Abstract
In order to understand the molecular mechanism of the ion-implantation biological effects, we analyzed genes expression profiles in rice seedlings from the seeds implanted with the nitrogen ion beam, using the Agilent Rice Oligo Microarray (4×44) Genome Array. Rice seeds were implanted by the nitrogen ion beam with 40 Kev energy in dose 6×1017 N+/cm2. Total RNA, from the seedlings 96 h after the germination, was used to genechip analysis. The vigor index was measured at 10days after the germination. The results of measuring of the vigor index showed that smaller-dose implantation of the nitrogen ion beam (6×1017 N+/cm2) promoted the vigor index (P<0.05) of the rice seedlings and the larger-dose implantation(9×1017 N+/cm2) damaged the rice seedlings because of the smaller vigor index than the control (P<0.05). The analysis of the genechip array showed that there are 982 transcripts expressed differentially (fold change > 2 and P value<0.05) including 429 up-regulated transcripts and 553 down-regulated transcripts under the dose3 comparing with the controls. 15 out of the 429 up- regulated transcripts were involved in 22 pathways including carbohydrate metabolism, translation, biosynthesis of secondary metabolites, lipid metabolism, energy metabolism, replication and repair, nucleotide metabolism, signal transduction, folding, sorting and degradation and so on. Our analysis supplied the first comprehensive and comparative molecular information for further understanding the mechanism underlying implantation of the low- energy nitrogen ion beam.
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Claims 6
Claim · model-written summary
Nitrogen ion implantation at 6×1017 N+/cm2 increased rice seedling vigor index.
- Taxon
- rice seedlings
Oryza sativae0 - Treatment
- smaller-dose implantation of the nitrogen ion beam (6×1017 N+/cm2)e0
- Outcome
- vigor indexe0
- Direction
- promotede0
- Value
- 6×1017 N+/cm2e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“smaller-dose implantation of the nitrogen ion beam (6×1017 N+/cm2) promoted the vigor index (P<0.05) of the rice seedlings and the larger-dose implantation(9×1017 N+/cm2) damaged the rice seedlings because of the smaller vigor index than the control (P<0.05)”
Claim · model-written summary
Nitrogen ion implantation at 9×1017 N+/cm2 reduced rice seedling vigor index relative to control.
- Taxon
- rice seedlingsOryza sativae0
- Treatment
- larger-dose implantation(9×1017 N+/cm2)e0
- Outcome
- vigor indexe0
- Direction
- smallere0
- Value
- 9×1017 N+/cm2e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“smaller-dose implantation of the nitrogen ion beam (6×1017 N+/cm2) promoted the vigor index (P<0.05) of the rice seedlings and the larger-dose implantation(9×1017 N+/cm2) damaged the rice seedlings because of the smaller vigor index than the control (P<0.05)”
Claim · model-written summary
Under dose3, rice showed 982 transcripts versus controls.
- Taxon
- Rice SeedlingsOryza sativat0
- Treatment
- dose3e0
- Outcome
- transcripts expressed differentiallye0
- Direction
- differentiallye0
- Value
- 982 transcriptse0
- Qualifier
- fold change > 2 and P value<0.05e0
- Comparator
- the controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are 982 transcripts expressed differentially (fold change > 2 and P value<0.05) including 429 up-regulated transcripts and 553 down-regulated transcripts under the dose3 comparing with the controls”
t0“Rice Seedlings”
Claim · model-written summary
Under dose3, rice showed 429 up-regulated transcripts versus controls.
- Taxon
- Rice SeedlingsOryza sativat0
- Treatment
- dose3e0
- Outcome
- up-regulated transcriptse0
- Direction
- up-regulatede0
- Value
- 429 up-regulated transcriptse0
- Qualifier
- fold change > 2 and P value<0.05e0
- Comparator
- the controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are 982 transcripts expressed differentially (fold change > 2 and P value<0.05) including 429 up-regulated transcripts and 553 down-regulated transcripts under the dose3 comparing with the controls”
t0“Rice Seedlings”
Claim · model-written summary
Under dose3, rice showed 553 down-regulated transcripts versus controls.
- Taxon
- Rice SeedlingsOryza sativat0
- Treatment
- dose3e0
- Outcome
- down-regulated transcriptse0
- Direction
- down-regulatede0
- Value
- 553 down-regulated transcriptse0
- Qualifier
- fold change > 2 and P value<0.05e0
- Comparator
- the controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are 982 transcripts expressed differentially (fold change > 2 and P value<0.05) including 429 up-regulated transcripts and 553 down-regulated transcripts under the dose3 comparing with the controls”
t0“Rice Seedlings”
Claim · model-written summary
Fifteen up-regulated transcripts were involved in 22 pathways across metabolic and cellular processes.
- Taxon
- Rice SeedlingsOryza sativat0
- Outcome
- up- regulated transcriptse0
- Value
- 15 out of the 429 up- regulated transcriptse0
- Qualifier
- 22 pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“15 out of the 429 up- regulated transcripts were involved in 22 pathways including carbohydrate metabolism, translation, biosynthesis of secondary metabolites, lipid metabolism, energy metabolism, replication and repair, nucleotide metabolism, signal transduction, folding, sorting and degradation and so on.”
t0“Rice Seedlings”
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See all 511 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.