Burgund mare (Recas) and Cabernet Sauvignon (Recas) against Muscat Hamburg (Recas)”
e4“Cabernet Sauvignon and Burgund Mare (Recas) have the highest concentrations of these compounds and to the other samples the values are lowest (but not significant)”
e5“Seed sample analyzed show typical spectrum for polyphenols”
Claim · model-written summary
Polyphenol concentrations in seeds were almost ten times higher than in the other parts.
Verbatim
e0“The spectra showed characteristic peaks of polyphenols (270-280 nm typical for phenolic acids and flavonoids and 535 nm specific to anthocyanins) found in higher quantities in wine grape varieties comparatively to table grape varieties, e.g. Burgund mare (Recas) and Cabernet Sauvignon (Recas) against Muscat Hamburg (Recas)”
e6“From quantitative point of view the polyphenols concentrations are bigger almost ten times in seeds”
Claim · model-written summary
Polyphenol values for the table grape Muscat Hamburg were insignificant in all samples.
Verbatim
e7“At the table grape variety Muscat Hamburg at all the samples the values was insignificant”