Paper · 2009 · Journal of Agricultural and Food Chemistry

In Vitro Cultures of Brassica oleracea L. var.costataDC: Potential Plant Bioreactor for Antioxidant Phenolic Compounds

Marcos Taveira; David M. Pereira; Carla Sousa; et al.

5
claims
10
verbatim quotes
37
citations
1
research areas
—
Not open access

Abstract

In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits.

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Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

No phenolic compounds were identified in calli or roots.

Taxon
Brassica oleracea L. var.costataDCBrassica oleraceat0
Outcome
no phenolic compound was identifiede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea
Brassica oleracea · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“In calli and roots no phenolic compound was identified”

✓ located verbatim in the source · abstract, characters 389–443 · show in text ↑
t0

“Brassica oleracea L. var.costataDC”

✓ located verbatim in the source · title, characters 21–55 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Shoots contained 36 compounds.

Taxon
Brassica oleracea L. var.costataDCBrassica oleraceat0
Outcome
presence of 36 compoundse0
Value
36 compoundse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea
Brassica oleracea · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“In shoots was verified the presence of 36 compounds”

✓ located verbatim in the source · abstract, characters 445–496 · show in text ↑
t0

“Brassica oleracea L. var.costataDC”

✓ located verbatim in the source · title, characters 21–55 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The shoot compounds included hydroxycinnamic acids, flavonoids, and hydroxycinnamic acyl glycosides.

Taxon
Brassica oleracea L. var.costataDCBrassica oleraceat0
Outcome
hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosidese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea
Brassica oleracea · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides)”

✓ located verbatim in the source · abstract, characters 498–671 · show in text ↑
t0

“Brassica oleracea L. var.costataDC”

✓ located verbatim in the source · title, characters 21–55 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS liquid medium with BAP and NAA produced the highest shoot phenolic content.

Taxon
Brassica oleracea L. var.costataDCBrassica oleraceat0
Treatment
MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA)e0
Outcome
phenolic compound contentse0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea
Brassica oleracea · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents”

✓ located verbatim in the source · abstract, characters 673–889 · show in text ↑
t0

“Brassica oleracea L. var.costataDC”

✓ located verbatim in the source · title, characters 21–55 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The medium produced stronger antioxidant potential.

Taxon
Brassica oleracea L. var.costataDCBrassica oleraceat0
Treatment
MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA)e0
Outcome
antioxidant potentiale0
Direction
strongere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea
Brassica oleracea · TaxonDaderot · Public domain · Wikimedia Commons ↗

Verbatim

e0

“MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential”

✓ located verbatim in the source · abstract, characters 673–924 · show in text ↑
t0

“Brassica oleracea L. var.costataDC”

✓ located verbatim in the source · title, characters 21–55 · show in text ↑

N-benzyladenine · quality: phytochemicals · Brassica oleracea (wild cabbage)Positive and negative results

5 papers: 4 positive, 1 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

1-naphthaleneacetic acid · quality: phytochemicals · Brassica oleracea (wild cabbage)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

in vitro · quality: phytochemicals · Brassica oleracea (wild cabbage)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.