Abstract
Iron (Fe) from rice grains is an important source of dietary intake; however, the molecular mechanisms responsible for loading of Fe to the grains are poorly understood. We functionally characterized a vacuolar iron transporter gene, OsVIT2 in terms of expression pattern, cellular localization, and mutant phenotypes. OsVIT2 was expressed in the parenchyma cell bridges of nodes, in the mestome sheath of leaf sheath and aleurone of the caryopsis. Mutation of OsVIT2 resulted in decreased Fe distribution to the leaf sheath, nodes, and aleurone, but increased Fe to the leaf blade and grains. Furthermore, Fe was heavily deposited in the parenchyma cell bridges, mestome sheath and aleurone in the wild-type rice, but this accumulation was decreased in the knockout lines. Conversely, heavier deposition of Fe was observed in the embryo and endosperm of the grains of knockout lines compared with the wild-type rice, resulting in increased Fe accumulation in the polished rice without yield penalty. These results indicate that OsVIT2 is involved in the distribution of Fe to the grains through sequestering Fe into vacuoles in mestome sheath, nodes, and aleurone layer and that knockout of this gene provides a potential way for Fe biofortification without yield penalty.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
OsVIT2 was expressed in node bridges, leaf sheath, and caryopsis aleurone.
- Taxon
- rice
Oryza sativat0 - Gene
- OsVIT2e0
- Outcome
- expressed in the parenchyma cell bridges of nodes, in the mestome sheath of leaf sheath and aleurone of the caryopsise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsVIT2 was expressed in the parenchyma cell bridges of nodes, in the mestome sheath of leaf sheath and aleurone of the caryopsis.”
t0“rice grains”
Claim · model-written summary
OsVIT2 mutation decreased iron distribution to leaf sheath, nodes, and aleurone.
- Taxon
- riceOryza sativat0
- Treatment
- Mutation of OsVIT2e0
- Gene
- OsVIT2e0
- Outcome
- Fe distribution to the leaf sheath, nodes, and aleuronee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of OsVIT2 resulted in decreased Fe distribution to the leaf sheath, nodes, and aleurone, but increased Fe to the leaf blade and grains.”
t0“rice grains”
Claim · model-written summary
OsVIT2 mutation increased iron in leaf blades and grains.
- Taxon
- riceOryza sativat0
- Treatment
- Mutation of OsVIT2e0
- Gene
- OsVIT2e0
- Outcome
- Fe to the leaf blade and grainse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of OsVIT2 resulted in decreased Fe distribution to the leaf sheath, nodes, and aleurone, but increased Fe to the leaf blade and grains.”
t0“rice grains”
Claim · model-written summary
Wild-type rice had heavy iron deposition in node bridges, mestome sheath, and aleurone.
- Taxon
- riceOryza sativae0
- Material
- wild-type ricee0
- Outcome
- Fe was heavily deposited in the parenchyma cell bridges, mestome sheath and aleuronee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fe was heavily deposited in the parenchyma cell bridges, mestome sheath and aleurone in the wild-type rice”
Claim · model-written summary
Knockout lines had decreased iron accumulation in those tissues.
- Taxon
- riceOryza sativae1
- Treatment
- knockout linese0
- Outcome
- accumulatione0
- Direction
- decreasede0
- Comparator
- wild-type ricee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this accumulation was decreased in the knockout lines”
e1“Fe was heavily deposited in the parenchyma cell bridges, mestome sheath and aleurone in the wild-type rice”
Claim · model-written summary
Knockout grains had heavier iron deposition in embryo and endosperm than wild type.
- Taxon
- riceOryza sativae0
- Organ / stage
- embryo and endosperme0
- Treatment
- knockout linese0
- Outcome
- deposition of Fee0
- Direction
- heaviere0
- Comparator
- wild-type ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heavier deposition of Fe was observed in the embryo and endosperm of the grains of knockout lines compared with the wild-type rice”
Claim · model-written summary
Knockout increased iron in polished rice without a yield penalty.
- Taxon
- riceOryza sativae0
- Treatment
- knockout linese1
- Outcome
- Fe accumulation in the polished ricee0
- Direction
- increasede0
- Qualifier
- without yield penaltye0
- Comparator
- wild-type ricee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased Fe accumulation in the polished rice without yield penalty”
e1“knockout lines compared with the wild-type rice”
Claim · model-written summary
OsVIT2 is interpreted to sequester iron into vacuoles in mestome sheath, nodes, and aleurone, governing grain distribution.
- Taxon
- riceOryza sativat0
- Gene
- OsVIT2e0
- Outcome
- distribution of Fe to the grainse0
- Qualifier
- sequestering Fe into vacuoles in mestome sheath, nodes, and aleurone layere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsVIT2 is involved in the distribution of Fe to the grains through sequestering Fe into vacuoles in mestome sheath, nodes, and aleurone layer”
t0“rice grains”
The same questions in other papers
loss of function · nutrient: content · Oryza sativa (rice)Positive and negative results
19 papers in the same direction as this paper · 36 in the opposite direction
- negative
- positive
- negative
52 papers more in this group
See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.