Abstract
The avirulence gene AVR-Pia of Magnaporthe oryzae, which induces a hypersensitive reaction in rice cultivars containing the resistance gene Pia, was expressed in Escherichia coli. AVR-Pia protein was collected as inclusion bodies, denatured, and refolded. Finally, recombinant AVR-Pia (rAVR-Pia) protein was purified by column chromatography. Infiltration of rAVR-Pia triggered cell browning in the leaves of rice cultivar Aichiasahi (Pia), with accumulation of H2O2 and induction of PR1a expression in rice. On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment. This observation indicated that rAVR-Pia had the function of an avirulence protein. rAVR-Pia was used for immunization of a rabbit, and anti-AVR-Pia antiserum was prepared. The specificity of this antibody was appraised by detecting native AVR-Pia in the inoculated leaf sheath extract using Western blotting in combination with immunoprecipitation. Native AVR-Pia was successfully detected, and its molecular weight was estimated to be 7.4 kDa, indicating signal peptide cleavage. Additionally, secreted native AVR-Pia was quantified as 3.7 ng/g rice sheath.
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Claims 8
Claim · model-written summary
rAVR-Pia infiltration triggered cell browning in Aichiasahi rice leaves.
- Taxon
- rice
Oryza sativae0 - Material
- Aichiasahi (Pia)e0
- Treatment
- Infiltration of rAVR-Piae0
- Interactor
- Magnaporthe oryzae
Pyricularia oryzaet0pathogen - Gene
- rAVR-Piae0
- Outcome
- cell browninge0
- Comparator
- Shin-2 (pia)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infiltration of rAVR-Pia triggered cell browning in the leaves of rice cultivar Aichiasahi (Pia), with accumulation of H2O2 and induction of PR1a expression in rice. On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment.”
t0“Magnaporthe oryzae”
Claim · model-written summary
rAVR-Pia infiltration caused H2O2 accumulation in Aichiasahi rice leaves.
- Taxon
- riceOryza sativae0
- Material
- Aichiasahi (Pia)e0
- Treatment
- Infiltration of rAVR-Piae0
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Outcome
- accumulation of H2O2e0
- Direction
- accumulatione0
- Comparator
- Shin-2 (pia)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infiltration of rAVR-Pia triggered cell browning in the leaves of rice cultivar Aichiasahi (Pia), with accumulation of H2O2 and induction of PR1a expression in rice. On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment.”
t0“Magnaporthe oryzae”
Claim · model-written summary
rAVR-Pia infiltration induced PR1a expression in Aichiasahi rice leaves.
- Taxon
- riceOryza sativae0
- Material
- Aichiasahi (Pia)e0
- Treatment
- Infiltration of rAVR-Piae0
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Gene
- PR1ae0
- Outcome
- PR1a expressione0
- Direction
- inductione0
- Comparator
- Shin-2 (pia)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infiltration of rAVR-Pia triggered cell browning in the leaves of rice cultivar Aichiasahi (Pia), with accumulation of H2O2 and induction of PR1a expression in rice. On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment.”
t0“Magnaporthe oryzae”
Claim · model-written summary
Shin-2 rice leaves did not show browning, H2O2 accumulation, or PR1a induction after rAVR-Pia infiltration.
- Taxon
- riceOryza sativae1
- Material
- Shin-2 (pia)e0
- Treatment
- same treatmente0
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Outcome
- these reactionse0
- Comparator
- Aichiasahi (Pia)e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment.”
t0“Magnaporthe oryzae avirulence protein AVR-Pia”
e1“Infiltration of rAVR-Pia triggered cell browning in the leaves of rice cultivar Aichiasahi (Pia), with accumulation of H2O2 and induction of PR1a expression in rice. On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment.”
Claim · model-written summary
An antibody method detected native AVR-Pia in inoculated rice leaf sheath.
- Taxon
- riceOryza sativae1
- Organ / stage
- leaf sheathe0
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Gene
- AVR-Piae0
- Outcome
- Native AVR-Pia was successfully detectede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The specificity of this antibody was appraised by detecting native AVR-Pia in the inoculated leaf sheath extract using Western blotting in combination with immunoprecipitation. Native AVR-Pia was successfully detected, and its molecular weight was estimated to be 7.4 kDa, indicating signal peptide cleavage.”
t0“Magnaporthe oryzae avirulence protein AVR-Pia”
e1“Infiltration of rAVR-Pia triggered cell browning in the leaves of rice cultivar Aichiasahi (Pia), with accumulation of H2O2 and induction of PR1a expression in rice. On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment.”
Claim · model-written summary
Detected native AVR-Pia had an estimated molecular weight of 7.4 kDa.
- Taxon
- riceOryza sativae1
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Gene
- AVR-Piae0
- Outcome
- molecular weighte0
- Value
- 7.4 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Native AVR-Pia was successfully detected, and its molecular weight was estimated to be 7.4 kDa, indicating signal peptide cleavage.”
t0“Magnaporthe oryzae avirulence protein AVR-Pia”
e1“Infiltration of rAVR-Pia triggered cell browning in the leaves of rice cultivar Aichiasahi (Pia), with accumulation of H2O2 and induction of PR1a expression in rice. On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment.”
Claim · model-written summary
The authors interpret the 7.4 kDa native AVR-Pia size as indicating signal peptide cleavage.
- Taxon
- riceOryza sativae1
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Gene
- AVR-Piat0
- Outcome
- signal peptide cleavagee0
- Qualifier
- molecular weight was estimated to be 7.4 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“molecular weight was estimated to be 7.4 kDa, indicating signal peptide cleavage.”
t0“Magnaporthe oryzae avirulence protein AVR-Pia”
e1“Infiltration of rAVR-Pia triggered cell browning in the leaves of rice cultivar Aichiasahi (Pia), with accumulation of H2O2 and induction of PR1a expression in rice. On the other hand, these reactions were not observed in Shin-2 (pia) leaves after the same treatment.”
Claim · model-written summary
Secreted native AVR-Pia was quantified at 3.7 ng/g rice sheath.
- Taxon
- riceOryza sativae0
- Organ / stage
- sheathe0
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Gene
- AVR-Piae0
- Outcome
- secreted native AVR-Piae0
- Value
- 3.7 ng/g rice sheathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Additionally, secreted native AVR-Pia was quantified as 3.7 ng/g rice sheath.”
t0“Magnaporthe oryzae”