Abstract
Experiments were carried out to evaluate the effects of exposure to nitric oxide on the ability by NADPH‐dependent microsomal electron transfer to generate oxygen radicals. Such interactions could play a role in the potential antioxidant action of nitric oxide (NO). Isolated microsomes from soybean ( Glycine max [L.] Merr. cv. Hood) embryonic axes were exposed to an exogenously added source of nitric oxide (NO) (S‐nitrosoglutathione + dithiothreitol). The O 2 − generation rate by microsomes exposed to NO decreased significantly as compared to the rate measured in microsomes incubated in the absence of NO. The exposure of the microsomes to the NO donor did not alter the microsomal rate of hydroxyl radical generation. Preincubation of the microsomes with the NO donor affected neither iron reduction rate nor activity of cytochrome c reductase. However, cytochrome P 450 activity was significantly inhibited after exposure to NO. This inhibition was completely prevented by hemoglobin. The data are consistent with the hypothesis that NO exhibits a potential antioxidant role in the plant cell by decreasing the rate of generation of superoxide anion. Since endogenous NO was detected in homogenates of soybean embryonic axes by EPR studies, this interaction between NO and cytochrome P 450 in soybean embryonic axes could be a factor of relevance for the control of oxidative stress in vivo.
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Claims 6
Claim · model-written summary
Exposure of soybean embryonic-axis microsomes to nitric oxide significantly decreased the O2 superoxide generation rate compared with unexposed microsomes.
- Taxon
- soybean
Glycine maxt0 - Material
- cv. Hoode1
- Organ / stage
- embryonic axese1
- Treatment
- exogenously added source of nitric oxidee1
- Outcome
- O 2 − generation ratee2
- Direction
- decreasede2
- Comparator
- microsomes incubated in the absence of NOe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Isolated microsomes from soybean ( Glycine max [L.] Merr. cv. Hood) embryonic axes were exposed to an exogenously added source of nitric oxide (NO)”
e2“The O 2 − generation rate by microsomes exposed to NO decreased significantly as compared to the rate measured in microsomes incubated in the absence of NO”
t0“microsomes from soybean embryonic axes”
Claim · model-written summary
The NO donor did not alter the hydroxyl radical generation rate of soybean microsomes.
- Taxon
- soybeanGlycine maxt0
- Treatment
- NO donore3
- Outcome
- rate of hydroxyl radical generatione3
- Direction
- did not altere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The exposure of the microsomes to the NO donor did not alter the microsomal rate of hydroxyl radical generation”
t0“microsomes from soybean embryonic axes”
Claim · model-written summary
NO donor preincubation affected neither the iron reduction rate nor cytochrome c reductase activity of soybean microsomes.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Preincubation of the microsomes with the NO donore4
- Outcome
- iron reduction rate nor activity of cytochrome c reductasee4
- Direction
- affected neithere4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Preincubation of the microsomes with the NO donor affected neither iron reduction rate nor activity of cytochrome c reductase”
t0“microsomes from soybean embryonic axes”
Claim · model-written summary
NO exposure significantly inhibited cytochrome P450 activity in soybean microsomes.
- Taxon
- soybeanGlycine maxt0
- Treatment
- exposure to NOe5
- Outcome
- cytochrome P 450 activitye5
- Direction
- inhibitede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“cytochrome P 450 activity was significantly inhibited after exposure to NO”
t0“microsomes from soybean embryonic axes”
Claim · model-written summary
Hemoglobin completely prevented the inhibition of cytochrome P450 activity caused by NO.
- Taxon
- soybeanGlycine maxt0
- Treatment
- hemoglobine6
- Outcome
- inhibitione6
- Direction
- completely preventede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“This inhibition was completely prevented by hemoglobin”
t0“microsomes from soybean embryonic axes”
Claim · model-written summary
Endogenous NO was detected in homogenates of soybean embryonic axes by EPR.
- Taxon
- soybeanGlycine maxe7
- Organ / stage
- embryonic axese7
- Outcome
- endogenous NOe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Since endogenous NO was detected in homogenates of soybean embryonic axes by EPR studies”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.