Abstract
Antagonism against the grey mould pathogen Botrytis cinerea by Pseudomonas antimicrobica was demonstrated in vitro and in vivo. Cell-free filtrates showed activity against B. cinerea growing on Potato Dextrose Agar (PDA) in a media-dependent manner with the most distinct antagonism being produced in Czapek Dox Broth (CDB). Cell-free filtrates of CDB-grown cultures also significantly reduced conidial germination of B. cinerea. An assay based on the inhibition of conidial germination was compared with two assays measuring the antagonism of mycelial growth on PDA. The conidial germination bioassay was more sensitive in the detection of this antifungal activity than the Petri dish bioassay while a bioassay using Microdetection plates did not detect antagonism due to the small loading capacity of the latter. The conidial germination bioassay was modified for detection of antibiosis on the surface of strawberry leaves. Significant reductions in percentage conidial germination were recorded on the surface of leaves of both micropropagated and glasshouse grown strawberry plants when the antifungal compounds of Ps. antimicrobica were applied to the leaf tissue with the conidia. In addition, antifungal compounds were also detectable when conidia were applied to leaf tissue which had previously been sprayed with cells of Ps. antimicrobica. These tests indicate that Ps. antimicrobica would be a suitable biocontrol agent for the control of B. cinerea.
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Claims 8
Claim · model-written summary
Pseudomonas antagonized Botrytis in vitro and in vivo.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae1 - Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Second interactor
- Pseudomonas antimicrobica
Burkholderia gladiolie0biocontrol_agent - Outcome
- Antagonism against the grey mould pathogen Botrytis cinereae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Antagonism against the grey mould pathogen Botrytis cinerea by Pseudomonas antimicrobica was demonstrated in vitro and in vivo”
e1“strawberry leaves”
Claim · model-written summary
Cell-free filtrates antagonized Botrytis on PDA, most clearly when grown in CDB.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- Cell-free filtratese0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- activity against B. cinereae0
- Direction
- most distinct antagonisme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell-free filtrates showed activity against B. cinerea growing on Potato Dextrose Agar (PDA) in a media-dependent manner with the most distinct antagonism being produced in Czapek Dox Broth (CDB)”
e1“strawberry leaves”
Claim · model-written summary
CDB-grown cell-free filtrates significantly reduced Botrytis conidial germination.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- Cell-free filtrates of CDB-grown culturese0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- conidial germination of B. cinereae0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell-free filtrates of CDB-grown cultures also significantly reduced conidial germination of B. cinerea”
e1“strawberry leaves”
Claim · model-written summary
Conidial germination bioassay was more sensitive than Petri dish assay.
- Taxon
- strawberryFragaria ×ananassae1
- Outcome
- sensitive in the detection of this antifungal activitye0
- Direction
- more sensitivee0
- Comparator
- Petri dish bioassaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The conidial germination bioassay was more sensitive in the detection of this antifungal activity than the Petri dish bioassay”
e1“strawberry leaves”
Claim · model-written summary
Microdetection plate bioassay did not detect antagonism because of small loading capacity.
- Taxon
- strawberryFragaria ×ananassae1
- Outcome
- did not detect antagonisme0
- Qualifier
- small loading capacitye0
- Comparator
- Microdetection platese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a bioassay using Microdetection plates did not detect antagonism due to the small loading capacity of the latter”
e1“strawberry leaves”
Claim · model-written summary
Applied antifungal compounds significantly reduced Botrytis conidial germination on strawberry leaves.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- antifungal compounds of Ps. antimicrobicae0
- Interactor
- conidiae0pathogen
- Second interactor
- Ps. antimicrobicae0biocontrol_agent
- Outcome
- percentage conidial germinatione0
- Direction
- reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant reductions in percentage conidial germination were recorded on the surface of leaves of both micropropagated and glasshouse grown strawberry plants when the antifungal compounds of Ps. antimicrobica were applied to the leaf tissue with the conidia”
Claim · model-written summary
Antifungal compounds remained detectable when conidia were applied after leaf spraying with Pseudomonas cells.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- sprayed with cells of Ps. antimicrobicae0
- Interactor
- Ps. antimicrobicae0biocontrol_agent
- Outcome
- antifungal compounds were also detectablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antifungal compounds were also detectable when conidia were applied to leaf tissue which had previously been sprayed with cells of Ps. antimicrobica”
e1“strawberry leaves”
Claim · model-written summary
Authors judged Pseudomonas suitable for Botrytis biocontrol.
- Taxon
- strawberryFragaria ×ananassae1
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Second interactor
- Ps. antimicrobicae0biocontrol_agent
- Outcome
- control of B. cinereae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ps. antimicrobica would be a suitable biocontrol agent for the control of B. cinerea”
e1“strawberry leaves”
The same questions in other papers
pathogen product · Botrytis cinerea · Fragaria x ananassa (strawberry)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →fungicide · disease · Fragaria x ananassa (strawberry)Positive and negative results
18 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- no effect
18 papers more in this group
See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.