Abstract
The study included the use of tissue culture technique in rosemary (Rosmarinus officinalis L.) plant propagation starting from leaf explants and the production of callus. The first experiment consisted in the leaf explants sterilization using ten randomized replications of ethanol (C 2 H 5 OH), mercuric chloride (HgCl 2 ) and sodium hypochlorite (NaOCl) respectively, on six concentrations and five treatment periods, each. The results showed that the use of sodium hypochlorite in a concentration of 0.75% for a period of 15 minutes was the most effective by giving sterile explants after two weeks of culture. After the explants sterilization, the effect of six concentrations of benzyl adenine (BA) and six concentrations of naphthalene acetic acid (NAA) and the overlap between them on the callus formation were studied. Highest callus size (mm 3 ), callus fresh and dry weight (g) occurred at 0.2 mg/l BA and 1.5 mg/l of NAA.
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Claims 2
Claim · model-written summary
Sodium hypochlorite at 0.75% for 15 minutes most effectively sterilized rosemary leaf explants.
- Taxon
- rosemary
Salvia rosmarinuse0 - Organ / stage
- leafe2
- Treatment
- sodium hypochlorite in a concentration of 0.75% for a period of 15 minutese1
- Outcome
- sterile explantse1
- Direction
- most effectivee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rosemary (Rosmarinus officinalis L.)”
e1“the use of sodium hypochlorite in a concentration of 0.75% for a period of 15 minutes was the most effective by giving sterile explants after two weeks of culture.”
e2“starting from leaf explants”
Claim · model-written summary
BA at 0.2 mg/l with NAA at 1.5 mg/l produced the highest rosemary callus size and fresh and dry weight.
- Taxon
- rosemarySalvia rosmarinuse0
- Organ / stage
- calluse1
- Treatment
- 0.2 mg/l BA and 1.5 mg/l of NAAe1
- Outcome
- callus size (mm 3 ), callus fresh and dry weight (g)e1
- Direction
- Higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rosemary (Rosmarinus officinalis L.)”
e1“Highest callus size (mm 3 ), callus fresh and dry weight (g) occurred at 0.2 mg/l BA and 1.5 mg/l of NAA.”
The same questions in other papers
N-benzyladenine · growth · Salvia rosmarinus (rosemary)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
1-naphthaleneacetic acid · growth · Salvia rosmarinus (rosemary)Results agree
7 papers in the same direction as this paper
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.