Paper · 2015 · AFRICAN JOURNAL OF BIOTECHNOLOGY

In vitro regeneration and induction of multiple shooting in Cicer arietinum L. using cotyledonary nodal explants

Prema Sunil Sruthi; Philip Robinson J; S KarthickBalan S; et al.

7
claims
13
verbatim quotes
11
citations
2
research areas
✓
Open access · cc-by

Abstract

Chickpea, a temperate crop, is the world’s third most important pulse crop and India produces 75% of world’s supply. The two most common types of Chickpea are the white-seeded "Kabuli" and the "Desi "variety. In vitro regeneration was achieved using cotyledonary nodal explants using desi variety seeds of cultivar K850 of Cicer arietinum. The multiple shoot induction was observed with various concentrations of auxins and cytokinins. The maximum proliferation was found at 0.5 mg/L 6-benzylaminopurine (BAP). The effectiveness was observed at various combinations. Maximum efficiency of multiple shooting was obtained at 0.5 mg/L BAP and 0.05 mg/L 2,4-dichlorophenoxy acetic acid (2,4D). For root induction, well developed shoots were transferred into rooting medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA); maximum response was found at concentrations 0.10 and 0.50 mg/L, respectively. Successful rooting was recorded on media supplemented with 0.1 mg/L IBA. The well rooted plants were transferred into the soil. Key words: Explants, multiple shooting, phytohormones and acclimatization.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

K850 chickpea was regenerated in vitro from cotyledonary nodal explants.

Taxon
Cicer arietinumCicer arietinume0
Material
K850e0
Organ / stage
cotyledonary nodal explantse0
Outcome
In vitro regeneratione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In vitro regeneration was achieved using cotyledonary nodal explants using desi variety seeds of cultivar K850 of Cicer arietinum.”

✓ located verbatim in the source · abstract, characters 209–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Multiple shoot induction occurred with various auxin and cytokinin concentrations.

Taxon
Cicer arietinum L.Cicer arietinumt0
Treatment
various concentrations of auxins and cytokininse0
Outcome
multiple shoot inductione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The multiple shoot induction was observed with various concentrations of auxins and cytokinins.”

✓ located verbatim in the source · abstract, characters 340–435 · show in text ↑
t0

“Cicer arietinum L.”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Maximum proliferation occurred at 0.5 mg/L BAP.

Taxon
Cicer arietinum L.Cicer arietinumt0
Treatment
0.5 mg/L 6-benzylaminopurine (BAP)e0
Outcome
proliferatione0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The maximum proliferation was found at 0.5 mg/L 6-benzylaminopurine (BAP).”

✓ located verbatim in the source · abstract, characters 436–510 · show in text ↑
t0

“Cicer arietinum L.”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Maximum multiple shooting efficiency occurred with 0.5 mg/L BAP plus 0.05 mg/L 2,4D.

Taxon
Cicer arietinum L.Cicer arietinumt0
Treatment
0.5 mg/L BAP and 0.05 mg/L 2,4-dichlorophenoxy acetic acid (2,4D)e0
Outcome
efficiency of multiple shootinge0
Direction
Maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Maximum efficiency of multiple shooting was obtained at 0.5 mg/L BAP and 0.05 mg/L 2,4-dichlorophenoxy acetic acid (2,4D).”

✓ located verbatim in the source · abstract, characters 567–689 · show in text ↑
t0

“Cicer arietinum L.”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Maximum root induction response to IBA occurred at 0.10 mg/L.

Taxon
Cicer arietinum L.Cicer arietinumt0
Treatment
indole-3-butyric acid (IBA)e0
Outcome
root inductione0
Direction
maximume0
Value
0.10e0
Qualifier
mg/Le0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“For root induction, well developed shoots were transferred into rooting medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA); maximum response was found at concentrations 0.10 and 0.50 mg/L, respectively.”

✓ located verbatim in the source · abstract, characters 690–925 · show in text ↑
t0

“Cicer arietinum L.”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Maximum root induction response to IAA occurred at 0.50 mg/L.

Taxon
Cicer arietinum L.Cicer arietinumt0
Treatment
indole-3-acetic acid (IAA)e0
Outcome
root inductione0
Direction
maximume0
Value
0.50 mg/Le0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“For root induction, well developed shoots were transferred into rooting medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA); maximum response was found at concentrations 0.10 and 0.50 mg/L, respectively.”

✓ located verbatim in the source · abstract, characters 690–925 · show in text ↑
t0

“Cicer arietinum L.”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Successful rooting was recorded with 0.1 mg/L IBA.

Taxon
Cicer arietinum L.Cicer arietinumt0
Treatment
0.1 mg/L IBAe0
Outcome
Successful rootinge0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Successful rooting was recorded on media supplemented with 0.1 mg/L IBA.”

✓ located verbatim in the source · abstract, characters 926–998 · show in text ↑
t0

“Cicer arietinum L.”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑

indole-3-butyric acid · growth · Cicer arietinum (chickpea)Results agree

12 papers: 12 positive, 0 negative or no effect

11 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

class · growth · Cicer arietinum (chickpea)Positive and negative results

15 papers: 14 positive, 1 negative or no effect

13 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. positive

11 papers more in this group

See all 15 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.