Paper · 2010

MICROPROPAGATION OF SUGARCANE THROUGH LEAF SHEATH CULTURE

H. M. Tarique; Mohammad Abdul Mannan; Md. Shahidur Rashid Bhuiyan; et al.

8
claims
16
verbatim quotes
10
citations
2
research areas
—
Not open access

Abstract

Tarique, H. M., Mannan, M. A., Bhuiyan, M. S. R. and Rahaman, M. M. 2010. Micropropagation of sugarcane through leaf sheath culture. Int. J. Sustain. Crop Prod. 5(2):13-15. An experiment was conducted to develop an efficient protocol for micropropagation of sugarcane using leaf sheath culture method of three sugarcane varieties viz. Isd16, Isd36 and Isd37 in the germplasm laboratory of breeding division, Bangladesh Sugarcane Research Institute (BSRI), Ishurdi, Pabna. Leaf sheath explants were used for callus formation followed by shoot initiation. Basic medium Murashige and Skooge (MS) supplemented with different concentration of auxin (NAA and 2, 4-D) were used to observe their effects on callus formation. Best results were observed from the varieties Isd36 and Isd37 at 4.0 mg/l of 2, 4-D and the variety Isd16 at 3.0 mg/l of 2, 4-D for callus induction. Different combinations of BAP with NAA or IBA supplemented with MS medium were used for shoot initiation and multiplication. 1.0 mg/l BAP + 0.5 mg/l NAA showed the best result for induction and multiplication of shoot. MS medium supplemented with different concentrations of auxin (NAA and IBA) were used alone for in vitro root formation from proliferated shoots. NAA showed better performance than IBA for root initiation. Best result of root formation was observed on MS medium supplemented with 5.0 mg/l of NAA. The variety Isd37 produced the highest number of roots (13.47) at 5.0 mg/l of NAA. The plantlets were successfully transferred to soil with eighty to ninety percent survivability at normal temperature with 85% humidity.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Variety Isd36 gave the best callus induction at 4.0 mg/l 2,4-D.

Taxon
SUGARCANEt0
Material
Isd36e0
Treatment
4.0 mg/l of 2, 4-De0
Outcome
callus inductione0
Direction
Best resultse0

Not reportedComparator

Verbatim

e0

“Best results were observed from the varieties Isd36 and Isd37 at 4.0 mg/l of 2, 4-D and the variety Isd16 at 3.0 mg/l of 2, 4-D for callus induction”

✓ located verbatim in the source · abstract, characters 717–865 · show in text ↑
t0

“SUGARCANE”

✓ located verbatim in the source · title, characters 20–29 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Variety Isd37 gave the best callus induction at 4.0 mg/l 2,4-D.

Taxon
SUGARCANEt0
Material
Isd37e0
Treatment
4.0 mg/l of 2, 4-De0
Outcome
callus inductione0
Direction
Best resultse0

Not reportedComparator

Verbatim

e0

“Best results were observed from the varieties Isd36 and Isd37 at 4.0 mg/l of 2, 4-D and the variety Isd16 at 3.0 mg/l of 2, 4-D for callus induction”

✓ located verbatim in the source · abstract, characters 717–865 · show in text ↑
t0

“SUGARCANE”

✓ located verbatim in the source · title, characters 20–29 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Variety Isd16 gave the best callus induction at 3.0 mg/l 2,4-D.

Taxon
SUGARCANEt0
Material
Isd16e0
Treatment
3.0 mg/l of 2, 4-De0
Outcome
callus inductione0
Direction
Best resultse0

Not reportedComparator

Verbatim

e0

“Best results were observed from the varieties Isd36 and Isd37 at 4.0 mg/l of 2, 4-D and the variety Isd16 at 3.0 mg/l of 2, 4-D for callus induction”

✓ located verbatim in the source · abstract, characters 717–865 · show in text ↑
t0

“SUGARCANE”

✓ located verbatim in the source · title, characters 20–29 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with 1.0 mg/l BAP + 0.5 mg/l NAA gave the best shoot induction and multiplication.

Taxon
SUGARCANEt0
Treatment
1.0 mg/l BAP + 0.5 mg/l NAAe0
Outcome
induction and multiplication of shoote0
Direction
best resulte0

Not reportedComparator

Verbatim

e0

“1.0 mg/l BAP + 0.5 mg/l NAA showed the best result for induction and multiplication of shoot”

✓ located verbatim in the source · abstract, characters 992–1084 · show in text ↑
t0

“SUGARCANE”

✓ located verbatim in the source · title, characters 20–29 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NAA performed better than IBA for root initiation from proliferated shoots.

Taxon
SUGARCANEt0
Treatment
NAAe0
Outcome
root initiatione0
Direction
better performancee0
Comparator
IBAe0

Verbatim

e0

“NAA showed better performance than IBA for root initiation”

✓ located verbatim in the source · abstract, characters 1232–1290 · show in text ↑
t0

“SUGARCANE”

✓ located verbatim in the source · title, characters 20–29 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The best root formation was observed on MS medium with 5.0 mg/l NAA.

Taxon
SUGARCANEt0
Treatment
5.0 mg/l of NAAe0
Outcome
root formatione0
Direction
Best resulte0

Not reportedComparator

Verbatim

e0

“Best result of root formation was observed on MS medium supplemented with 5.0 mg/l of NAA”

✓ located verbatim in the source · abstract, characters 1292–1381 · show in text ↑
t0

“SUGARCANE”

✓ located verbatim in the source · title, characters 20–29 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Variety Isd37 produced the highest number of roots (13.47) at 5.0 mg/l NAA.

Taxon
SUGARCANEt0
Material
Isd37e0
Treatment
5.0 mg/l of NAAe0
Outcome
number of rootse0
Direction
higheste0
Value
13.47e0

Not reportedComparator

Verbatim

e0

“The variety Isd37 produced the highest number of roots (13.47) at 5.0 mg/l of NAA”

✓ located verbatim in the source · abstract, characters 1383–1464 · show in text ↑
t0

“SUGARCANE”

✓ located verbatim in the source · title, characters 20–29 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Plantlets were successfully transferred to soil with eighty to ninety percent survivability at normal temperature and 85% humidity.

Taxon
SUGARCANEt0
Outcome
survivabilitye0
Value
eighty to ninety percente0
Qualifier
normal temperature with 85% humiditye0

Verbatim

e0

“The plantlets were successfully transferred to soil with eighty to ninety percent survivability at normal temperature with 85% humidity”

✓ located verbatim in the source · abstract, characters 1466–1601 · show in text ↑
t0

“SUGARCANE”

✓ located verbatim in the source · title, characters 20–29 · show in text ↑

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.