Abstract
Previously in this laboratory, studies indicated that photosynthesis (PS) of chloroplasts isolated from spinach plants which underwent osmotic adjustment during in situ water deficits was inhibited less at low osmotic potentials (Psi/sub s/) in vitro than PS of plastids isolated from well watered plants. In this study, an attempt was made to determine if chloroplast acclimation to low Psi/sub s/ was associated with in situ stromal solute accumulation. During a 14d stress cycle, in situ stromal volume was estimated by measuring (using the /sup 3/H/sub 2/O, /sup 14/C-sorbitol silicon oil centrifugation technique) the stromal space of plastids in solutions which had the Psi/sub s/ adjusted to the leaf Psi/sub s/. During the first lid of the cycle, stromal volume did not decline, despite a decrease of over 20% in the leaf RWC. After this time, stromal volume dropped rapidly. In situ stromal Psi/sub s/ was also estimated during a stress cycle. These studies indicated that stromal Psi/sub s/ was lowered by net solute accumulation. The data presented in this report suggest that chloroplast acclimation to low Psi/sub s/ may involve stromal solute accumulation and volume maintenance during cell water loss.
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Claims 4
Claim · model-written summary
Stromal volume remained stable initially despite a leaf relative water content decline over 20%.
- Taxon
- spinach
Spinacia oleraceae1 - Outcome
- stromal volumee0
- Direction
- did not declinee0
- Value
- over 20%e0
- Qualifier
- During the first lid of the cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During the first lid of the cycle, stromal volume did not decline, despite a decrease of over 20% in the leaf RWC.”
e1“spinach plants”
Claim · model-written summary
Stromal volume then fell rapidly.
- Taxon
- spinachSpinacia oleraceae1
- Outcome
- stromal volumee0
- Direction
- dropped rapidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After this time, stromal volume dropped rapidly.”
e1“spinach plants”
Claim · model-written summary
Stromal osmotic potential was lowered by net solute accumulation.
- Taxon
- spinachSpinacia oleraceae1
- Outcome
- stromal Psi/sub s/e0
- Direction
- lowerede0
- Qualifier
- net solute accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stromal Psi/sub s/ was lowered by net solute accumulation.”
e1“spinach plants”
Claim · model-written summary
The data suggest stromal solute accumulation and volume maintenance may contribute to chloroplast acclimation.
- Taxon
- spinachSpinacia oleraceae1
- Outcome
- chloroplast acclimatione0
- Qualifier
- stromal solute accumulation and volume maintenance during cell water losse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chloroplast acclimation to low Psi/sub s/ may involve stromal solute accumulation and volume maintenance during cell water loss.”
e1“spinach plants”