Abstract
Apical bud explant measuring 1.0 cm was excised from in vitro plant of Rosmarinus officinalis and cultured on MSBM fortified with cytokinin BAP (8.88µM) and auxins in different concentrations of IAA (2.85 µM, 5.70 µM, 8.5 5 µM, 11.40 µM, 14.25 µM) and 2,4-D (2.26 µM, 4.52 µM, 6.78 µM, 9.04 µM, 11.30 µM) respectively in order to induce callus MSBM + BAP (8.88 µM) + IAA (5.70 µM) was found to be the best medium for the induction and growth of whitish green compact callus from the apical bud explants of Rosmarinus officinalis when compared to the callus derived from MSBM + BAP (8.88 µM) + 2,4-D (4.52 µM). It was observed in the present study that MSBM and BAP (8.88 μM) with IAA (2.85 μM) was the most suitable medium for initiation shoots and multiple shoot formation from in vitro apical bud of R. officinalis. These axenic plants were hardened and successfully established in the field with 80% survival frequency.
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Claims 3
Claim · model-written summary
MSBM + BAP + IAA (5.70 µM) was the best medium for callus induction and growth from Rosmarinus officinalis apical bud explants compared with MSBM + BAP + 2,4-D (4.52 µM).
- Taxon
- Rosmarinus officinalis
Salvia rosmarinuse0 - Organ / stage
- apical bud explantse0
- Treatment
- MSBM + BAP (8.88 µM) + IAA (5.70 µM)e0
- Outcome
- induction and growth of whitish green compact calluse0
- Direction
- beste0
- Comparator
- MSBM + BAP (8.88 µM) + 2,4-D (4.52 µM)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSBM + BAP (8.88 µM) + IAA (5.70 µM) was found to be the best medium for the induction and growth of whitish green compact callus from the apical bud explants of Rosmarinus officinalis when compared to the callus derived from MSBM + BAP (8.88 µM) + 2,4-D (4.52 µM)”
Claim · model-written summary
MSBM with BAP (8.88 μM) and IAA (2.85 μM) was the most suitable medium for shoot initiation and multiple shoot formation from in vitro apical buds of R. officinalis.
- Taxon
- R. officinalisSalvia rosmarinuse0
- Organ / stage
- apical bude0
- Treatment
- MSBM and BAP (8.88 μM) with IAA (2.85 μM)e0
- Outcome
- initiation shoots and multiple shoot formatione0
- Direction
- most suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSBM and BAP (8.88 μM) with IAA (2.85 μM) was the most suitable medium for initiation shoots and multiple shoot formation from in vitro apical bud of R. officinalis”
Claim · model-written summary
Axenic plantlets were hardened and established in the field with 80% survival.
- Taxon
- ROSMARINUS OFFICINALISSalvia rosmarinust0
- Outcome
- survival frequencye0
- Value
- 80%e0
- Setting
- in the fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“ROSMARINUS OFFICINALIS L.”
e0“These axenic plants were hardened and successfully established in the field with 80% survival frequency”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.