Abstract
Studies were carried out for rapid micropropagation of three elite sugarcane varieties i.e., HSF-240, CP-77-400 and CPF-237. The explants were surface sterilized with 50% clorox for 30 minutes. The cultures were initiated by inoculating them on MS (Murashige & Skoog, 1962) medium containing 1.0 mg/l Kin along with 0.1 mg/l GA3. Multiplication of the cultures was obtained by using BAP and Kin in various combinations and concentrations in MS medium. The optimum multiplication for variety HSF-240 was obtained at 1.5 mg/l BAP, 0.5 mg/l Kin with 16.5 cm shoot length, 11 number of tillers and 32 number of leaves per plant. Similarly optimum multiplication for variety CP-77-400 was obtained at 1.0 mg/l BAP and 0.5 mg/l Kin, with a maximum of 8.5 cm shoot length, 7 number of tillers and 24 number of leaves. Best multiplication rate for variety CPF-237 was observed at 1.0 mg/l BAP and 0.1 mg/l Kin with a maximum of 12 cm shoot length, 6 number of tillers and 18 leaves per plant. Rooting of the plantlets was obtained on half strength MS medium containing 6% sucrose and various concentrations of IBA.
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Claims 13
Claim · model-written summary
HSF-240 optimum multiplication occurred at 1.5 mg/l BAP and 0.5 mg/l Kin.
- Taxon
- sugarcanet0
- Material
- HSF-240e0
- Treatment
- 1.5 mg/l BAP, 0.5 mg/l Kine0
- Outcome
- multiplicatione0
- Direction
- optimume0
Not reportedComparator
Verbatim
e0“The optimum multiplication for variety HSF-240 was obtained at 1.5 mg/l BAP, 0.5 mg/l Kin with 16.5 cm shoot length, 11 number of tillers and 32 number of leaves per plant.”
t0“sugarcane”
Claim · model-written summary
HSF-240 had 16.5 cm shoot length at its optimum cytokinin treatment.
- Taxon
- sugarcanet0
- Material
- HSF-240e0
- Treatment
- 1.5 mg/l BAP, 0.5 mg/l Kine0
- Outcome
- shoot lengthe0
- Value
- 16.5 cme0
Not reportedDirection
Verbatim
e0“The optimum multiplication for variety HSF-240 was obtained at 1.5 mg/l BAP, 0.5 mg/l Kin with 16.5 cm shoot length, 11 number of tillers and 32 number of leaves per plant.”
t0“sugarcane”
Claim · model-written summary
HSF-240 had 11 tillers at its optimum treatment.
- Taxon
- sugarcanet0
- Material
- HSF-240e0
- Treatment
- 1.5 mg/l BAP, 0.5 mg/l Kine0
- Outcome
- number of tillerse0
- Value
- 11e0
Not reportedDirection
Verbatim
e0“The optimum multiplication for variety HSF-240 was obtained at 1.5 mg/l BAP, 0.5 mg/l Kin with 16.5 cm shoot length, 11 number of tillers and 32 number of leaves per plant.”
t0“sugarcane”
Claim · model-written summary
HSF-240 had 32 leaves per plant at its optimum treatment.
- Taxon
- sugarcanet0
- Material
- HSF-240e0
- Treatment
- 1.5 mg/l BAP, 0.5 mg/l Kine0
- Outcome
- number of leaves per plante0
- Value
- 32e0
Not reportedDirection
Verbatim
e0“The optimum multiplication for variety HSF-240 was obtained at 1.5 mg/l BAP, 0.5 mg/l Kin with 16.5 cm shoot length, 11 number of tillers and 32 number of leaves per plant.”
t0“sugarcane”
Claim · model-written summary
CP-77-400 optimum multiplication occurred at 1.0 mg/l BAP and 0.5 mg/l Kin.
- Taxon
- sugarcanet0
- Material
- CP-77-400e0
- Treatment
- 1.0 mg/l BAP and 0.5 mg/l Kine0
- Outcome
- multiplicatione0
- Direction
- optimume0
Not reportedComparator
Verbatim
e0“Similarly optimum multiplication for variety CP-77-400 was obtained at 1.0 mg/l BAP and 0.5 mg/l Kin, with a maximum of 8.5 cm shoot length, 7 number of tillers and 24 number of leaves.”
t0“sugarcane”
Claim · model-written summary
CP-77-400 maximum shoot length was 8.5 cm.
Verbatim
e0“Similarly optimum multiplication for variety CP-77-400 was obtained at 1.0 mg/l BAP and 0.5 mg/l Kin, with a maximum of 8.5 cm shoot length, 7 number of tillers and 24 number of leaves.”
t0“sugarcane”
Claim · model-written summary
CP-77-400 maximum tiller count was 7.
Verbatim
e0“Similarly optimum multiplication for variety CP-77-400 was obtained at 1.0 mg/l BAP and 0.5 mg/l Kin, with a maximum of 8.5 cm shoot length, 7 number of tillers and 24 number of leaves.”
t0“sugarcane”
Claim · model-written summary
CP-77-400 maximum leaf count was 24.
Verbatim
e0“Similarly optimum multiplication for variety CP-77-400 was obtained at 1.0 mg/l BAP and 0.5 mg/l Kin, with a maximum of 8.5 cm shoot length, 7 number of tillers and 24 number of leaves.”
t0“sugarcane”
Claim · model-written summary
CPF-237 best multiplication occurred at 1.0 mg/l BAP and 0.1 mg/l Kin.
- Taxon
- sugarcanet0
- Material
- CPF-237e0
- Treatment
- 1.0 mg/l BAP and 0.1 mg/l Kine0
- Outcome
- multiplication ratee0
- Direction
- Beste0
Not reportedComparator
Verbatim
e0“Best multiplication rate for variety CPF-237 was observed at 1.0 mg/l BAP and 0.1 mg/l Kin with a maximum of 12 cm shoot length, 6 number of tillers and 18 leaves per plant.”
t0“sugarcane”
Claim · model-written summary
CPF-237 maximum shoot length was 12 cm.
Verbatim
e0“Best multiplication rate for variety CPF-237 was observed at 1.0 mg/l BAP and 0.1 mg/l Kin with a maximum of 12 cm shoot length, 6 number of tillers and 18 leaves per plant.”
t0“sugarcane”
Claim · model-written summary
CPF-237 maximum tiller count was 6.
Verbatim
e0“Best multiplication rate for variety CPF-237 was observed at 1.0 mg/l BAP and 0.1 mg/l Kin with a maximum of 12 cm shoot length, 6 number of tillers and 18 leaves per plant.”
t0“sugarcane”
Claim · model-written summary
CPF-237 maximum leaf count was 18 per plant.
Verbatim
e0“Best multiplication rate for variety CPF-237 was observed at 1.0 mg/l BAP and 0.1 mg/l Kin with a maximum of 12 cm shoot length, 6 number of tillers and 18 leaves per plant.”
t0“sugarcane”
Claim · model-written summary
Plantlets rooted on half strength MS with sucrose and IBA.
- Taxon
- sugarcanet0
- Treatment
- half strength MS medium containing 6% sucrose and various concentrations of IBAe0
- Outcome
- Rooting of the plantletse0
Not reportedDirection
Verbatim
e0“Rooting of the plantlets was obtained on half strength MS medium containing 6% sucrose and various concentrations of IBA.”
t0“sugarcane”
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