Paper · 2015 · Environmental Nanotechnology Monitoring & Management

Phytotoxicity of River Chenab sediments: In vitro morphological and biochemical response of Brassica napus L.

Attarad Ali; Abdul Rehman Phull; Muhammad Zia; et al.

9
claims
17
verbatim quotes
22
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

River Chenab is one of the major sources of water for irrigation of agriculture fields in Punjab, Pakistan. The present investigation was conducted to appraise the effect of river Chenab sediments on growth; morphological and biochemical prospects of in vitro grown Brassica napus seedling. A total 19 residue samples collected from different sites of River Chenab were evaluated. The sediments extracts, in most of the cases, significantly influenced on final germination, rate of germination and mean period of final germination of B. napus seedlings in comparison with controls. Reduction in root length was observed as compared with shoot length. Decrease in relative dry weight of seedlings ranging from 45% to 64% was also examined. Biochemical analysis revealed that the sediments extracts tend to increase in total protein and total phenolic contents in B. napus plants while variation in MDA and flavonoid contents were observed as compared with control. Increase in chlorophyll a & b and carotenoid contents were also observed in plants germinated in the presence of sediments extracts except sample 4. The enzymes (POD, SOD and protease); responsible to mitigate hazardous effects of sediment contamination; were found elevated in the seedlings. Phytotoxic evaluations of sediments demonstrate that it is consistent and practical tool for assessing quality of sediment. However, increased activities of antioxidants; enzymes and proteins favor the adaptation or tolerance to contamination by the seedlings.

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Claims 9

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Sediment extracts significantly affected final germination, rate of germination and mean period of final germination of Brassica napus seedlings compared with controls.

Taxon
B. napusBrassica napuse1
Treatment
sediments extractse1
Outcome
final germination, rate of germination and mean period of final germinatione1
Comparator
controlse1
Setting
In vitrot0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The sediments extracts, in most of the cases, significantly influenced on final germination, rate of germination and mean period of final germination of B. napus seedlings in comparison with controls”

✓ located verbatim in the source · abstract, characters 381–580 · show in text ↑
t0

“In vitro morphological and biochemical response of Brassica napus L.”

✓ located verbatim in the source · title, characters 41–109 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Root length of Brassica napus seedlings was reduced compared with shoot length under sediment extract treatment.

Taxon
B. napusBrassica napuse1
Treatment
sediments extractse1
Outcome
root lengthe2
Direction
Reductione2
Comparator
shoot lengthe2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The sediments extracts, in most of the cases, significantly influenced on final germination, rate of germination and mean period of final germination of B. napus seedlings in comparison with controls”

✓ located verbatim in the source · abstract, characters 381–580 · show in text ↑
e2

“Reduction in root length was observed as compared with shoot length”

✓ located verbatim in the source · abstract, characters 582–649 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Relative dry weight of Brassica napus seedlings decreased by 45% to 64% under sediment extract treatment.

Taxon
B. napusBrassica napuse1
Treatment
sediments extractse1
Outcome
relative dry weight of seedlingse3
Direction
Decreasee3
Value
45% to 64%e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The sediments extracts, in most of the cases, significantly influenced on final germination, rate of germination and mean period of final germination of B. napus seedlings in comparison with controls”

✓ located verbatim in the source · abstract, characters 381–580 · show in text ↑
e3

“Decrease in relative dry weight of seedlings ranging from 45% to 64% was also examined”

✓ located verbatim in the source · abstract, characters 651–737 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Sediment extracts tended to increase total protein and total phenolic contents in Brassica napus plants.

Taxon
B. napusBrassica napuse4
Treatment
sediments extractse4
Outcome
total protein and total phenolic contentse4
Direction
increasee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e4

“Biochemical analysis revealed that the sediments extracts tend to increase in total protein and total phenolic contents in B. napus plants”

✓ located verbatim in the source · abstract, characters 739–877 · show in text ↑
Associationobservedoriginal resultmixedmeasured

Claim · model-written summary

MDA and flavonoid contents of Brassica napus varied compared with control.

Taxon
B. napusBrassica napuse4
Outcome
MDA and flavonoid contentse5
Comparator
controle5

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e4

“Biochemical analysis revealed that the sediments extracts tend to increase in total protein and total phenolic contents in B. napus plants”

✓ located verbatim in the source · abstract, characters 739–877 · show in text ↑
e5

“while variation in MDA and flavonoid contents were observed as compared with control”

✓ located verbatim in the source · abstract, characters 878–962 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Chlorophyll a & b and carotenoid contents increased in Brassica napus plants germinated in the presence of sediment extracts, except sample 4.

Taxon
B. napusBrassica napuse4
Treatment
presence of sediments extractse6
Outcome
chlorophyll a & b and carotenoid contentse6
Direction
Increasee6
Qualifier
except sample 4e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e4

“Biochemical analysis revealed that the sediments extracts tend to increase in total protein and total phenolic contents in B. napus plants”

✓ located verbatim in the source · abstract, characters 739–877 · show in text ↑
e6

“Increase in chlorophyll a & b and carotenoid contents were also observed in plants germinated in the presence of sediments extracts except sample 4”

✓ located verbatim in the source · abstract, characters 964–1111 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Antioxidant enzymes POD, SOD and protease were elevated in Brassica napus seedlings.

Taxon
Brassica napusBrassica napust0
Gene
POD, SOD and proteasee7
Outcome
enzymese7
Direction
elevatede7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 92–109 · show in text ↑
e7

“The enzymes (POD, SOD and protease); responsible to mitigate hazardous effects of sediment contamination; were found elevated in the seedlings”

✓ located verbatim in the source · abstract, characters 1113–1255 · show in text ↑
Method / resourceauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Phytotoxicity evaluation of sediments is a consistent and practical tool for assessing sediment quality.

Taxon
Brassica napusBrassica napust0
Outcome
assessing quality of sedimente7
Qualifier
consistent and practical toole7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 92–109 · show in text ↑
e7

“Phytotoxic evaluations of sediments demonstrate that it is consistent and practical tool for assessing quality of sediment”

✓ located verbatim in the source · abstract, characters 1257–1379 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Increased activities of antioxidants, enzymes and proteins favour adaptation or tolerance of Brassica napus seedlings to sediment contamination.

Taxon
Brassica napusBrassica napust0
Outcome
adaptation or tolerance to contaminatione7
Qualifier
increased activities of antioxidants; enzymes and proteinse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Brassica napus L.”

✓ located verbatim in the source · title, characters 92–109 · show in text ↑
e7

“increased activities of antioxidants; enzymes and proteins favor the adaptation or tolerance to contamination by the seedlings”

✓ located verbatim in the source · abstract, characters 1390–1516 · show in text ↑

botanical · growth · Brassica napus (oilseed rape)Positive and negative results

65 papers: 22 positive, 43 negative or no effect

42 papers in the same direction as this paper · 22 in the opposite direction

  1. positive
  2. positive
  3. positive

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.