Abstract
Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar) was employed to evaluate the importance of glutathione in hydrogen peroxide (H2O2)-induced chilling tolerance. Seeds germinated at 25°C for 7d were subjected to different periods of chilling treatment, prior to analysis of the glutathione contents of their leaves. In a comparison of acclimation temperatures from 2-12°C, it was found that an 8°C acclimation for 36 h induced a 5.7-fold increase, the highest glutathione level among the temperatures tested. Seedlings acclimated at 8°C for 36 h showed 97% survival after a 36-h, 4°C chilling stress, compared with 33% survival of non-acclimated plants. Pretreatment with 200 mM H2O2 for 12 h before a 36-h, 4°C chilling treatment increased glutathione levels by 30% and reduced electrolyte leakage to 43%, relative to the untreated control. Treated seedlings also showed a survival rate of 71% after the same chilling treatment. Application of 1 mM buthionine sulfoximine, a specific inhibitor of glutathione synthesis, reversed the protection against chilling stress provided to seedlings either by acclimation at 8°C for 36 h or H2O2 pretreatment. The role of glutathione in chilling acclimation or H2O2-pretreatment-induced chilling tolerance is thus confirmed.
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Claims 7
Claim · model-written summary
An 8°C acclimation for 36 h raised leaf glutathione 5.7-fold, the highest level among the acclimation temperatures tested (2-12°C).
- Taxon
- Mung bean
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Vigna radiatae0 - Material
- cv. TN5e0
- Organ / stage
- leavese2
- Treatment
- 8°C acclimation for 36 he1
- Outcome
- glutathione levele1
- Direction
- increasee1
- Value
- 5.7-folde1
- Comparator
- acclimation temperatures from 2-12°Ce1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar)”
e1“In a comparison of acclimation temperatures from 2-12°C, it was found that an 8°C acclimation for 36 h induced a 5.7-fold increase, the highest glutathione level among the temperatures tested”
e2“prior to analysis of the glutathione contents of their leaves”
Claim · model-written summary
Acclimation at 8°C for 36 h raised survival after a 36-h 4°C chilling stress to 97%, against 33% in non-acclimated seedlings.
- Taxon
- Mung beanVigna radiatae0
- Treatment
- acclimated at 8°C for 36 he3
- Outcome
- survival after a 36-h, 4°C chilling stresse3
- Direction
- higherinferrede3
- Value
- 97%e3
- Qualifier
- 33% survival of non-acclimated plantse3
- Comparator
- non-acclimated plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar)”
e3“Seedlings acclimated at 8°C for 36 h showed 97% survival after a 36-h, 4°C chilling stress, compared with 33% survival of non-acclimated plants”
Claim · model-written summary
H2O2 pretreatment (200 mM, 12 h) raised leaf glutathione by 30% relative to the untreated control.
- Taxon
- Mung beanVigna radiatae0
- Treatment
- Pretreatment with 200 mM H2O2 for 12 he4
- Outcome
- glutathione levelse4
- Direction
- increasede4
- Value
- 30%e4
- Comparator
- untreated controle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar)”
e4“Pretreatment with 200 mM H2O2 for 12 h before a 36-h, 4°C chilling treatment increased glutathione levels by 30% and reduced electrolyte leakage to 43%, relative to the untreated control”
Claim · model-written summary
H2O2 pretreatment (200 mM, 12 h) reduced electrolyte leakage to 43% relative to the untreated control under chilling.
- Taxon
- Mung beanVigna radiatae0
- Treatment
- Pretreatment with 200 mM H2O2 for 12 he4
- Outcome
- electrolyte leakagee4
- Direction
- reducede4
- Value
- 43%e4
- Comparator
- untreated controle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar)”
e4“Pretreatment with 200 mM H2O2 for 12 h before a 36-h, 4°C chilling treatment increased glutathione levels by 30% and reduced electrolyte leakage to 43%, relative to the untreated control”
Claim · model-written summary
H2O2-pretreated seedlings showed 71% survival after the same chilling treatment; the comparator is not stated.
- Taxon
- Mung beanVigna radiatae0
- Treatment
- Pretreatment with 200 mM H2O2 for 12 he4
- Outcome
- survival ratee5
- Value
- 71%e5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar)”
e4“Pretreatment with 200 mM H2O2 for 12 h before a 36-h, 4°C chilling treatment increased glutathione levels by 30% and reduced electrolyte leakage to 43%, relative to the untreated control”
e5“Treated seedlings also showed a survival rate of 71% after the same chilling treatment”
Claim · model-written summary
Application of 1 mM buthionine sulfoximine, an inhibitor of glutathione synthesis, reversed the chilling protection provided by acclimation or H2O2 pretreatment.
- Taxon
- Mung beanVigna radiatae0
- Treatment
- Application of 1 mM buthionine sulfoximinee6
- Compound
- glutathionee6
- Outcome
- protection against chilling stresse6
- Direction
- reversede6
- Comparator
- seedlings either by acclimation at 8°C for 36 h or H2O2 pretreatmente6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar)”
e6“Application of 1 mM buthionine sulfoximine, a specific inhibitor of glutathione synthesis, reversed the protection against chilling stress provided to seedlings either by acclimation at 8°C for 36 h or H2O2 pretreatment”
Claim · model-written summary
Glutathione is asserted to underlie chilling acclimation and H2O2-induced chilling tolerance.
- Taxon
- Mung beanVigna radiatae0
- Compound
- glutathionee7
- Outcome
- chilling tolerancee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar)”
e7“The role of glutathione in chilling acclimation or H2O2-pretreatment-induced chilling tolerance is thus confirmed”
The same questions in other papers
hydrogen peroxide · molecular: metabolites · Vigna radiata (mung bean)Positive and negative results
1 reporting no difference from an alternative
- no effect
hydrogen peroxide · survival · Vigna radiata (mung bean)Results agree
1 paper in the same direction as this paper
- positive
hydrogen peroxide · physiology: oxidative · Vigna radiata (mung bean)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.