Abstract
An endogenous inhibitor of the in vitro binding of the phytotropin N-1-naphthylphthalamic acid to microsomal membranes was detected in extracts prepared from etiolated pea (Pisum sativum L.) epicotyls. Following extensive purification, the inhibitor was identified as linoleic acid. Authentic linoleic acid inhibited N-1-naphthylphthalamic acid binding noncompetitively in a dose-dependent manner, exhibiting a 50% inhibitory concentration of approximately 24 ([mu]M. Using a variety of fatty acids and their derivatives, this inhibition was found to exhibit strict structural requirements, with both linoleic and linolenic acids being the most inhibitory. A variety of membrane-solubilizing detergents elicited no such inhibitory activity when tested at equivalent concentrations. The possible physiological significance of this interaction is discussed and it is proposed that linoleic acid serves as an intracellular modulator of phytotropin binding and therefore polar auxin transport.
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Claims 5
Claim · model-written summary
An endogenous binding inhibitor was detected in pea epicotyl extracts.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- epicotylse0
- Outcome
- endogenous inhibitore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An endogenous inhibitor of the in vitro binding of the phytotropin N-1-naphthylphthalamic acid to microsomal membranes was detected in extracts prepared from etiolated pea (Pisum sativum L.) epicotyls”
Claim · model-written summary
The inhibitor was identified as linoleic acid.
- Outcome
- linoleic acide0
Not reportedTaxon
Verbatim
e0“the inhibitor was identified as linoleic acid”
Claim · model-written summary
Linoleic acid inhibited binding noncompetitively with a 50% inhibitory concentration of approximately 24 ([mu]M.
- Treatment
- linoleic acide0
- Outcome
- N-1-naphthylphthalamic acid bindinge0
- Direction
- inhibitede0
- Value
- approximately 24 ([mu]Me0
Not reportedTaxonComparator
Verbatim
e0“Authentic linoleic acid inhibited N-1-naphthylphthalamic acid binding noncompetitively in a dose-dependent manner, exhibiting a 50% inhibitory concentration of approximately 24 ([mu]M”
Claim · model-written summary
Inhibition had strict structural requirements, with linoleic and linolenic acids most inhibitory.
Not reportedTaxon
Verbatim
e0“this inhibition was found to exhibit strict structural requirements, with both linoleic and linolenic acids being the most inhibitory”
Claim · model-written summary
Detergents showed no inhibitory activity at equivalent concentrations.
Not reportedTaxonComparator
Verbatim
e0“A variety of membrane-solubilizing detergents elicited no such inhibitory activity when tested at equivalent concentrations”