Abstract
In vitro plantlet regeneration in a number of Anthurium (Anthurium andreanum) varieties was achieved from callus cultures derived from young, tender leaf explants on Nitsch’s (1969) medium. Apart from the time period for callus induction, no differences in terms of regeneration were noted amongst the three varieties tested. Callus induction was found to be more rapid and prolific when Nitsch medium containing BA (1 mg L) and 2,4-D (0.1 mg L) with a reduced concentration of ammonium nitrate (200 mg L) was used. Shoot formation occurred when BA concentration was reduced to 0.5 mg L and the ammonium nitrate level increased to 720 mg L. Regenerated shoots rooted readily on Nitsch medium containing IBA (1.0 mg L). Rooting was improved significantly by the addition of activated charcoal (0.04%) to the medium. When explants (leaves, seeds, in vitro plantlets) were irradiated, best response was observed with the 5 Grays (Gy) treatment in terms of callus formation and regeneration while the 15 Gy dose was lethal to the Anthurium tissues. The phenotypic results indicated a boosting effect of the 5 Gy dose on the leaf tissues. The variability in the responses observed seemed to indicate some mutation, both positive and negative, at the cellular level of the tissues. However, no difference in RAPD profiles were noted between the DNA fingerprints of the mother plant and that of the irradiated tissues using a limited number of primers.
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Claims 10
Claim · model-written summary
Three varieties did not differ in regeneration.
AnthuriumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e0“no differences in terms of regeneration were noted amongst the three varieties tested.”
Claim · model-written summary
The specified Nitsch medium accelerated and increased callus induction.
- Treatment
- Nitsch medium containing BA (1 mg L) and 2,4-D (0.1 mg L) with a reduced concentration of ammonium nitrate (200 mg L)e1
- Outcome
- Callus inductione1
- Direction
- more rapid and prolifice1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e1“Callus induction was found to be more rapid and prolific when Nitsch medium containing BA (1 mg L) and 2,4-D (0.1 mg L) with a reduced concentration of ammonium nitrate (200 mg L) was used.”
Claim · model-written summary
Reducing BA and increasing ammonium nitrate produced shoots.
- Treatment
- BA concentration was reduced to 0.5 mg L and the ammonium nitrate level increased to 720 mg Le2
- Outcome
- Shoot formatione2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e2“Shoot formation occurred when BA concentration was reduced to 0.5 mg L and the ammonium nitrate level increased to 720 mg L.”
Claim · model-written summary
Regenerated shoots rooted readily with IBA medium.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e3“Regenerated shoots rooted readily on Nitsch medium containing IBA (1.0 mg L). Rooting was improved significantly by the addition of activated charcoal (0.04%) to the medium.”
Claim · model-written summary
Activated charcoal improved rooting significantly.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e3“Regenerated shoots rooted readily on Nitsch medium containing IBA (1.0 mg L). Rooting was improved significantly by the addition of activated charcoal (0.04%) to the medium.”
Claim · model-written summary
5 Gy gave the best callus formation and regeneration response.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e4“best response was observed with the 5 Grays (Gy) treatment in terms of callus formation and regeneration while the 15 Gy dose was lethal to the Anthurium tissues.”
Claim · model-written summary
15 Gy was lethal to Anthurium tissues.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“best response was observed with the 5 Grays (Gy) treatment in terms of callus formation and regeneration while the 15 Gy dose was lethal to the Anthurium tissues.”
Claim · model-written summary
The 5 Gy dose boosted leaf tissue phenotype.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e5“The phenotypic results indicated a boosting effect of the 5 Gy dose on the leaf tissues.”
Claim · model-written summary
Variable responses suggested positive and negative cellular mutations.
- Outcome
- some mutation, both positive and negative, at the cellular level of the tissuese6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e6“The variability in the responses observed seemed to indicate some mutation, both positive and negative, at the cellular level of the tissues. However, no difference in RAPD profiles were noted between the DNA fingerprints of the mother plant and that of the irradiated tissues using a limited number of primers.”
Claim · model-written summary
RAPD profiles did not differ between mother plant and irradiated tissues with limited primers.
- Treatment
- irradiated tissuese6
- Outcome
- RAPD profilese6
- Direction
- no differencee6
- Qualifier
- using a limited number of primerse6
- Comparator
- mother plante6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ANTHURIUM”
e6“The variability in the responses observed seemed to indicate some mutation, both positive and negative, at the cellular level of the tissues. However, no difference in RAPD profiles were noted between the DNA fingerprints of the mother plant and that of the irradiated tissues using a limited number of primers.”
The same questions in other papers
charcoal · growth · AnthuriumResults agree
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- positive
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2,4-D · growth · AnthuriumResults agree
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- positive
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N-benzyladenine · growth · AnthuriumResults agree
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- positive
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See all 6 papers →in vitro · growth · AnthuriumPositive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.