Abstract
Nitric oxide (NO) generation by soybean (Glycine max, var ADM 4800) chloroplasts was studied by electron paramagnetic resonance (EPR) spin-trapping technique.1 Both nitrite and L-arginine (arg) are the required substrates for enzymatic activities considered as possible sources of NO in plants. Soybean chloroplasts showed a NO production of 3.2 +/- 0.2 nmol min(-1) mg(-1) protein in the presence of 1 mM NaNO(2). Chloroplasts incubated with 1 mM arg showed a NO production of 0.76 +/- 0.04 nmol min(-1) mg(-1) protein. This production was inhibited when chloroplasts were incubated in presence of NOS-inhibitors L-NAME and L-NNA. In vitro exposure of chloroplasts to a NO-donor (GSNO) decreased both ascorbyl radical content and the activity of ascorbate peroxidase, without modification of the total ascorbate content. Exposure of the isolated chloroplasts to a NO-donor decreased lipid radical content in membranes, however, incubation in the presence of 25 microM peroxynitrite (ONOO(-)) led to an increase in lipid-derived radicals (34%). The effect of ONOO(-) on protein oxidation was determined by western blotting, showing an increase in carbonyl content either in stroma or thylakoid proteins as compared to control. Taken as a whole, NO seems to be an endogenous metabolite in soybean chloroplasts and reactive nitrogen species could exert either antioxidant or prooxidant effects on chloroplasts, since both a decreased lipid radical content in membranes and a decrease in the activity of ascorbate peroxidase were observed after exposure to a NO donor.
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Claims 10
Claim · model-written summary
Soybean chloroplasts produced NO at 3.2 +/- 0.2 nmol min(-1) mg(-1) protein with 1 mM NaNO(2).
- Taxon
- Soybean
Glycine maxt0 - Organ / stage
- chloroplastse0
- Treatment
- 1 mM NaNO(2)e0
- Outcome
- NO productione0
- Value
- 3.2 +/- 0.2 nmol min(-1) mg(-1) proteine0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean chloroplasts showed a NO production of 3.2 +/- 0.2 nmol min(-1) mg(-1) protein in the presence of 1 mM NaNO(2).”
t0“Soybean”
Claim · model-written summary
Soybean chloroplasts produced NO at 0.76 +/- 0.04 nmol min(-1) mg(-1) protein with 1 mM arg.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 1 mM arge0
- Outcome
- NO productione0
- Value
- 0.76 +/- 0.04 nmol min(-1) mg(-1) proteine0
Not reportedOrgan / stageComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chloroplasts incubated with 1 mM arg showed a NO production of 0.76 +/- 0.04 nmol min(-1) mg(-1) protein.”
t0“Soybean”
Claim · model-written summary
NOS inhibitors L-NAME and L-NNA inhibited NO production.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- chloroplastse0
- Treatment
- NOS-inhibitors L-NAME and L-NNAe0
- Outcome
- productione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This production was inhibited when chloroplasts were incubated in presence of NOS-inhibitors L-NAME and L-NNA.”
t0“Soybean”
Claim · model-written summary
GSNO decreased ascorbyl radical content.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- chloroplastse0
- Treatment
- NO-donor (GSNO)e0
- Outcome
- ascorbyl radical contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro exposure of chloroplasts to a NO-donor (GSNO) decreased both ascorbyl radical content and the activity of ascorbate peroxidase, without modification of the total ascorbate content.”
t0“Soybean”
Claim · model-written summary
GSNO decreased ascorbate peroxidase activity.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- chloroplastse0
- Treatment
- NO-donor (GSNO)e0
- Outcome
- activity of ascorbate peroxidasee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro exposure of chloroplasts to a NO-donor (GSNO) decreased both ascorbyl radical content and the activity of ascorbate peroxidase, without modification of the total ascorbate content.”
t0“Soybean”
Claim · model-written summary
GSNO did not change total ascorbate content.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- chloroplastse0
- Treatment
- NO-donor (GSNO)e0
- Outcome
- total ascorbate contente0
- Direction
- without modificatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro exposure of chloroplasts to a NO-donor (GSNO) decreased both ascorbyl radical content and the activity of ascorbate peroxidase, without modification of the total ascorbate content.”
t0“Soybean”
Claim · model-written summary
A NO donor decreased lipid radical content in chloroplast membranes.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- chloroplastse0
- Treatment
- NO-donore0
- Outcome
- lipid radical contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of the isolated chloroplasts to a NO-donor decreased lipid radical content in membranes”
t0“Soybean”
Claim · model-written summary
Peroxynitrite increased lipid-derived radicals by 34%.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 25 microM peroxynitrite (ONOO(-))e0
- Outcome
- lipid-derived radicalse0
- Direction
- increasee0
- Value
- 34%e0
Not reportedOrgan / stageComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“incubation in the presence of 25 microM peroxynitrite (ONOO(-)) led to an increase in lipid-derived radicals (34%).”
t0“Soybean”
Claim · model-written summary
Peroxynitrite increased carbonyl content in stroma and thylakoid proteins relative to control.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- ONOO(-)e0
- Outcome
- carbonyl contente0
- Direction
- increasee0
Not reportedOrgan / stageComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of ONOO(-) on protein oxidation was determined by western blotting, showing an increase in carbonyl content either in stroma or thylakoid proteins as compared to control.”
t0“Soybean”
Claim · model-written summary
NO appears to be an endogenous metabolite in soybean chloroplasts.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- chloroplastse0
- Outcome
- endogenous metabolitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO seems to be an endogenous metabolite in soybean chloroplasts”
The same questions in other papers
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1 in the opposite direction
- positive
nitric oxide donor · molecular: metabolites · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →peroxynitrite · molecular: metabolites · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.