Abstract
The disappearance of nitrate reductase activity in leaves of Hordeum vulgare L. during darkness was inhibited by cycloheximide, actinomycin D, and low temperature. Thus, protein synthesis was probably required for the disappearance of nitrate reductase in the dark. Since chloramphenicol did not affect the rate of loss of activity, the degradation or inactivation apparently required protein synthesis by the cytoplasmic ribosomal system. Consistent with this observation, nitrate reductase is also reportedly located in the cytoplasm. Thus, the amount of nitrate reductase activity present in leaves of barley may be controlled by a balance between activating and inactivating systems.
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Claims 7
Claim · model-written summary
Cycloheximide inhibited disappearance of nitrate reductase activity in barley leaves during darkness.
- Taxon
- Hordeum vulgare L.
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- leavese0
- Treatment
- cycloheximidee0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The disappearance of nitrate reductase activity in leaves of Hordeum vulgare L. during darkness was inhibited by cycloheximide, actinomycin D, and low temperature”
Claim · model-written summary
Actinomycin D inhibited disappearance of nitrate reductase activity in barley leaves during darkness.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree0
- Organ / stage
- leavese0
- Treatment
- actinomycin De0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disappearance of nitrate reductase activity in leaves of Hordeum vulgare L. during darkness was inhibited by cycloheximide, actinomycin D, and low temperature”
Claim · model-written summary
Low temperature inhibited disappearance of nitrate reductase activity in barley leaves during darkness.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree0
- Organ / stage
- leavese0
- Treatment
- low temperaturee0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disappearance of nitrate reductase activity in leaves of Hordeum vulgare L. during darkness was inhibited by cycloheximide, actinomycin D, and low temperature”
Claim · model-written summary
Chloramphenicol did not affect the rate of nitrate reductase activity loss.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Treatment
- chloramphenicole0
- Gene
- nitrate reductasee1
- Outcome
- rate of loss of activitye0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Since chloramphenicol did not affect the rate of loss of activity”
e1“nitrate reductase activity in leaves of Hordeum vulgare L.”
Claim · model-written summary
Protein synthesis was probably required for dark disappearance of nitrate reductase activity.
- Taxon
- Hordeum vulgare L.Hordeum vulgaree1
- Gene
- nitrate reductasee0
- Outcome
- disappearance of nitrate reductasee0
- Qualifier
- protein synthesis was probably requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protein synthesis was probably required for the disappearance of nitrate reductase in the dark”
e1“Hordeum vulgare L.”
Claim · model-written summary
The degradation or inactivation of nitrate reductase apparently required cytoplasmic ribosomal protein synthesis.
- Taxon
- Hordeum vulgare L.Hordeum vulgaret0
- Gene
- nitrate reductasee1
- Outcome
- degradation or inactivatione0
- Qualifier
- protein synthesis by the cytoplasmic ribosomal systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the degradation or inactivation apparently required protein synthesis by the cytoplasmic ribosomal system”
e1“the disappearance of nitrate reductase in the dark”
t0“Hordeum vulgare L.”
Claim · model-written summary
Barley leaf nitrate reductase activity may be controlled by a balance of activating and inactivating systems.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- leavese0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Qualifier
- balance between activating and inactivating systemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amount of nitrate reductase activity present in leaves of barley may be controlled by a balance between activating and inactivating systems”
The same questions in other papers
actinomycin D · physiology: enzyme activity · Hordeum vulgare (barley)Positive and negative results
2 in the opposite direction
- negative
- no effect
cycloheximide · physiology: enzyme activity · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- no effect
3 papers more in this group
See all 7 papers →cold · physiology: enzyme activity · Hordeum vulgare (barley)Positive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.