Abstract
Differential display 방법으로 NaCl에 의하여 발현이 증가되는 cDNA들을 분리하였으며 그중 하나가 식물유래의 outer envelope membrane protein과 높은 유사성을 보였으므로 이를 ShOEP로 명명하였다. ShOEP는 1293 bp 길이에 359개의 아미노산으로 구성된 open reading frame을 포함하고 있으며, 이로부터 추정되는 분자량은 8.9 kDa이었다. ShOEP 단백질은 애기장대의 OEP와는 40.6%, 시금치와는 38%의 유사성을 나타내었다. Northern 분석결과, ShOEP 유전자는 NaCl의 농도가 증가함에 따라 발현량이 급격히 증가하는 것으로 나타났다. 염생식물인 퉁퉁마디의 OEP는 PEG에 의하여 발현이 증가하는 반면 비염생식물인 애기장대의 OEP는 큰 차이를 보이지 않았다. 효모 complementation 실험결과 ShOEP는 NaCl에 특이적이었으며 식물의 염분스트레스 내성 기작에 직접적으로 관여하고 있음을 알 수 있었다. Complementary DNA encoding chloroplast outer envelope membrane protein (OEP) from the halophyte Salicornia herbacea has been cloned and sequenced. The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa. The expression level of ShOEP increased by salt, drought and ABA treatments. ShOEP expression was largely induced in roots and shoots by high salts. The biological function of ShOEP was examined by yeast complementation. ShOEP can suppress Na$^{+}$ sensitivity of yeast mutant (cnb$\Delta$) in the presence of salt. These results suggest that ShOEP is a salt inducible gene and may have functions in the regulation of plant salt stress.ant salt stress.
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Claims 9
Claim · model-written summary
ShOEP cDNA was cloned and sequenced from Salicornia herbacea.
- Taxon
- Salicornia herbacea
_01.jpg?width=60)
Salicornia europaeae0 - Gene
- OEPe0
- Outcome
- cloned and sequencede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_01.jpg?width=500)
Verbatim
e0“Complementary DNA encoding chloroplast outer envelope membrane protein (OEP) from the halophyte Salicornia herbacea has been cloned and sequenced.”
Claim · model-written summary
The full-length ShOEP cDNA was 596 bp.
- Taxon
- Salicornia herbaceaSalicornia europaeat0
- Outcome
- full length cDNAe0
- Value
- 596 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salicornia herbacea”
e0“The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa.”
Claim · model-written summary
ShOEP encoded 91 amino acid residues.
- Taxon
- Salicornia herbaceaSalicornia europaeat0
- Outcome
- polypeptidee0
- Value
- 91 amino acid residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salicornia herbacea”
e0“The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa.”
Claim · model-written summary
The predicted polypeptide molecular mass was 8.9 kDa.
- Taxon
- Salicornia herbaceaSalicornia europaeat0
- Outcome
- molecular masse0
- Value
- 8.9 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salicornia herbacea”
e0“molecular mass of 8.9 kDa”
Claim · model-written summary
ShOEP expression increased with salt treatment.
- Taxon
- Salicornia herbaceaSalicornia europaeat0
- Treatment
- salte0
- Gene
- ShOEPe0
- Outcome
- expression level of ShOEPe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salicornia herbacea”
e0“The expression level of ShOEP increased by salt, drought and ABA treatments.”
Claim · model-written summary
ShOEP expression increased with drought treatment.
- Taxon
- Salicornia herbaceaSalicornia europaeat0
- Treatment
- droughte0
- Gene
- ShOEPe0
- Outcome
- expression level of ShOEPe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salicornia herbacea”
e0“The expression level of ShOEP increased by salt, drought and ABA treatments.”
Claim · model-written summary
ShOEP expression increased with ABA treatment.
- Taxon
- Salicornia herbaceaSalicornia europaeat0
- Treatment
- ABAe0
- Gene
- ShOEPe0
- Outcome
- expression level of ShOEPe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salicornia herbacea”
e0“The expression level of ShOEP increased by salt, drought and ABA treatments.”
Claim · model-written summary
High salts largely induced ShOEP expression in roots and shoots.
- Taxon
- Salicornia herbaceaSalicornia europaeat0
- Organ / stage
- roots and shootse0
- Treatment
- high saltse0
- Gene
- ShOEPe0
- Outcome
- ShOEP expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salicornia herbacea”
e0“ShOEP expression was largely induced in roots and shoots by high salts.”
Claim · model-written summary
ShOEP suppressed yeast mutant salt sensitivity in complementation.
- Taxon
- Salicornia herbaceaSalicornia europaeat0
- Treatment
- salte0
- Gene
- ShOEPe0
- Outcome
- Na$^{+}$ sensitivity of yeast mutant (cnb$\Delta$)e0
- Direction
- suppresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salicornia herbacea”
e0“ShOEP can suppress Na$^{+}$ sensitivity of yeast mutant (cnb$\Delta$) in the presence of salt.”
The same questions in other papers
salinity · molecular: expression · Salicornia europaea (common glasswort)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.