Abstract
B12D belongs to a function unknown subgroup of the Balem (Barley aleurone and embryo) proteins. In our previous work on rice seed germination, we identified a B12D-like protein encoded by LOC_Os7g41350 (named OsB12D1). OsB12D1 pertains to an ancient protein family with an amino acid sequence highly conserved from moss to angiosperms. Among the six OsB12Ds, OsB12D1 is one of the major transcripts and is primarily expressed in germinating seed and root. Bioinformatics analyses indicated that OsB12D1 is an anoxic or submergence resistance-related gene. RT-PCR results showed OsB12D1 is induced remarkably in the coleoptiles or roots by flooding during seed germination and early seedling growth. The OsB12D1-overexpressed rice seeds could protrude radicles in 8 cm deep water, further exhibiting significant flooding tolerance compared to the wild type. Moreover, this tolerance was not affected by the gibberellin biosynthesis inhibitor paclobutrazol. OsB12D1 was identified in the mitochondrion by subcellular localization analysis and possibly enhances electron transport through mediating Fe and oxygen availability under flooded conditions. This work indicated that OsB12D1 is a promising gene that can help to enhance rice seedling establishment in farming practices, especially for direct seeding.
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Claims 9
Claim · model-written summary
OsB12D1 was primarily expressed in germinating rice seed and root.
- Taxon
- Rice
Oryza sativat0 - Organ / stage
- germinating seed and roote1
- Gene
- OsB12D1e1
- Outcome
- primarily expressede1
- Qualifier
- one of the major transcriptse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Among the six OsB12Ds, OsB12D1 is one of the major transcripts and is primarily expressed in germinating seed and root”
t0“in Rice”
Claim · model-written summary
OsB12D1 was predicted by bioinformatics to be an anoxic or submergence resistance-related gene.
- Taxon
- RiceOryza sativat0
- Gene
- OsB12D1e2
- Outcome
- anoxic or submergence resistance-related genee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Bioinformatics analyses indicated that OsB12D1 is an anoxic or submergence resistance-related gene”
t0“in Rice”
Claim · model-written summary
OsB12D1 was induced remarkably in rice coleoptiles or roots by flooding during germination and early seedling growth.
- Taxon
- RiceOryza sativat0
- Organ / stage
- coleoptiles or rootse3
- Treatment
- floodinge3
- Gene
- OsB12D1e3
- Outcome
- OsB12D1 is inducede3
- Direction
- induced remarkablye3
- Qualifier
- during seed germination and early seedling growthe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“RT-PCR results showed OsB12D1 is induced remarkably in the coleoptiles or roots by flooding during seed germination and early seedling growth”
t0“in Rice”
Claim · model-written summary
OsB12D1-overexpressed rice seeds showed significant flooding tolerance compared to wild type.
- Taxon
- riceOryza sativae4
- Material
- OsB12D1-overexpressede4
- Treatment
- 8 cm deep watere4
- Outcome
- flooding tolerancee4
- Comparator
- wild typee4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The OsB12D1-overexpressed rice seeds could protrude radicles in 8 cm deep water, further exhibiting significant flooding tolerance compared to the wild type”
Claim · model-written summary
OsB12D1-overexpressed rice seeds protruded radicles in 8 cm deep water.
- Taxon
- riceOryza sativae4
- Material
- OsB12D1-overexpressede4
- Organ / stage
- seedse4
- Treatment
- 8 cm deep watere4
- Outcome
- protrude radiclese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The OsB12D1-overexpressed rice seeds could protrude radicles in 8 cm deep water, further exhibiting significant flooding tolerance compared to the wild type”
Claim · model-written summary
Paclobutrazol did not affect the flooding tolerance conferred by OsB12D1 in rice.
- Taxon
- RiceOryza sativat0
- Treatment
- paclobutrazole5
- Outcome
- tolerancee5
- Direction
- not affectede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Moreover, this tolerance was not affected by the gibberellin biosynthesis inhibitor paclobutrazol”
t0“in Rice”
Claim · model-written summary
OsB12D1 was identified in the mitochondrion of rice by subcellular localization analysis.
- Taxon
- RiceOryza sativat0
- Gene
- OsB12D1e6
- Outcome
- identified in the mitochondrione6
- Qualifier
- by subcellular localization analysise6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“OsB12D1 was identified in the mitochondrion by subcellular localization analysis and possibly enhances electron transport through mediating Fe and oxygen availability under flooded conditions”
t0“in Rice”
Claim · model-written summary
OsB12D1 possibly enhances electron transport in rice by mediating Fe and oxygen availability under flooding.
- Taxon
- RiceOryza sativat0
- Gene
- OsB12D1e6
- Outcome
- electron transporte6
- Qualifier
- through mediating Fe and oxygen availability under flooded conditionse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“OsB12D1 was identified in the mitochondrion by subcellular localization analysis and possibly enhances electron transport through mediating Fe and oxygen availability under flooded conditions”
t0“in Rice”
Claim · model-written summary
OsB12D1 is a promising gene for enhancing rice seedling establishment, especially for direct seeding.
- Taxon
- riceOryza sativae7
- Gene
- OsB12D1e7
- Outcome
- rice seedling establishmente7
- Qualifier
- especially for direct seedinge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“OsB12D1 is a promising gene that can help to enhance rice seedling establishment in farming practices, especially for direct seeding”
The same questions in other papers
paclobutrazol · disease · Oryza sativa (rice)Results differ
1 paper in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.