Abstract
The plant vacuole is an important organelle for storing excess iron (Fe), though its contribution to increasing the Fe content in staple foods remains largely unexplored. In this study we report the isolation and functional characterization of two rice genes OsVIT1 and OsVIT2, orthologs of the Arabidopsis VIT1. Transient expression of OsVIT1:EGFP and OsVIT2:EGFP protein fusions revealed that OsVIT1 and OsVIT2 are localized to the vacuolar membrane. Ectopic expression of OsVIT1 and OsVIT2 partially rescued the Fe(2+) - and Zn(2+) -sensitive phenotypes in yeast mutant Δccc1 and Δzrc1, and further increased vacuolar Fe(2+) , Zn(2+) and Mn(2+) accumulation. These data together suggest that OsVIT1 and OsVIT2 function to transport Fe(2+) , Zn(2+) and Mn(2+) across the tonoplast into vacuoles in yeast. In rice, OsVIT1 and OsVIT2 are highly expressed in flag leaf blade and sheath, respectively, and in contrast to OsVIT1, OsVIT2 is highly responsive to Fe treatments. Interestingly, functional disruption of OsVIT1 and OsVIT2 leads to increased Fe/Zn accumulation in rice seeds and a corresponding decrease in the source organ flag leaves, indicating an enhanced Fe/Zn translocation between source and sink organs, which might represent a novel strategy to biofortify Fe/Zn in staple foods.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
OsVIT1 and OsVIT2 localize to the vacuolar membrane.
- Taxon
- rice
Oryza sativat0 - Gene
- OsVIT1 and OsVIT2e0
- Outcome
- localized to the vacuolar membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsVIT1 and OsVIT2 are localized to the vacuolar membrane”
t0“rice”
Claim · model-written summary
Ectopic OsVIT1 and OsVIT2 partially rescued Fe-sensitive yeast mutants.
- Taxon
- riceOryza sativat0
- Treatment
- Ectopic expression of OsVIT1 and OsVIT2e0
- Gene
- OsVIT1 and OsVIT2e0
- Outcome
- Fe(2+) - and Zn(2+) -sensitive phenotypese0
- Direction
- partially rescuede0
- Comparator
- yeast mutant Δccc1 and Δzrc1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of OsVIT1 and OsVIT2 partially rescued the Fe(2+) - and Zn(2+) -sensitive phenotypes in yeast mutant Δccc1 and Δzrc1”
t0“rice”
Claim · model-written summary
Ectopic expression increased vacuolar Fe, Zn and Mn accumulation in yeast.
- Taxon
- riceOryza sativat0
- Treatment
- OsVIT1 and OsVIT2e0
- Outcome
- vacuolar Fe(2+) , Zn(2+) and Mn(2+) accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of OsVIT1 and OsVIT2 partially rescued the Fe(2+) - and Zn(2+) -sensitive phenotypes in yeast mutant Δccc1 and Δzrc1, and further increased vacuolar Fe(2+) , Zn(2+) and Mn(2+) accumulation”
t0“rice”
Claim · model-written summary
OsVIT1 is highly expressed in flag leaf blade.
- Taxon
- riceOryza sativat0
- Organ / stage
- flag leaf bladee0
- Gene
- OsVIT1e0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsVIT1 and OsVIT2 are highly expressed in flag leaf blade and sheath, respectively”
t0“rice”
Claim · model-written summary
OsVIT2 is highly expressed in flag leaf sheath.
- Taxon
- riceOryza sativat0
- Organ / stage
- sheathe0
- Gene
- OsVIT2e0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsVIT1 and OsVIT2 are highly expressed in flag leaf blade and sheath, respectively”
t0“rice”
Claim · model-written summary
OsVIT2 responds strongly to Fe treatment.
- Taxon
- riceOryza sativat0
- Treatment
- Fe treatmentse0
- Gene
- OsVIT2e0
- Outcome
- responsivee0
- Direction
- highly responsivee0
- Comparator
- OsVIT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in contrast to OsVIT1, OsVIT2 is highly responsive to Fe treatments”
t0“rice”
Claim · model-written summary
Disrupting both transporters increased seed Fe/Zn accumulation.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedse0
- Treatment
- functional disruptione0
- Gene
- OsVIT1 and OsVIT2e0
- Outcome
- Fe/Zn accumulation in rice seedse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“functional disruption of OsVIT1 and OsVIT2 leads to increased Fe/Zn accumulation in rice seeds”
Claim · model-written summary
Disrupting both transporters decreased flag-leaf Fe/Zn accumulation.
- Taxon
- riceOryza sativae0
- Treatment
- functional disruption of OsVIT1 and OsVIT2e0
- Outcome
- source organ flag leavese0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“functional disruption of OsVIT1 and OsVIT2 leads to increased Fe/Zn accumulation in rice seeds and a corresponding decrease in the source organ flag leaves”
t0“rice”
The same questions in other papers
loss of function · nutrient: content · Oryza sativa (rice)Positive and negative results
19 papers in the same direction as this paper · 36 in the opposite direction
- negative
- positive
- positive
52 papers more in this group
See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.