Abstract
Indian economy gains 1.9% of National GDP by sugarcane cultivation. Plant growth hormones such as BAP, NAA, 2, 4-D, Kn, IBA were used to initiate cultures. A successful procedure was established in the culture conditions of variety 018. MS medium supplemented with 2,4-D (3.0 mg/l) , Kn (0.2 mg/l) and 3% sucrose was the best for the initiation of callusing from leaves and MS medium supplemented with BAP (1.0 mg/l), Kn (0.5mg/l) and 3% sucrose was best for shooting in eye bud from nodal region. The best multiplication rate of shooting in case of nodal eye bud was in MS medium supplemented with BAP(0.1 mg/l), Kn (0.5) & NAA (0.25 mg/L) and 3% sucrose where as for leaves MS medium supplemented with BAP (4.0 mg/l) , IBA (0.5 mg/L) and 3% sucrose. Root system was found in 1⁄2 MS medium with NAA (3.0 mg/l) and 6% sucrose. SATPAL SINGH BISHT Department of Biotechnology,Roland Institute of Pharmaceutical Sciences,Berhampur760010, Ganjam,Odisha.
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Claims 6
Claim · model-written summary
A propagation procedure was established for variety 018.
Not reportedComparator
Verbatim
e0“A successful procedure was established in the culture conditions of variety 018.”
t0“Sugarcane Variety 018”
Claim · model-written summary
MS medium with 2,4-D, Kn and sucrose was best for leaf callusing.
- Taxon
- sugarcanet0
- Material
- variety 018t0
- Organ / stage
- leavese0
- Treatment
- MS medium supplemented with 2,4-D (3.0 mg/l) , Kn (0.2 mg/l) and 3% sucrosee0
- Outcome
- initiation of callusing from leavese0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“MS medium supplemented with 2,4-D (3.0 mg/l) , Kn (0.2 mg/l) and 3% sucrose was the best for the initiation of callusing from leaves”
t0“Sugarcane Variety 018”
Claim · model-written summary
MS medium with BAP, Kn and sucrose was best for shoots from nodal eye buds.
- Taxon
- sugarcanet0
- Material
- variety 018t0
- Organ / stage
- eye bud from nodal regione0
- Treatment
- MS medium supplemented with BAP (1.0 mg/l), Kn (0.5mg/l) and 3% sucrosee0
- Outcome
- shooting in eye bud from nodal regione0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“MS medium supplemented with BAP (1.0 mg/l), Kn (0.5mg/l) and 3% sucrose was best for shooting in eye bud from nodal region”
t0“Sugarcane Variety 018”
Claim · model-written summary
A lower BAP medium with Kn and NAA gave the best nodal eye bud shoot multiplication.
- Taxon
- sugarcanet0
- Material
- variety 018t0
- Organ / stage
- nodal eye bude0
- Treatment
- MS medium supplemented with BAP(0.1 mg/l), Kn (0.5) & NAA (0.25 mg/L) and 3% sucrosee0
- Outcome
- multiplication rate of shootinge0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“best multiplication rate of shooting in case of nodal eye bud was in MS medium supplemented with BAP(0.1 mg/l), Kn (0.5) & NAA (0.25 mg/L) and 3% sucrose”
t0“Sugarcane Variety 018”
Claim · model-written summary
MS medium with BAP, IBA and sucrose gave the best multiplication from leaves.
- Taxon
- sugarcanet0
- Material
- variety 018t0
- Organ / stage
- leavese0
- Treatment
- MS medium supplemented with BAP (4.0 mg/l) , IBA (0.5 mg/L) and 3% sucrosee0
- Outcome
- leavese0
Not reportedComparatorDirection
Verbatim
e0“for leaves MS medium supplemented with BAP (4.0 mg/l) , IBA (0.5 mg/L) and 3% sucrose”
t0“Sugarcane Variety 018”
Claim · model-written summary
Half-strength MS medium with NAA and sucrose produced a root system.
- Taxon
- sugarcanet0
- Material
- variety 018t0
- Treatment
- 1⁄2 MS medium with NAA (3.0 mg/l) and 6% sucrosee0
- Outcome
- Root system was founde0
Not reportedDirection
Verbatim
e0“Root system was found in 1⁄2 MS medium with NAA (3.0 mg/l) and 6% sucrose.”
t0“Sugarcane Variety 018”
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