Abstract
Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers. Previous work has demonstrated de novo synthesis of alpha-amylase by gibberellic acid in the same tissue. The increase in nitrate reductase activity is inhibited by cycloheximide and 6-methylpurine, but not by actinomycin D. Nitrate does not induce alpha-amylase synthesis, and it has no effect on the gibberellic acid-induced synthesis of alpha-amylase. Also, there is little or no direct effect of gibberellic acid (during the first 6 hr of induction) or of abscisic acid on the nitrate-induced formation of nitrate reductase. Gibberellic acid does interfere with nitrate reductase activity during long-term experiments (greater than 6 hr). However, the time course of this inhibition suggests that the inhibition may be a secondary one. Barley aleurone layers therefore provide a convenient tissue for the study of both substrate- and hormone-induced enzyme formation.
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Claims 10
Claim · model-written summary
Nitrate induced the formation of nitrate reductase activity in barley aleurone layers.
- Taxon
- barley
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Hordeum vulgaree1 - Material
- cv. Himalayae1
- Organ / stage
- aleurone layerse1
- Treatment
- Nitratee1
- Outcome
- formation of nitrate reductase activitye1
- Direction
- inducese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
Claim · model-written summary
Nitrate did not induce alpha-amylase synthesis in barley aleurone layers.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Nitratee2
- Outcome
- alpha-amylase synthesise2
- Direction
- does not inducee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
e2“Nitrate does not induce alpha-amylase synthesis, and it has no effect on the gibberellic acid-induced synthesis of alpha-amylase”
Claim · model-written summary
Nitrate had no effect on gibberellic acid-induced alpha-amylase synthesis.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Nitratee2
- Compound
- gibberellic acide2
- Outcome
- gibberellic acid-induced synthesis of alpha-amylasee2
- Direction
- no effecte2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
e2“Nitrate does not induce alpha-amylase synthesis, and it has no effect on the gibberellic acid-induced synthesis of alpha-amylase”
Claim · model-written summary
Cycloheximide inhibited the nitrate-induced increase in nitrate reductase activity.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- cycloheximidee3
- Outcome
- increase in nitrate reductase activitye3
- Direction
- inhibitede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
e3“The increase in nitrate reductase activity is inhibited by cycloheximide and 6-methylpurine, but not by actinomycin D”
Claim · model-written summary
6-methylpurine inhibited the nitrate-induced increase in nitrate reductase activity.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- 6-methylpurinee3
- Outcome
- increase in nitrate reductase activitye3
- Direction
- inhibitede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
e3“The increase in nitrate reductase activity is inhibited by cycloheximide and 6-methylpurine, but not by actinomycin D”
Claim · model-written summary
Actinomycin D did not inhibit the nitrate-induced increase in nitrate reductase activity.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- actinomycin De3
- Outcome
- increase in nitrate reductase activitye3
- Direction
- but not bye3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
e3“The increase in nitrate reductase activity is inhibited by cycloheximide and 6-methylpurine, but not by actinomycin D”
Claim · model-written summary
Gibberellic acid had little or no direct effect on nitrate-induced nitrate reductase formation during the first 6 hr.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- gibberellic acide4
- Outcome
- nitrate-induced formation of nitrate reductasee4
- Direction
- little or no direct effecte4
- Qualifier
- during the first 6 hr of inductione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
e4“there is little or no direct effect of gibberellic acid (during the first 6 hr of induction) or of abscisic acid on the nitrate-induced formation of nitrate reductase”
Claim · model-written summary
Abscisic acid had little or no direct effect on nitrate-induced nitrate reductase formation.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- abscisic acide4
- Outcome
- nitrate-induced formation of nitrate reductasee4
- Direction
- little or no direct effecte4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
e4“there is little or no direct effect of gibberellic acid (during the first 6 hr of induction) or of abscisic acid on the nitrate-induced formation of nitrate reductase”
Claim · model-written summary
Gibberellic acid interfered with nitrate reductase activity in long-term experiments (greater than 6 hr).
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Gibberellic acide5
- Outcome
- nitrate reductase activitye5
- Direction
- interfere withe5
- Qualifier
- during long-term experiments (greater than 6 hr)e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers”
e5“Gibberellic acid does interfere with nitrate reductase activity during long-term experiments (greater than 6 hr)”
Claim · model-written summary
Barley aleurone layers are a convenient tissue for studying substrate- and hormone-induced enzyme formation.
- Taxon
- BarleyHordeum vulgaree6
- Outcome
- convenient tissue for the study of both substrate- and hormone-induced enzyme formatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Barley aleurone layers therefore provide a convenient tissue for the study of both substrate- and hormone-induced enzyme formation”
The same questions in other papers
actinomycin D · physiology: enzyme activity · Hordeum vulgare (barley)Positive and negative results
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See all 17 papers →6-methylpurine · physiology: enzyme activity · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- negative
nitrogen · physiology: enzyme activity · Hordeum vulgare (barley)Positive and negative results
10 papers in the same direction as this paper · 2 in the opposite direction
- positive
- no effect
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.