Abstract
The micropropagation of grapevine cvs Vugava and Plavac mali throught nodal segment method was studied. For culture establishment green, one-node cutings from the vineyard plants were used. Murashinge and Skoog (MS) medium supplemented with 4.4 microM was the most useful to induce bud growth in buth cultivars. After two to four weeks of culture, axillary shoots were excised from nodal segments and transferred to the sama basal medium with the addition of 11.4 microM IAA for for further four weeks. This method was sutable for the culture establishment of both cultivars. Growing shoots were excised to obtain new one-node sterile minicuttings that were again cultured. The average number of nodes per shoot through seven successive subcultures was 4.4. Nodal segment method was suitable for the micropropagation of Vugava cultivar. 6-benzyladenine was more efficient in axillary bud induction in comparison with kinetin. In Vugava cultures optimum shoot and root growth was obtained on MS medium supplemented with 11.4 microM IBA ; the growth of axillary buds was faster in younger nodes of the donor plant. Nodal segment method has a potential value in commercial clonal propagation of Vugava cultivar. In the culture of Plavac mali, shoot growth was inconsistent and in vitro propagation was strongly limited.
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Claims 10
Claim · model-written summary
MS medium supplemented with 4.4 microM was most useful for bud growth in both cultivars.
- Material
- Vugava and Plavac malie1
- Treatment
- Murashinge and Skoog (MS) medium supplemented with 4.4 microMe0
- Outcome
- bud growthe0
- Direction
- most usefule0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Murashinge and Skoog (MS) medium supplemented with 4.4 microM was the most useful to induce bud growth in buth cultivars.”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
The nodal segment protocol was suitable for establishing cultures of both cultivars.
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This method was sutable for the culture establishment of both cultivars.”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
The mean number of nodes per shoot across seven subcultures was 4.4.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average number of nodes per shoot through seven successive subcultures was 4.4.”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
The nodal segment method was suitable for Vugava micropropagation.
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nodal segment method was suitable for the micropropagation of Vugava cultivar.”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
6-benzyladenine induced axillary buds more efficiently than kinetin.
- Treatment
- 6-benzyladeninee0
- Outcome
- axillary bud inductione0
- Direction
- more efficiente0
- Comparator
- kinetine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6-benzyladenine was more efficient in axillary bud induction in comparison with kinetin.”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
MS medium with 11.4 microM IBA gave optimum shoot and root growth in Vugava.
- Material
- Vugavae0
- Treatment
- MS medium supplemented with 11.4 microM IBAe0
- Outcome
- shoot and root growthe0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Vugava cultures optimum shoot and root growth was obtained on MS medium supplemented with 11.4 microM IBA”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
Axillary buds grew faster in younger donor plant nodes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the growth of axillary buds was faster in younger nodes of the donor plant”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
The nodal segment method was considered promising for commercial clonal propagation of Vugava.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nodal segment method has a potential value in commercial clonal propagation of Vugava cultivar.”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
Plavac mali shoot growth was inconsistent.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the culture of Plavac mali, shoot growth was inconsistent”
e1“grapevine cvs Vugava and Plavac mali”
Claim · model-written summary
Plavac mali in vitro propagation was strongly limited.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in vitro propagation was strongly limited.”
e1“grapevine cvs Vugava and Plavac mali”
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See all 54 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.