Abstract
Bipolaris sorokiniana causes spot blotch, an important disease of wheat in Asia and America. To understand the host response to challenge by B. sorokiniana, we calculated the plant disease index (PDI) of twelve popular wheat (Triticum aestivum L.) genotypes and examined the expression levels of PR protein genes and wax synthesizing genes on an inoculation time-course using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Gene expression was studied in a resistant (R) and a highly susceptible (HS) genotype selected from a PDI experiment under glasshouse conditions. A gene (β-1,3-glucanase II) was induced at 24 h post inoculation and to higher levels in the resistant genotype Chiriya-3 (PDI = 1.3) than in the highly susceptible genotype Agra Local (PDI = 40.1). Densitometry analysis showed that expression of an Actin gene was the same at all stages of inoculation due to its constitutive expression in both the resistant and susceptible genotypes, while a 7-fold increase in expression of β-1,3-glucanase II was observed 24 h post inoculation in Chiriya-3. The findings reported in the present study indicate that β-1,3-glucanase production in the B. sorokiniana infected wheat leaves may be involved in resistance to the pathogen.
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Claims 5
Claim · model-written summary
The wheat beta-1,3-glucanase II gene was induced at 24 h post inoculation with Bipolaris sorokiniana.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- inoculatione0
- Interactor
- Bipolaris sorokiniana
Bipolaris sorokinianat1pathogen - Gene
- β-1,3-glucanase IIe0
- Outcome
- β-1,3-glucanase IIe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
t1“Bipolaris sorokiniana”
e0“A gene (β-1,3-glucanase II) was induced at 24 h post inoculation”
Claim · model-written summary
Beta-1,3-glucanase II was induced to higher levels in the resistant genotype Chiriya-3 (PDI 1.3) than in the highly susceptible genotype Agra Local (PDI 40.1).
- Taxon
- wheatTriticum aestivumt0
- Material
- Chiriya-3e0
- Gene
- β-1,3-glucanase IIe0
- Outcome
- β-1,3-glucanase IIe0
- Direction
- highere0
- Qualifier
- PDI = 1.3e0
- Comparator
- highly susceptible genotype Agra Locale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“A gene (β-1,3-glucanase II) was induced at 24 h post inoculation and to higher levels in the resistant genotype Chiriya-3 (PDI = 1.3) than in the highly susceptible genotype Agra Local (PDI = 40.1)”
Claim · model-written summary
Expression of an Actin gene was the same at all stages of inoculation in both resistant and susceptible genotypes (constitutive).
- Taxon
- wheatTriticum aestivumt0
- Treatment
- inoculatione0
- Gene
- Actin genee0
- Outcome
- expression of an Actin genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“expression of an Actin gene was the same at all stages of inoculation”
Claim · model-written summary
Expression of beta-1,3-glucanase II increased 7-fold 24 h post inoculation in Chiriya-3.
- Taxon
- wheatTriticum aestivumt0
- Material
- Chiriya-3e0
- Treatment
- inoculatione0
- Gene
- β-1,3-glucanase IIe0
- Outcome
- expression of β-1,3-glucanase IIe0
- Direction
- increasee0
- Value
- 7-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“a 7-fold increase in expression of β-1,3-glucanase II was observed 24 h post inoculation in Chiriya-3”
Claim · model-written summary
Beta-1,3-glucanase production in B. sorokiniana-infected wheat leaves may be involved in resistance to the pathogen.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- leavese0
- Interactor
- B. sorokinianaBipolaris sorokinianae0pathogen
- Gene
- β-1,3-glucanasee0
- Outcome
- resistance to the pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“β-1,3-glucanase production in the B. sorokiniana infected wheat leaves may be involved in resistance to the pathogen”
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See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.