Abstract
The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied. The physiological data show that aminoguanidine (AG), an irreversible inhibitor of CuAO, inhibited adventitious rooting significantly. Among major catalysed and metabolised products or substrates of CuAO, only H2O2 obviously induced adventitious rooting and notably reversed the inhibitory effect of AG on adventitious rooting. The spatio-temporal change of CuAO activity in the rooting region of hypocotyls was histochemically measured using a peroxidase-coupled assay with the artificial substrate 3,3-diaminobenzidine tetrahydrochloride (DAB) as chromogen. DAB staining was detected in the parenchyma tissue between the vascular bundles, where roots originated before the emergence of root primordium, and then in the forming root primordium. With the origination of adventitious roots, the density and area of DAB staining increased rapidly. Most cells in root primodium had an evidently DAB staining, and strong staining mostly distributed in root meristem. H2O2, which was detected with the H2O2-sensitive probe 2,7-dichlorofluorescein diacetate, had a similar change to CuAO activity in this process. AG largely suppressed DAB staining and H2O2 fluorescence, and prevented root primordium formation. Taken together, these results clearly prove that, CuAO is responsible for H2O2 production during adventitious rooting, and H2O2 generated by CuAO is required for adventitious rooting in mung bean hypocotyls.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Aminoguanidine, an inhibitor of CuAO, significantly inhibited adventitious rooting in mung bean hypocotyls.
- Taxon
- mung bean
.jpg?width=60)
Vigna radiatae0 - Treatment
- aminoguanidine (AG)e1
- Gene
- CuAOe1
- Outcome
- adventitious rootinge1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
e1“The physiological data show that aminoguanidine (AG), an irreversible inhibitor of CuAO, inhibited adventitious rooting significantly”
Claim · model-written summary
H2O2 induced adventitious rooting in mung bean hypocotyls, unlike the other major CuAO products or substrates.
- Taxon
- mung beanVigna radiatae0
- Treatment
- H2O2e1
- Compound
- H2O2e1
- Outcome
- adventitious rootinge1
- Direction
- inducede1
- Comparator
- major catalysed and metabolised products or substrates of CuAOe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
e1“Among major catalysed and metabolised products or substrates of CuAO, only H2O2 obviously induced adventitious rooting and notably reversed the inhibitory effect of AG on adventitious rooting”
Claim · model-written summary
H2O2 reversed the inhibitory effect of aminoguanidine on adventitious rooting.
- Taxon
- mung beanVigna radiatae0
- Treatment
- H2O2e1
- Outcome
- inhibitory effect of AG on adventitious rootinge1
- Direction
- reversede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
e1“Among major catalysed and metabolised products or substrates of CuAO, only H2O2 obviously induced adventitious rooting and notably reversed the inhibitory effect of AG on adventitious rooting”
Claim · model-written summary
DAB staining indicating CuAO activity increased in the hypocotyl rooting region as adventitious roots originated.
- Taxon
- mung beanVigna radiatae0
- Organ / stage
- rooting region of hypocotylse1
- Gene
- CuAOe1
- Outcome
- density and area of DAB staininge2
- Direction
- increasede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
e1“The spatio-temporal change of CuAO activity in the rooting region of hypocotyls was histochemically measured”
e2“With the origination of adventitious roots, the density and area of DAB staining increased rapidly”
Claim · model-written summary
Aminoguanidine suppressed DAB staining and H2O2 fluorescence in mung bean hypocotyls.
- Taxon
- mung beanVigna radiatae0
- Treatment
- AGe1
- Outcome
- DAB staining and H2O2 fluorescencee1
- Direction
- suppressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
e1“AG largely suppressed DAB staining and H2O2 fluorescence, and prevented root primordium formation”
Claim · model-written summary
Aminoguanidine prevented root primordium formation in mung bean hypocotyls.
- Taxon
- mung beanVigna radiatae0
- Treatment
- AGe1
- Outcome
- root primordium formatione1
- Direction
- preventede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
e1“AG largely suppressed DAB staining and H2O2 fluorescence, and prevented root primordium formation”
Claim · model-written summary
H2O2 detected in mung bean hypocotyls changed similarly to CuAO activity during rooting.
- Taxon
- mung beanVigna radiatae0
- Outcome
- H2O2e1
- Direction
- similar changee1
- Comparator
- CuAO activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
e1“H2O2, which was detected with the H2O2-sensitive probe 2,7-dichlorofluorescein diacetate, had a similar change to CuAO activity in this process”
Claim · model-written summary
CuAO is responsible for H2O2 production during adventitious rooting, and H2O2 generated by CuAO is required for rooting in mung bean hypocotyls.
- Taxon
- mung beanVigna radiatae0
- Gene
- CuAOe1
- Compound
- H2O2e1
- Outcome
- H2O2 production during adventitious rootinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
e1“Taken together, these results clearly prove that, CuAO is responsible for H2O2 production during adventitious rooting, and H2O2 generated by CuAO is required for adventitious rooting in mung bean hypocotyls”
The same questions in other papers
hydrogen peroxide · growth · Vigna radiata (mung bean)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.