Paper · 1979 · Plant and Cell Physiology

Ribonucleic acid synthesis at low and high temperatures in isolated winter wheat chromatin

Tadashi Tazawa; Koh Ishikawa; Kimiko Sasaki

9
claims
16
verbatim quotes
1
citations
3
research areas
—
Not open access

Abstract

Isolated winter wheat chromatin can synthesize heterogenous RNA with endogenous RNA polymerase. RNA synthesized with α-amanitin-sensitive RNA polymerase changed, quantitatively and qualitatively, during periods of germination at 24°C and cold treatment at 4°C: (1) synthesis of medium (9 to 20S) mRNA at 24°C decreased while that of small (5 to 9S) mRNA increased in the later stages of germination, and (2) synthesis of medium mRNA at 3°C decreased while that of small mRNA increased during cold treatment. Chromatin from germinated shoots predominantly synthesized medium mRNA at 24°C and small mRNA at 3°C. Chromatin from cold-treated shoots predominantly synthesized large (more than 20S) and medium mRNAs at 24°C and small mRNA at 3°C. These results suggest that the ability to synthesize high molecular weight RNA at 24°C is markedly stimulated by cold treatment of young shoots, and that some of the high molecular weight transcripts activated at 24°C have an important role in vernalized wheat shoots transferred from 4°C to 24°C for further development.

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Claims 9

Observationobservedoriginal resultmeasured

Claim · model-written summary

Winter wheat chromatin synthesized heterogeneous RNA using endogenous polymerase.

Taxon
winter wheatTriticum aestivume0
Gene
endogenous RNA polymerasee0
Outcome
heterogenous RNAe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Isolated winter wheat chromatin can synthesize heterogenous RNA with endogenous RNA polymerase.”

✓ located verbatim in the source · abstract, characters 0–95 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Medium mRNA synthesis decreased at 24°C late in germination.

Taxon
winter wheatTriticum aestivumt0
Outcome
synthesis of medium (9 to 20S) mRNAe0
Direction
decreasede0
Qualifier
later stages of germinatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“synthesis of medium (9 to 20S) mRNA at 24°C decreased while that of small (5 to 9S) mRNA increased in the later stages of germination”

✓ located verbatim in the source · abstract, characters 265–398 · show in text ↑
t0

“winter wheat”

✓ located verbatim in the source · title, characters 68–80 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Small mRNA synthesis increased at 24°C late in germination.

Taxon
winter wheatTriticum aestivumt0
Outcome
small (5 to 9S) mRNAe0
Direction
increasede0
Qualifier
later stages of germinatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“synthesis of medium (9 to 20S) mRNA at 24°C decreased while that of small (5 to 9S) mRNA increased in the later stages of germination”

✓ located verbatim in the source · abstract, characters 265–398 · show in text ↑
t0

“winter wheat”

✓ located verbatim in the source · title, characters 68–80 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Medium mRNA synthesis decreased at 3°C during cold treatment.

Taxon
winter wheatTriticum aestivumt0
Treatment
cold treatmente0
Outcome
synthesis of medium mRNAe0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“synthesis of medium mRNA at 3°C decreased while that of small mRNA increased during cold treatment.”

✓ located verbatim in the source · abstract, characters 408–507 · show in text ↑
t0

“winter wheat”

✓ located verbatim in the source · title, characters 68–80 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Small mRNA synthesis increased at 3°C during cold treatment.

Taxon
winter wheatTriticum aestivumt0
Treatment
cold treatmente0
Outcome
small mRNAe0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“synthesis of medium mRNA at 3°C decreased while that of small mRNA increased during cold treatment.”

✓ located verbatim in the source · abstract, characters 408–507 · show in text ↑
t0

“winter wheat”

✓ located verbatim in the source · title, characters 68–80 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Chromatin from germinated shoots predominantly synthesized medium mRNA at 24°C and small mRNA at 3°C.

Taxon
winter wheatTriticum aestivumt0
Organ / stage
germinated shootse0
Outcome
predominantly synthesized medium mRNA at 24°C and small mRNA at 3°Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Chromatin from germinated shoots predominantly synthesized medium mRNA at 24°C and small mRNA at 3°C.”

✓ located verbatim in the source · abstract, characters 508–609 · show in text ↑
t0

“winter wheat”

✓ located verbatim in the source · title, characters 68–80 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Chromatin from cold-treated shoots predominantly synthesized large and medium mRNAs at 24°C, and small mRNA at 3°C.

Taxon
winter wheatTriticum aestivumt0
Organ / stage
cold-treated shootse0
Outcome
predominantly synthesized large (more than 20S) and medium mRNAs at 24°C and small mRNA at 3°Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Chromatin from cold-treated shoots predominantly synthesized large (more than 20S) and medium mRNAs at 24°C and small mRNA at 3°C.”

✓ located verbatim in the source · abstract, characters 610–740 · show in text ↑
t0

“winter wheat”

✓ located verbatim in the source · title, characters 68–80 · show in text ↑
Intervention effectauthor interpretationoriginal resultmeasured

Claim · model-written summary

Cold treatment was inferred to stimulate high molecular weight RNA synthesis at 24°C.

Taxon
winter wheatTriticum aestivumt0
Organ / stage
young shootse0
Treatment
cold treatmente0
Outcome
ability to synthesize high molecular weight RNAe0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the ability to synthesize high molecular weight RNA at 24°C is markedly stimulated by cold treatment of young shoots”

✓ located verbatim in the source · abstract, characters 768–884 · show in text ↑
t0

“winter wheat”

✓ located verbatim in the source · title, characters 68–80 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors inferred a developmental role for high molecular weight transcripts in vernalized shoots.

Organ / stage
vernalized wheat shootse0
Outcome
further developmente0
Qualifier
high molecular weight transcripts activated at 24°Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“some of the high molecular weight transcripts activated at 24°C have an important role in vernalized wheat shoots transferred from 4°C to 24°C for further development.”

✓ located verbatim in the source · abstract, characters 895–1062 · show in text ↑