Abstract
Isolated winter wheat chromatin can synthesize heterogenous RNA with endogenous RNA polymerase. RNA synthesized with α-amanitin-sensitive RNA polymerase changed, quantitatively and qualitatively, during periods of germination at 24°C and cold treatment at 4°C: (1) synthesis of medium (9 to 20S) mRNA at 24°C decreased while that of small (5 to 9S) mRNA increased in the later stages of germination, and (2) synthesis of medium mRNA at 3°C decreased while that of small mRNA increased during cold treatment. Chromatin from germinated shoots predominantly synthesized medium mRNA at 24°C and small mRNA at 3°C. Chromatin from cold-treated shoots predominantly synthesized large (more than 20S) and medium mRNAs at 24°C and small mRNA at 3°C. These results suggest that the ability to synthesize high molecular weight RNA at 24°C is markedly stimulated by cold treatment of young shoots, and that some of the high molecular weight transcripts activated at 24°C have an important role in vernalized wheat shoots transferred from 4°C to 24°C for further development.
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Claims 9
Claim · model-written summary
Winter wheat chromatin synthesized heterogeneous RNA using endogenous polymerase.
- Taxon
- winter wheat
Triticum aestivume0 - Gene
- endogenous RNA polymerasee0
- Outcome
- heterogenous RNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Isolated winter wheat chromatin can synthesize heterogenous RNA with endogenous RNA polymerase.”
Claim · model-written summary
Medium mRNA synthesis decreased at 24°C late in germination.
- Taxon
- winter wheat
Triticum aestivumt0 - Outcome
- synthesis of medium (9 to 20S) mRNAe0
- Direction
- decreasede0
- Qualifier
- later stages of germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“synthesis of medium (9 to 20S) mRNA at 24°C decreased while that of small (5 to 9S) mRNA increased in the later stages of germination”
t0“winter wheat”
Claim · model-written summary
Small mRNA synthesis increased at 24°C late in germination.
- Taxon
- winter wheat
Triticum aestivumt0 - Outcome
- small (5 to 9S) mRNAe0
- Direction
- increasede0
- Qualifier
- later stages of germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“synthesis of medium (9 to 20S) mRNA at 24°C decreased while that of small (5 to 9S) mRNA increased in the later stages of germination”
t0“winter wheat”
Claim · model-written summary
Medium mRNA synthesis decreased at 3°C during cold treatment.
- Taxon
- winter wheat
Triticum aestivumt0 - Treatment
- cold treatmente0
- Outcome
- synthesis of medium mRNAe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“synthesis of medium mRNA at 3°C decreased while that of small mRNA increased during cold treatment.”
t0“winter wheat”
Claim · model-written summary
Small mRNA synthesis increased at 3°C during cold treatment.
- Taxon
- winter wheat
Triticum aestivumt0 - Treatment
- cold treatmente0
- Outcome
- small mRNAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“synthesis of medium mRNA at 3°C decreased while that of small mRNA increased during cold treatment.”
t0“winter wheat”
Claim · model-written summary
Chromatin from germinated shoots predominantly synthesized medium mRNA at 24°C and small mRNA at 3°C.
- Taxon
- winter wheat
Triticum aestivumt0 - Organ / stage
- germinated shootse0
- Outcome
- predominantly synthesized medium mRNA at 24°C and small mRNA at 3°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Chromatin from germinated shoots predominantly synthesized medium mRNA at 24°C and small mRNA at 3°C.”
t0“winter wheat”
Claim · model-written summary
Chromatin from cold-treated shoots predominantly synthesized large and medium mRNAs at 24°C, and small mRNA at 3°C.
- Taxon
- winter wheat
Triticum aestivumt0 - Organ / stage
- cold-treated shootse0
- Outcome
- predominantly synthesized large (more than 20S) and medium mRNAs at 24°C and small mRNA at 3°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Chromatin from cold-treated shoots predominantly synthesized large (more than 20S) and medium mRNAs at 24°C and small mRNA at 3°C.”
t0“winter wheat”
Claim · model-written summary
Cold treatment was inferred to stimulate high molecular weight RNA synthesis at 24°C.
- Taxon
- winter wheat
Triticum aestivumt0 - Organ / stage
- young shootse0
- Treatment
- cold treatmente0
- Outcome
- ability to synthesize high molecular weight RNAe0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the ability to synthesize high molecular weight RNA at 24°C is markedly stimulated by cold treatment of young shoots”
t0“winter wheat”
Claim · model-written summary
The authors inferred a developmental role for high molecular weight transcripts in vernalized shoots.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- vernalized wheat shootse0
- Outcome
- further developmente0
- Qualifier
- high molecular weight transcripts activated at 24°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“some of the high molecular weight transcripts activated at 24°C have an important role in vernalized wheat shoots transferred from 4°C to 24°C for further development.”