Abstract
Osmopriming is a pre-sowing treatment that enhances germination performance and stress tolerance of germinating seeds. Brassica napus seeds showed osmopriming-improved germination and seedling growth under salinity stress. To understand the molecular and biochemical mechanisms of osmopriming-induced salinity tolerance, the accumulation of proline, gene expression and activity of enzymes involved in proline metabolism and the level of endogenous hydrogen peroxide were investigated in rape seeds during osmopriming and post-priming germination under control (H2O) and stress conditions (100 mM NaCl). The relationship between gene expression and enzymatic activity of pyrroline-5-carboxylate synthetase (P5CS), ornithine-δ-aminotransferase (OAT) and proline dehydrogenase (PDH) was determined. The improved germination performance of osmoprimed seeds was accompanied by a significant increase in proline content. The accumulation of proline during priming and post-priming germination was associated with strong up-regulation of the P5CSA gene, down-regulation of the PDH gene and accumulation of hydrogen peroxide. The up-regulated transcript level of P5CSA was consistent with the increase in P5CS activity. This study shows, for the first time, the role of priming-induced modulation of activities of particular genes and enzymes of proline turnover, and its relationship with higher content of hydrogen peroxide, in improving seed germination under salinity stress. Following initial stress-exposure, the primed seeds acquired stronger salinity stress tolerance during post-priming germination, a feature likely linked to a 'priming memory'.
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Claims 8
Claim · model-written summary
Osmopriming improved Brassica germination and seedling growth under salinity stress.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Treatment
- osmopriminge0
- Outcome
- germination and seedling growthe0
- Direction
- improvede0
- Qualifier
- under salinity stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Brassica napus seeds showed osmopriming-improved germination and seedling growth under salinity stress.”
Claim · model-written summary
Improved germination of osmoprimed seeds accompanied a significant increase in proline.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- osmoprimede0
- Outcome
- proline contente0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The improved germination performance of osmoprimed seeds was accompanied by a significant increase in proline content.”
t0“Brassica napus”
Claim · model-written summary
Proline accumulation during priming and germination was associated with P5CSA up-regulation.
- Taxon
- Brassica napusBrassica napust0
- Gene
- P5CSAe0
- Outcome
- P5CSA genee0
- Direction
- up-regulatione0
- Qualifier
- accumulation of prolinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of proline during priming and post-priming germination was associated with strong up-regulation of the P5CSA gene, down-regulation of the PDH gene and accumulation of hydrogen peroxide.”
t0“Brassica napus”
Claim · model-written summary
Proline accumulation during priming and germination was associated with PDH down-regulation.
- Taxon
- Brassica napusBrassica napust0
- Gene
- PDHe0
- Outcome
- PDH genee0
- Direction
- down-regulatione0
- Qualifier
- accumulation of prolinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of proline during priming and post-priming germination was associated with strong up-regulation of the P5CSA gene, down-regulation of the PDH gene and accumulation of hydrogen peroxide.”
t0“Brassica napus”
Claim · model-written summary
Proline accumulation during priming and germination was associated with hydrogen peroxide accumulation.
- Taxon
- Brassica napusBrassica napust0
- Compound
- hydrogen peroxidee0
- Outcome
- accumulation of prolinee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of proline during priming and post-priming germination was associated with strong up-regulation of the P5CSA gene, down-regulation of the PDH gene and accumulation of hydrogen peroxide.”
t0“Brassica napus”
Claim · model-written summary
P5CSA transcript up-regulation coincided with increased P5CS activity.
- Taxon
- Brassica napusBrassica napust0
- Gene
- P5CSAe0
- Outcome
- P5CS activitye0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The up-regulated transcript level of P5CSA was consistent with the increase in P5CS activity.”
t0“Brassica napus”
Claim · model-written summary
After initial stress exposure, primed seeds acquired stronger salinity tolerance during germination.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- primed seedse0
- Outcome
- salinity stress tolerancee0
- Direction
- strongere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Following initial stress-exposure, the primed seeds acquired stronger salinity stress tolerance during post-priming germination”
t0“Brassica napus”
Claim · model-written summary
The authors suggest that priming memory may underlie acquired salinity tolerance.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- priming memorye0
- Qualifier
- likely linked to a 'priming memory'e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a feature likely linked to a 'priming memory'”
t0“Brassica napus”
The same questions in other papers
seed treatment · growth · Brassica napus (oilseed rape)Results differ
63 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
66 papers more in this group
See all 70 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.