Abstract
Abstract The study carried out in plant tissue culture laboratory at Department of Biology - College of Science - University of Al-Qadisiyah, included several experiments on the effect of different growth regulators concentration of auxins and cytokinins on the growth and characteristics of callus of Rosmarinus officinalis L. growing under light and dark conditions. Explant ( tops and the lateral buds with a length of 0.5-1.0 cm) sterilized with ethanol solution and sodium hypochlorate, then growing on MS medium with added different concentrations of growth regulators and incubated in the growth chamber under the influence of lighting factor (light and dark) at a temperature of 25 °C, the data were taken every week and after 45 days of cultivation that. The best combination 2,4-D 1.0 mg/l with BA 0.5 mg/l that gave the largest callus weight in the light 3.75 gm, while in the dark, the best combination 1.0 mg/l NAA and 0.5 mg/l BA gave the largest callus weight 3.60 gm, same combination which gave the highest percentage of callus emergence after 2 weeks was 100% in the dark and 95% in the light. The texture, color and shape of the callus varied as the combination
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Claims 4
Claim · model-written summary
Under light, 2,4-D with BA yielded the greatest rosemary callus weight, 3.75 gm.
- Taxon
- Rosmarinus officinalis L.
Salvia rosmarinust0 - Treatment
- 2,4-D 1.0 mg/l with BA 0.5 mg/le0
- Outcome
- callus weighte0
- Direction
- largeste0
- Value
- 3.75 gme0
- Setting
- lighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best combination 2,4-D 1.0 mg/l with BA 0.5 mg/l that gave the largest callus weight in the light 3.75 gm”
t0“Rosmarinus officinalis L.”
Claim · model-written summary
In darkness, NAA with BA yielded the greatest rosemary callus weight, 3.60 gm.
- Taxon
- Rosmarinus officinalis L.Salvia rosmarinust0
- Treatment
- 1.0 mg/l NAA and 0.5 mg/l BAe0
- Outcome
- callus weighte0
- Direction
- largeste0
- Value
- 3.60 gme0
- Setting
- darke0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while in the dark, the best combination 1.0 mg/l NAA and 0.5 mg/l BA gave the largest callus weight 3.60 gm”
t0“Rosmarinus officinalis L.”
Claim · model-written summary
NAA with BA gave maximum rosemary callus emergence after two weeks, 100% in darkness and 95% in light.
- Taxon
- Rosmarinus officinalis L.Salvia rosmarinust0
- Treatment
- 1.0 mg/l NAA and 0.5 mg/l BAe1
- Outcome
- percentage of callus emergencee0
- Direction
- higheste0
- Value
- 100%e0
- Qualifier
- after 2 weekse0
- Setting
- darke0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“same combination which gave the highest percentage of callus emergence after 2 weeks was 100% in the dark and 95% in the light”
t0“Rosmarinus officinalis L.”
e1“1.0 mg/l NAA and 0.5 mg/l BA gave the largest callus weight 3.60 gm”
Claim · model-written summary
Rosemary callus texture, color and shape varied by regulator combination.
- Taxon
- Rosmarinus officinalis L.Salvia rosmarinust0
- Treatment
- combinatione0
- Outcome
- texture, color and shape of the calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The texture, color and shape of the callus varied as the combination”
t0“Rosmarinus officinalis L.”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.