Abstract
Although hybrid proline-rich proteins (HyPRPs) are ubiquitous in plants, little is known about their roles other than as cell-wall structural proteins. We identified the gene HyPRP1 in Capsicum annuum and Nicotiana benthamiana, which encodes a protein containing proline-rich domain and eight-cysteine motif (8CM) that is constitutively expressed in various organs, mostly in the root, but is down-regulated upon inoculation with either incompatible or compatible pathogens. Ectopic expression of HyPRP1 in plants accelerated cell death, showing developmental abnormality with down-regulation of ROS-scavenging genes, and enhanced pathogen susceptibility suppressing expression of defense-related genes. Conversely, silencing of HyPRP1 suppressed pathogen-induced cell death, but enhanced disease resistance, with up-regulation of defense-related genes and inhibition of in planta growth of bacterial pathogens independently of signal molecule-mediated pathways. Furthermore, the secreted 8CM was sufficient for these HyPRP1 functions. Together, our results suggest that a common plant cell-wall structural protein, HyPRP1, performs distinct dual roles in positive regulation of cell death and negative regulation of basal defense against pathogen.
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Claims 13
Claim · model-written summary
HyPRP1 encodes a protein with a proline-rich domain and eight-cysteine motif.
- Taxon
- Capsicum annuum
Capsicum annuume0 - Gene
- HyPRP1e0
- Outcome
- protein containing proline-rich domain and eight-cysteine motif (8CM)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified the gene HyPRP1 in Capsicum annuum and Nicotiana benthamiana, which encodes a protein containing proline-rich domain and eight-cysteine motif (8CM)”
Claim · model-written summary
HyPRP1 is constitutively expressed across organs, predominantly in roots.
- Taxon
- Capsicum annuumCapsicum annuume1
- Organ / stage
- roote0
- Gene
- HyPRP1t0
- Outcome
- constitutively expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“that is constitutively expressed in various organs, mostly in the root”
t0“A common plant cell‐wall protein HyPRP1”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
HyPRP1 expression declines after inoculation with compatible or incompatible pathogens.
- Taxon
- Capsicum annuumCapsicum annuume1
- Treatment
- inoculation with either incompatible or compatible pathogense0
- Interactor
- pathogense0pathogen
- Gene
- HyPRP1t0
- Outcome
- HyPRP1t0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is down-regulated upon inoculation with either incompatible or compatible pathogens.”
t0“A common plant cell‐wall protein HyPRP1”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
Ectopic HyPRP1 expression accelerated cell death.
- Taxon
- plantsViridiplantaee0
- Treatment
- Ectopic expression of HyPRP1e0
- Gene
- HyPRP1e0
- Outcome
- cell deathe0
- Direction
- acceleratede0
Not reportedComparator
Verbatim
e0“Ectopic expression of HyPRP1 in plants accelerated cell death”
Claim · model-written summary
Ectopic HyPRP1 expression caused developmental abnormality.
- Taxon
- plantsViridiplantaee0
- Treatment
- Ectopic expression of HyPRP1e0
- Gene
- HyPRP1e0
- Outcome
- developmental abnormalitye0
Not reportedComparatorDirection
Verbatim
e0“Ectopic expression of HyPRP1 in plants accelerated cell death, showing developmental abnormality”
Claim · model-written summary
Ectopic HyPRP1 expression lowered ROS-scavenging gene expression.
- Taxon
- plantsViridiplantaee2
- Treatment
- Ectopic expression of HyPRP1e2
- Gene
- ROS-scavenging genese0
- Outcome
- ROS-scavenging genese0
- Direction
- down-regulatione0
Not reportedComparator
Verbatim
e0“showing developmental abnormality with down-regulation of ROS-scavenging genes”
t0“A common plant cell‐wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
Ectopic HyPRP1 expression increased pathogen susceptibility.
- Taxon
- plantsViridiplantaee2
- Treatment
- Ectopic expression of HyPRP1e2
- Interactor
- pathogene0pathogen
- Outcome
- pathogen susceptibilitye0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“enhanced pathogen susceptibility suppressing expression of defense-related genes.”
t0“A common plant cell‐wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
Ectopic HyPRP1 expression suppressed defense-related gene expression.
- Taxon
- plantsViridiplantaee2
- Treatment
- Ectopic expression of HyPRP1e2
- Gene
- defense-related genese0
- Outcome
- expression of defense-related genese0
- Direction
- suppressinge0
Not reportedComparator
Verbatim
e0“enhanced pathogen susceptibility suppressing expression of defense-related genes.”
t0“A common plant cell‐wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
HyPRP1 silencing suppressed pathogen-induced cell death.
- Taxon
- plantsViridiplantaee2
- Treatment
- silencing of HyPRP1e0
- Interactor
- pathogene0pathogen
- Gene
- HyPRP1e0
- Outcome
- pathogen-induced cell deathe0
- Direction
- suppressede0
Not reportedComparator
Verbatim
e0“silencing of HyPRP1 suppressed pathogen-induced cell death”
t0“A common plant cell‐wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
HyPRP1 silencing enhanced disease resistance.
- Taxon
- plantsViridiplantaee2
- Treatment
- silencing of HyPRP1e0
- Interactor
- pathogenst0pathogen
- Gene
- HyPRP1e0
- Outcome
- disease resistancee0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“silencing of HyPRP1 suppressed pathogen-induced cell death, but enhanced disease resistance”
t0“A common plant cell‐wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
HyPRP1 silencing upregulated defense-related genes.
- Taxon
- plantsViridiplantaee2
- Treatment
- silencing of HyPRP1e3
- Gene
- defense-related genese0
- Outcome
- defense-related genese0
- Direction
- up-regulatione0
Not reportedComparator
Verbatim
e0“with up-regulation of defense-related genes”
t0“A common plant cell‐wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
HyPRP1 silencing inhibited bacterial pathogen growth in plants.
- Taxon
- plantsViridiplantaee2
- Treatment
- silencing of HyPRP1e3
- Interactor
- bacterial pathogense0pathogen
- Outcome
- in planta growth of bacterial pathogense0
- Direction
- inhibitione0
- Qualifier
- independently of signal molecule-mediated pathwayse0
Not reportedComparator
Verbatim
e0“inhibition of in planta growth of bacterial pathogens independently of signal molecule-mediated pathways.”
t0“A common plant cell‐wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
Claim · model-written summary
The secreted 8CM motif sufficed for the reported HyPRP1 functions.
- Taxon
- plantsViridiplantaee2
- Gene
- 8CMe0
- Outcome
- HyPRP1 functionse0
- Qualifier
- secretede0
Verbatim
e0“the secreted 8CM was sufficient for these HyPRP1 functions.”
t0“A common plant cell‐wall protein HyPRP1 has dual roles as a positive regulator of cell death and a negative regulator of basal defense against pathogens”
e1“Capsicum annuum”
e2“Ectopic expression of HyPRP1 in plants”
e3“silencing of HyPRP1”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.