Abstract
Cold acclimation of Lolium L. and Zoysia Willd. Grass cultivars significantly increased regrowth of cryopreserved meristems. One wk of cold acclimation improved recovery following cryopreservation but extended acclimation (4-8 wk) resulted in the best regrowth. Cold acclimation also significantly increased the dehydration tolerance of both Zoysia and Lolium meristems. Lolium apices cold acclimated for 4 wk produced 60-100% regrowth following cryopreservation by slow freezing or encapsulation-dehydration. Cold-acclimated Zoysia had greater than 60% regrowth following encapsulation-dehydration when beads were dehydrated to less than 22% water content. Non-acclimated meristems of both genera had little or no regrowth. Thawed meristems grew quickly without callus formation and the plantlets produced were transplanted to pots in the greenhouse after 4 to 6 wk. Samples of each cultivar were stored in liquid nitrogen as part of the U.S. National Plant Germplasm System.
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Claims 10
Claim · model-written summary
Cold acclimation significantly increased regrowth of cryopreserved Lolium meristems.
- Taxon
- Lolium L.e0
- Treatment
- Cold acclimatione0
- Outcome
- regrowth of cryopreserved meristemse0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Cold acclimation of Lolium L. and Zoysia Willd. Grass cultivars significantly increased regrowth of cryopreserved meristems”
Claim · model-written summary
Cold acclimation significantly increased regrowth of cryopreserved Zoysia meristems.
- Taxon
- Zoysia Willd.e0
- Treatment
- Cold acclimatione0
- Outcome
- regrowth of cryopreserved meristemse0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Cold acclimation of Lolium L. and Zoysia Willd. Grass cultivars significantly increased regrowth of cryopreserved meristems”
Claim · model-written summary
One week of cold acclimation improved grass recovery following cryopreservation.
- Taxon
- grasst0
- Treatment
- One wk of cold acclimatione0
- Outcome
- recovery following cryopreservatione0
- Direction
- improvede0
Not reportedComparator
Verbatim
e0“One wk of cold acclimation improved recovery following cryopreservation”
t0“grass”
Claim · model-written summary
Extended cold acclimation of 4-8 weeks gave the best regrowth after cryopreservation.
Not reportedComparator
Verbatim
e0“extended acclimation (4-8 wk) resulted in the best regrowth”
t0“grass”
Claim · model-written summary
Cold acclimation significantly increased dehydration tolerance of Zoysia meristems.
ZoysiaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold acclimation also significantly increased the dehydration tolerance of both Zoysia and Lolium meristems”
Claim · model-written summary
Cold acclimation significantly increased dehydration tolerance of Lolium meristems.
LoliumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold acclimation also significantly increased the dehydration tolerance of both Zoysia and Lolium meristems”
Claim · model-written summary
Lolium apices cold acclimated for 4 weeks produced 60-100% regrowth after cryopreservation by slow freezing or encapsulation-dehydration.
- Organ / stage
- apicese0
- Treatment
- cold acclimated for 4 wke0
- Outcome
- regrowthe0
- Value
- 60-100%e0
- Qualifier
- slow freezing or encapsulation-dehydratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lolium apices cold acclimated for 4 wk produced 60-100% regrowth following cryopreservation by slow freezing or encapsulation-dehydration”
Claim · model-written summary
Cold-acclimated Zoysia had more than 60% regrowth after encapsulation-dehydration when beads were dehydrated to below 22% water content.
- Treatment
- encapsulation-dehydratione0
- Outcome
- regrowthe0
- Value
- greater than 60%e0
- Qualifier
- beads were dehydrated to less than 22% water contente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold-acclimated Zoysia had greater than 60% regrowth following encapsulation-dehydration when beads were dehydrated to less than 22% water content”
Claim · model-written summary
Non-acclimated meristems of both genera had little or no regrowth.
Verbatim
e0“Non-acclimated meristems of both genera had little or no regrowth”
t0“grass”
Claim · model-written summary
Thawed meristems grew quickly without callus formation.
Verbatim
e0“Thawed meristems grew quickly without callus formation”
t0“grass”
The same questions in other papers
cold · growth · LoliumPositive and negative results
1 in the opposite direction
- no effect
cold · growth · ZoysiaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.