Abstract
When mitochondria isolated from etiolated pea (Pisum sativum cv. Alaska) epicotyls were exposed briefly to red light, their ability to reduce exogenous NADP was enhanced. The red light effect was reversed by far red light. Photoreversible absorbance changes between 730 nm and 800 nm were spectrophotometrically detected in the purified mitochondria and its membrane fraction. The dehydrogenase activity in the mitochondria was heat-labile and was dependent on the presence of magnesium ion and appropriate substrates such as glucose 6-phosphate, isocitrate, pyruvate, 6-phosphogluconate, and succinate. The photoreversible effect was seen only for a few minutes after the irradiation, and was cancelled by hypotonic treatment or addition of Triton X-100. A similar but lesser effect was observed in the pea microsome fraction, whereas no photoreversible response was seen with a supernatant fraction resulting from centrifugation at 10(5)g for 30 minutes.
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Claims 10
Claim · model-written summary
Red light enhanced NADP reduction by mitochondria from etiolated pea epicotyls.
- Taxon
- pea
Lathyrus oleraceuse0 - Material
- Alaskae0
- Organ / stage
- etiolated pea (Pisum sativum cv. Alaska) epicotylse0
- Treatment
- red lighte0
- Outcome
- ability to reduce exogenous NADPe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When mitochondria isolated from etiolated pea (Pisum sativum cv. Alaska) epicotyls were exposed briefly to red light, their ability to reduce exogenous NADP was enhanced”
Claim · model-written summary
Far red light reversed the red-light effect on pea mitochondrial NADP reduction.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- far red lighte0
- Outcome
- ability to reduce exogenous NADPe2
- Direction
- reversede0
- Comparator
- red lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The red light effect was reversed by far red light”
e1“pea (Pisum sativum cv. Alaska) epicotyls”
e2“ability to reduce exogenous NADP”
Claim · model-written summary
Purified pea mitochondria and their membrane fraction showed photoreversible absorbance changes between 730 and 800 nm.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- Photoreversible absorbance changese0
- Value
- between 730 nm and 800 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Photoreversible absorbance changes between 730 nm and 800 nm were spectrophotometrically detected in the purified mitochondria and its membrane fraction”
e1“pea (Pisum sativum cv. Alaska) epicotyls”
Claim · model-written summary
Pea mitochondrial dehydrogenase activity was heat-labile.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- dehydrogenase activity in the mitochondria was heat-labilee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The dehydrogenase activity in the mitochondria was heat-labile”
e1“pea (Pisum sativum cv. Alaska) epicotyls”
Claim · model-written summary
Pea mitochondrial dehydrogenase activity depended on magnesium ion and suitable substrates.
- Taxon
- peaLathyrus oleraceuse2
- Compound
- magnesium ione0
- Outcome
- dehydrogenase activitye1
- Direction
- dependente0
- Qualifier
- appropriate substrates such as glucose 6-phosphate, isocitrate, pyruvate, 6-phosphogluconate, and succinatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was dependent on the presence of magnesium ion and appropriate substrates such as glucose 6-phosphate, isocitrate, pyruvate, 6-phosphogluconate, and succinate”
e1“The dehydrogenase activity in the mitochondria”
e2“pea (Pisum sativum cv. Alaska) epicotyls”
Claim · model-written summary
The photoreversible effect in pea mitochondrial fractions lasted only a few minutes after irradiation.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- photoreversible effecte0
- Value
- a few minutese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The photoreversible effect was seen only for a few minutes after the irradiation”
e1“pea (Pisum sativum cv. Alaska) epicotyls”
Claim · model-written summary
Hypotonic treatment cancelled the pea mitochondrial photoreversible response.
- Taxon
- peaLathyrus oleraceuse2
- Treatment
- hypotonic treatmente0
- Outcome
- photoreversible effecte1
- Direction
- cancellede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was cancelled by hypotonic treatment or addition of Triton X-100”
e1“The photoreversible effect”
e2“pea (Pisum sativum cv. Alaska) epicotyls”
Claim · model-written summary
Triton X-100 addition cancelled the pea mitochondrial photoreversible response.
- Taxon
- peaLathyrus oleraceuse2
- Treatment
- addition of Triton X-100e0
- Outcome
- photoreversible effecte1
- Direction
- cancellede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was cancelled by hypotonic treatment or addition of Triton X-100”
e1“The photoreversible effect”
e2“pea (Pisum sativum cv. Alaska) epicotyls”
Claim · model-written summary
The pea microsome fraction showed a similar but lesser photoreversible effect.
- Taxon
- peaLathyrus oleraceuse0
- Outcome
- effecte0
- Direction
- lessere0
- Comparator
- pea microsome fractione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A similar but lesser effect was observed in the pea microsome fraction”
e1“photoreversible effect”
Claim · model-written summary
A pea supernatant fraction showed no photoreversible response.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- photoreversible responsee0
- Direction
- noe0
- Qualifier
- supernatant fraction resulting from centrifugation at 10(5)g for 30 minutese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no photoreversible response was seen with a supernatant fraction resulting from centrifugation at 10(5)g for 30 minutes”
e1“pea microsome fraction”
The same questions in other papers
light · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
25 papers in the same direction as this paper · 20 in the opposite direction · 3 reporting no difference from an alternative
- negative
- no effect
- negative
45 papers more in this group
See all 49 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.