Abstract
A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1. During drought and salt stress, transformants (I5A) accumulated the methylated inositol D-ononitol in amounts exceeding 35 [mu]mol g-1 fresh weight In I5A, photosynthetic CO2 fixation was inhibited less during salt stress and drought, and the plants recovered faster than wild type. One day after rewatering drought-stressed plants, I5A photosynthesis had recovered 75% versus 57% recovery with cultivar SR1 plants. After 2.5 weeks of 250 mM NaCl in hydroponic solution, I5A fixed 4.9 [plus or minus] 1.4 [mu]mol CO2 m-2 s-1, whereas SR1 fixed 2.5 [plus or minus] 0.6 [mu]mol CO2 m-2 s-1. myo-Inositol, the substrate for IMT1, increases in tobacco under stress. Preconditioning of I5A plants in 50 mM NaCl increased D-ononitol amounts and resulted in increased protection when the plants were stressed subsequently with 150 mM NaCl. Pro, Suc, Fru, and Glc showed substantial diurnal fluctuations in amounts, but D-ononitol did not. Plant transformation resulting in stress-inducible, stable solute accumulation appears to provide better protection under drought and salt-stress conditions than strategies using osmotic adjustment by metabolites that are constitutively present.
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Claims 10
Claim · model-written summary
Transformant I5A accumulated D-ononitol in amounts exceeding 35 umol g-1 fresh weight during drought and salt stress.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume1 - Material
- I5Ae2
- Treatment
- drought and salt stresse2
- Gene
- IMT1e1
- Outcome
- D-ononitole2
- Direction
- accumulatede2
- Value
- 35 [mu]mol g-1 fresh weighte2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e2“During drought and salt stress, transformants (I5A) accumulated the methylated inositol D-ononitol in amounts exceeding 35 [mu]mol g-1 fresh weight”
Claim · model-written summary
In I5A tobacco, photosynthetic CO2 fixation was inhibited less during salt stress and drought than in wild type.
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Material
- I5Ae2
- Treatment
- salt stress and droughte2
- Outcome
- photosynthetic CO2 fixatione2
- Direction
- inhibited lesse2
- Comparator
- wild typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e2“In I5A, photosynthetic CO2 fixation was inhibited less during salt stress and drought, and the plants recovered faster than wild type”
Claim · model-written summary
One day after rewatering, I5A photosynthesis recovered to 75% against 57% recovery in cultivar SR1.
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Material
- I5Ae2
- Treatment
- rewatering drought-stressed plantse2
- Outcome
- photosynthesise2
- Direction
- recoverede2
- Value
- 75%e2
- Qualifier
- 57% recoverye2
- Comparator
- cultivar SR1 plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e2“One day after rewatering drought-stressed plants, I5A photosynthesis had recovered 75% versus 57% recovery with cultivar SR1 plants”
Claim · model-written summary
After 2.5 weeks of 250 mM NaCl in hydroponic solution, I5A fixed more CO2 than SR1 (4.9 versus 2.5 umol CO2 m-2 s-1).
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Material
- I5Ae3
- Treatment
- 250 mM NaCle3
- Outcome
- photosynthetic CO2 fixatione4
- Direction
- higherinferrede3
- Value
- 4.9 [plus or minus] 1.4 [mu]mol CO2 m-2 s-1e3
- Comparator
- SR1e3
- Setting
- hydroponic solutione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e3“After 2.5 weeks of 250 mM NaCl in hydroponic solution, I5A fixed 4.9 [plus or minus] 1.4 [mu]mol CO2 m-2 s-1, whereas SR1 fixed 2.5 [plus or minus] 0.6 [mu]mol CO2 m-2 s-1”
e4“In I5A, photosynthetic CO2 fixation was inhibited less during salt stress and drought”
Claim · model-written summary
Preconditioning of I5A in 50 mM NaCl increased D-ononitol amounts.
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Material
- I5Ae5
- Treatment
- Preconditioning of I5A plants in 50 mM NaCle5
- Outcome
- D-ononitol amountse5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e5“Preconditioning of I5A plants in 50 mM NaCl increased D-ononitol amounts and resulted in increased protection when the plants were stressed subsequently with 150 mM NaCl”
Claim · model-written summary
Preconditioning of I5A in 50 mM NaCl increased protection when plants were later stressed with 150 mM NaCl.
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Material
- I5Ae5
- Treatment
- 150 mM NaCle5
- Outcome
- protectione5
- Direction
- increasede5
- Qualifier
- Preconditioning of I5A plants in 50 mM NaCle5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e5“Preconditioning of I5A plants in 50 mM NaCl increased D-ononitol amounts and resulted in increased protection when the plants were stressed subsequently with 150 mM NaCl”
Claim · model-written summary
Pro, Suc, Fru and Glc showed substantial diurnal fluctuations in amounts in tobacco.
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Outcome
- substantial diurnal fluctuations in amountse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e6“Pro, Suc, Fru, and Glc showed substantial diurnal fluctuations in amounts, but D-ononitol did not”
Claim · model-written summary
D-ononitol amounts did not show diurnal fluctuations in tobacco.
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Compound
- D-ononitole6
- Outcome
- diurnal fluctuationse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e6“Pro, Suc, Fru, and Glc showed substantial diurnal fluctuations in amounts, but D-ononitol did not”
Claim · model-written summary
Stress-inducible solute accumulation appears to give better protection under drought and salt stress than constitutive osmotic adjustment.
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Treatment
- Plant transformation resulting in stress-inducible, stable solute accumulatione7
- Outcome
- protectione7
- Direction
- bettere7
- Qualifier
- under drought and salt-stress conditionse7
- Comparator
- osmotic adjustment by metabolites that are constitutively presente7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1”
e7“Plant transformation resulting in stress-inducible, stable solute accumulation appears to provide better protection under drought and salt-stress conditions than strategies using osmotic adjustment by metabolites that are constitutively present”
Claim · model-written summary
myo-Inositol, the substrate for IMT1, increases in tobacco under stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“myo-Inositol, the substrate for IMT1, increases in tobacco under stress”
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