Abstract
The hypothesis that arachidonic acid (AA) induction of sesquiterpene accumulation and browning in potato (Solanum tuberosum) is mediated by a lipoxygenase metabolite of AA was tested using lipoxygenase inhibitors. Salicylhydroxamic acid (SHAM) and 3-amino-1-(3-trifluoromethylphenyl)-2-pyrazoline hydrochloride (BW755C) delayed the response to AA. Inhibition by eicosatetraynoic acid (ETYA) was more persistent. These results are consistent with previous reports that SHAM and BW755C are reversible inhibitors of lipoxygenase and easily oxidized by potato while ETYA acts as an irreversible inhibitor. Disulfiram (tetraethylthiuram disulfide) also inhibited AA elicitor activity. SHAM was most effective if applied at the time of AA treatment, having no effect if applied 6 hours afterward. SHAM was effective in the presence of MES or MOPS buffers but not in acetate-buffered or unbuffered solutions; neither BW755C nor ETYA exhibited this restriction. However, SHAM, BW755C, and ETYA also were inhibitors of browning and sesquiterpene accumulation elicited in potato by poly-l-lysine, which, unlike AA, is not a lipoxygenase substrate. SHAM effectiveness also was restricted to 6 hours after treatment with poly-l-lysine. While the results with AA support a role for lipoxygenase, those with poly-l-lysine may be evidence that these compounds are having other effects in potato tissue.
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Claims 16
Claim · model-written summary
SHAM delayed the potato response to arachidonic acid.
- Taxon
- potato
Solanum tuberosumt0 - Treatment
- Salicylhydroxamic acide0
- Outcome
- response to AAe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salicylhydroxamic acid (SHAM) and 3-amino-1-(3-trifluoromethylphenyl)-2-pyrazoline hydrochloride (BW755C) delayed the response to AA.”
t0“Potato”
Claim · model-written summary
BW755C delayed the potato response to arachidonic acid.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- 3-amino-1-(3-trifluoromethylphenyl)-2-pyrazoline hydrochloridee0
- Outcome
- response to AAe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salicylhydroxamic acid (SHAM) and 3-amino-1-(3-trifluoromethylphenyl)-2-pyrazoline hydrochloride (BW755C) delayed the response to AA.”
t0“Potato”
Claim · model-written summary
ETYA inhibited the potato response more persistently.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- eicosatetraynoic acide0
- Outcome
- Inhibitione0
- Direction
- more persistente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition by eicosatetraynoic acid (ETYA) was more persistent.”
t0“Potato”
Claim · model-written summary
Disulfiram inhibited arachidonic acid elicitor activity.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- Disulfirame0
- Outcome
- AA elicitor activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disulfiram (tetraethylthiuram disulfide) also inhibited AA elicitor activity.”
t0“Potato”
Claim · model-written summary
SHAM was most effective when applied with arachidonic acid.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- SHAMe0
- Outcome
- most effectivee0
- Direction
- most effectivee0
- Comparator
- at the time of AA treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM was most effective if applied at the time of AA treatment”
t0“Potato”
Claim · model-written summary
SHAM had no effect if applied six hours after arachidonic acid.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- SHAMe1
- Outcome
- no effecte0
- Direction
- no effecte0
- Qualifier
- 6 hours afterwarde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“having no effect if applied 6 hours afterward.”
t0“Potato”
e1“SHAM was most effective if applied at the time of AA treatment, having no effect if applied 6 hours afterward.”
Claim · model-written summary
SHAM was effective with MES buffer.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- SHAMe0
- Outcome
- effectivee0
- Qualifier
- MESe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM was effective in the presence of MES or MOPS buffers but not in acetate-buffered or unbuffered solutions”
t0“Potato”
Claim · model-written summary
SHAM was effective with MOPS buffer.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- SHAMe0
- Outcome
- effectivee0
- Qualifier
- MOPSe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM was effective in the presence of MES or MOPS buffers but not in acetate-buffered or unbuffered solutions”
t0“Potato”
Claim · model-written summary
SHAM was ineffective in acetate-buffered solutions.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- SHAMe0
- Outcome
- but not in acetate-bufferede0
- Direction
- but note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM was effective in the presence of MES or MOPS buffers but not in acetate-buffered or unbuffered solutions”
t0“Potato”
Claim · model-written summary
SHAM was ineffective in unbuffered solutions.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- SHAMe0
- Outcome
- unbuffered solutionse0
- Direction
- but note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM was effective in the presence of MES or MOPS buffers but not in acetate-buffered or unbuffered solutions”
t0“Potato”
Claim · model-written summary
SHAM inhibited poly-l-lysine-elicited browning and sesquiterpene accumulation.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- SHAMe0
- Outcome
- browning and sesquiterpene accumulatione0
- Direction
- inhibitorse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM, BW755C, and ETYA also were inhibitors of browning and sesquiterpene accumulation elicited in potato by poly-l-lysine”
Claim · model-written summary
BW755C inhibited poly-l-lysine-elicited browning and sesquiterpene accumulation.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- BW755Ce0
- Outcome
- browning and sesquiterpene accumulatione0
- Direction
- inhibitorse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM, BW755C, and ETYA also were inhibitors of browning and sesquiterpene accumulation elicited in potato by poly-l-lysine”
Claim · model-written summary
ETYA inhibited poly-l-lysine-elicited browning and sesquiterpene accumulation.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- ETYAe0
- Outcome
- browning and sesquiterpene accumulatione0
- Direction
- inhibitorse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM, BW755C, and ETYA also were inhibitors of browning and sesquiterpene accumulation elicited in potato by poly-l-lysine”
Claim · model-written summary
SHAM effectiveness against poly-l-lysine response was restricted to the first six hours.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- SHAM effectivenesse0
- Qualifier
- 6 hours after treatment with poly-l-lysinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SHAM effectiveness also was restricted to 6 hours after treatment with poly-l-lysine.”
t0“Potato”
Claim · model-written summary
The authors interpreted AA results as supporting a lipoxygenase role.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- lipoxygenasee0
- Outcome
- role for lipoxygenasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the results with AA support a role for lipoxygenase”
t0“Potato”
Claim · model-written summary
Poly-l-lysine results suggested the inhibitors may have other effects in potato tissue.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- other effects in potato tissuee0
- Qualifier
- these compoundse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those with poly-l-lysine may be evidence that these compounds are having other effects in potato tissue.”
The same questions in other papers
salicylhydroxamic acid · molecular: metabolites · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.