Abstract
The introduction of new varieties and production system of anthuriums has faced some problems due to the lowmultiplication rates in conventional vegetative and genotypic alteration problem in reproductive propagation. Sincethen, in vitro propagation technique became important to be investigated. The research was carried out fromSeptember 2006 to August 2007 at the Indonesian Ornamental Crops Research Institute. A complete factorialexperiment was designed to accomplish two chronological in vitro activities. The first step dealt with differentincubation sites, i.e. dark and light conditions for callus induction of three potted anthurium accessions, namelyclone no. SM. 001, cv. Alphine and cv. Bonito. The best callus obtained from the incubation treatment were then,transferred into defined media with different formulations to get free-callus plantlets in the second steps. Theresults showed that more progressive callus development from spathe explant was detected on the media ofmodified Nitsch and Nitsch + 1 mg/l 2,D + 1 mg/l Kin + 1 mg/l BA stored at dark after 60 days incubation. After 45days transferring the callus in the same media on light conditions, torpedo-shaped callus was detected. Completeplanlets were obtained after 75 days reculturing the buds in Nitsch and Nitsch medium containing 2,4 D withvarious BA concentrations. Clone SM. 001 and cv. Bonito showed higher number of visible shoot and rootdevelopments compared to cv. Alphine. In general, better shoot and root developments and higher planlets survivedafter acclimatization were observed on the BA concentrations of 2 and 3 mg/l.
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Claims 7
Claim · model-written summary
Modified Nitsch and Nitsch medium with growth regulators in darkness yielded more progressive spathe callus after 60 days.
- Treatment
- modified Nitsch and Nitsch + 1 mg/l 2,D + 1 mg/l Kin + 1 mg/l BA stored at darke0
- Outcome
- callus development from spathe explante0
- Direction
- more progressivee0
- Value
- 60 dayse0
AnthuriumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“more progressive callus development from spathe explant was detected on the media ofmodified Nitsch and Nitsch + 1 mg/l 2,D + 1 mg/l Kin + 1 mg/l BA stored at dark after 60 days incubation”
t0“Anthurium”
Claim · model-written summary
Torpedo-shaped callus appeared after 45 days in light conditions.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 45days transferring the callus in the same media on light conditions, torpedo-shaped callus was detected”
t0“Anthurium”
Claim · model-written summary
Complete plantlets formed after 75 days of bud reculture in Nitsch and Nitsch medium with 2,4 D and BA.
- Treatment
- Nitsch and Nitsch medium containing 2,4 D withvarious BA concentrationse0
- Outcome
- Completeplanletse0
- Value
- 75 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Completeplanlets were obtained after 75 days reculturing the buds in Nitsch and Nitsch medium containing 2,4 D withvarious BA concentrations”
t0“Anthurium”
Claim · model-written summary
Clone SM. 001 developed more visible shoots and roots than cv. Alphine.
- Material
- Clone SM. 001e0
- Outcome
- visible shoot and rootdevelopmentse0
- Direction
- highere0
- Comparator
- cv. Alphinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clone SM. 001 and cv. Bonito showed higher number of visible shoot and rootdevelopments compared to cv. Alphine”
t0“Anthurium”
Claim · model-written summary
Cv. Bonito developed more visible shoots and roots than cv. Alphine.
- Material
- cv. Bonitoe0
- Outcome
- visible shoot and rootdevelopmentse0
- Direction
- highere0
- Comparator
- cv. Alphinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clone SM. 001 and cv. Bonito showed higher number of visible shoot and rootdevelopments compared to cv. Alphine”
t0“Anthurium”
Claim · model-written summary
BA at 2 mg/l produced better shoots and roots and greater plantlet survival after acclimatization.
- Treatment
- BA concentrations of 2e0
- Outcome
- shoot and root developments and higher planlets survivedafter acclimatizatione0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“better shoot and root developments and higher planlets survivedafter acclimatization were observed on the BA concentrations of 2 and 3 mg/l”
t0“Anthurium”
Claim · model-written summary
BA at 3 mg/l produced better shoots and roots and greater plantlet survival after acclimatization.
- Treatment
- 3 mg/le0
- Outcome
- shoot and root developments and higher planlets survivedafter acclimatizatione0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“better shoot and root developments and higher planlets survivedafter acclimatization were observed on the BA concentrations of 2 and 3 mg/l”
t0“Anthurium”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.