Abstract
Mesophyll K(+) retention ability has been recently reported as an important component of salinity stress tolerance in wheat. In order to investigate the role of ROS in regulating NaCl(-)induced K(+) efflux in wheat leaf mesophyll, a series of pharmacological experiments was conducted using MV (methyl viologen, superoxide radical inducer), DPI (an inhibitor of NADPH oxidase), H2O2 (to mimic apoplastic ROS), and EGCG ((-)-Epigallocatechin gallate, ROS scavenger). Mesophyll pre-treatment with 10 μM MV resulted in a significantly higher NaCl(-)induced K(+) efflux in leaf mesophyll, while 50 μM EGCG pre-treatment alleviated K(+) leakage under salt stress. No significant change in NaCl(-)induced K(+) efflux in leaf mesophyll was found in specimens pre-treated by H2O2 and DPI, compared with the control. The highest NaCl(-)induced H(+) efflux in leaf mesophyll was also found in samples pre-treated with MV, suggesting a futile cycle between increased H(+)-ATPase activity and ROS-induced K(+) leak. Overall, it is suggested that, under saline stress, K(+) efflux from wheat mesophyll is mediated predominantly by non-selective cation channels (NSCC) regulated by ROS produced in chloroplasts, at least in bread wheat.
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Claims 6
Claim · model-written summary
MV pretreatment increased salt-induced K efflux in wheat mesophyll.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- 10 μM MVe0
- Outcome
- NaCl(-)induced K(+) effluxe0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mesophyll pre-treatment with 10 μM MV resulted in a significantly higher NaCl(-)induced K(+) efflux in leaf mesophyll”
t0“wheat”
Claim · model-written summary
EGCG pretreatment alleviated potassium leakage under salt stress.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- 50 μM EGCG pre-treatmente0
- Outcome
- K(+) leakagee0
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“50 μM EGCG pre-treatment alleviated K(+) leakage under salt stress”
t0“wheat”
Claim · model-written summary
H2O2 pretreatment did not significantly change salt-induced potassium efflux versus control.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- H2O2e0
- Outcome
- NaCl(-)induced K(+) effluxe0
- Direction
- No significant changee0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant change in NaCl(-)induced K(+) efflux in leaf mesophyll was found in specimens pre-treated by H2O2 and DPI, compared with the control”
t0“wheat”
Claim · model-written summary
DPI pretreatment did not significantly change salt-induced potassium efflux versus control.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- DPIe0
- Outcome
- NaCl(-)induced K(+) effluxe0
- Direction
- No significant changee0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant change in NaCl(-)induced K(+) efflux in leaf mesophyll was found in specimens pre-treated by H2O2 and DPI, compared with the control”
t0“wheat”
Claim · model-written summary
MV pretreatment produced the highest salt-induced H efflux.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- MVe0
- Outcome
- NaCl(-)induced H(+) effluxe0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest NaCl(-)induced H(+) efflux in leaf mesophyll was also found in samples pre-treated with MV”
t0“wheat”
Claim · model-written summary
The authors suggest chloroplast ROS regulates NSCC-mediated K efflux under salinity.
- Taxon
- wheatTriticum aestivume0
- Outcome
- K(+) effluxe0
- Qualifier
- non-selective cation channels (NSCC) regulated by ROS produced in chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“K(+) efflux from wheat mesophyll is mediated predominantly by non-selective cation channels (NSCC) regulated by ROS produced in chloroplasts”
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