Abstract
Legume seeds and peanuts, in particular, are an inexpensive source of plant proteins and edible oil. A comprehensive understanding of seed metabolism and the effects of water-deficit stress on the incorporation of the main storage reserves in seeds, such as proteins, fatty acids, starch, and secondary metabolites, will enhance our ability to improve seed quality and yield through molecular breeding programs. In the present study, we employed a label-free quantitative proteomics approach to study the functional proteins altered in the midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions. We created a pod-specific proteome database and identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds. Mapping of these differential proteins revealed three candidate biological pathways (glycolysis, sucrose and starch metabolism, and fatty acid metabolism) that were significantly altered due to water-deficit stress. Differential accumulation of proteins from these pathways provides insight into the molecular mechanisms underlying the observed physiological changes, which include reductions in pod yield and biomass, reduced germination, reduced vigor, decreased seed membrane integrity, increase in storage proteins, and decreased total fatty acid content. Some of the proteins encoding rate limiting enzymes of biosynthetic pathways could be utilized by breeders to improve peanut seed production during water-deficit conditions in the field. The data have been deposited to the ProteomeXchange with identifier PXD000308.
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Claims 9
Claim · model-written summary
Label-free proteomics identified 93 differentially expressed proteins between well-watered and drought-stressed midmature peanut seeds.
- Taxon
- peanut
Arachis hypogaeae0 - Organ / stage
- midmature (65-70 days postanthesis) peanut seede0
- Treatment
- water-deficit stresse0
- Outcome
- differentially expressed proteinse1
- Value
- 93e1
- Comparator
- well-waterede1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions”
e1“identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds”
Claim · model-written summary
Three candidate pathways (glycolysis, sucrose and starch metabolism, and fatty acid metabolism) were significantly altered in peanut seed by water-deficit stress.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- water-deficit stresse2
- Outcome
- three candidate biological pathways (glycolysis, sucrose and starch metabolism, and fatty acid metabolism)e2
- Comparator
- well-waterede1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions”
e1“identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds”
e2“Mapping of these differential proteins revealed three candidate biological pathways (glycolysis, sucrose and starch metabolism, and fatty acid metabolism) that were significantly altered due to water-deficit stress”
Claim · model-written summary
Water-deficit stress reduced pod yield and biomass of peanut.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- water-deficit stresse0
- Outcome
- pod yield and biomasse3
- Direction
- reductionse3
- Comparator
- well-waterede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions”
e1“identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds”
e3“which include reductions in pod yield and biomass, reduced germination, reduced vigor, decreased seed membrane integrity, increase in storage proteins, and decreased total fatty acid content”
Claim · model-written summary
Water-deficit stress reduced germination of peanut seed.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- water-deficit stresse0
- Outcome
- germinatione3
- Direction
- reducede3
- Comparator
- well-waterede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions”
e1“identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds”
e3“which include reductions in pod yield and biomass, reduced germination, reduced vigor, decreased seed membrane integrity, increase in storage proteins, and decreased total fatty acid content”
Claim · model-written summary
Water-deficit stress reduced vigor of peanut seed.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- water-deficit stresse0
- Outcome
- vigore3
- Direction
- reducede3
- Comparator
- well-waterede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions”
e1“identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds”
e3“which include reductions in pod yield and biomass, reduced germination, reduced vigor, decreased seed membrane integrity, increase in storage proteins, and decreased total fatty acid content”
Claim · model-written summary
Water-deficit stress decreased seed membrane integrity of peanut seed.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- water-deficit stresse0
- Outcome
- seed membrane integritye3
- Direction
- decreasede3
- Comparator
- well-waterede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions”
e1“identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds”
e3“which include reductions in pod yield and biomass, reduced germination, reduced vigor, decreased seed membrane integrity, increase in storage proteins, and decreased total fatty acid content”
Claim · model-written summary
Water-deficit stress increased storage proteins in peanut seed.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- water-deficit stresse0
- Outcome
- storage proteinse3
- Direction
- increasee3
- Comparator
- well-waterede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions”
e1“identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds”
e3“which include reductions in pod yield and biomass, reduced germination, reduced vigor, decreased seed membrane integrity, increase in storage proteins, and decreased total fatty acid content”
Claim · model-written summary
Water-deficit stress decreased total fatty acid content of peanut seed.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- water-deficit stresse0
- Outcome
- total fatty acid contente3
- Direction
- decreasede3
- Comparator
- well-waterede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“midmature (65-70 days postanthesis) peanut seed grown under water-deficit stress conditions”
e1“identified 93 nonredundant, statistically significant, and differentially expressed proteins between well-watered and drought-stressed seeds”
e3“which include reductions in pod yield and biomass, reduced germination, reduced vigor, decreased seed membrane integrity, increase in storage proteins, and decreased total fatty acid content”
Claim · model-written summary
Proteins encoding rate-limiting enzymes of biosynthetic pathways could be used by breeders to improve peanut seed production under water-deficit conditions in the field.
- Taxon
- peanutArachis hypogaeae4
- Treatment
- water-deficit conditionse4
- Outcome
- peanut seed productione4
- Setting
- in the fielde4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Some of the proteins encoding rate limiting enzymes of biosynthetic pathways could be utilized by breeders to improve peanut seed production during water-deficit conditions in the field”
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See all 79 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.