Abstract
Callus and suspension cultures of Trifolium repens (white clover) were established from seedlings. Protoplasts, isolated enzymatically from cell aggregates of suspension cultures, produced callus which was capable of in vitro differentiation into complete plants. Regenerated plants were nodulated if inoculated with Rhizobium trifolii Karyotypic stability (2n = 4x = 32) was maintained for 2 yr of callus/suspension culture and through organogenesis Suspension cultures were treated enzymatically to give "single-cell" populations capable of high plating efficiencies in soft-agar media.
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Claims 5
Claim · model-written summary
Callus and suspension cultures were established from white clover seedlings.
- Taxon
- Trifolium repens
Trifolium repense0 - Organ / stage
- seedlingse0
- Outcome
- Callus and suspension culturese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus and suspension cultures of Trifolium repens (white clover) were established from seedlings”
Claim · model-written summary
Protoplast derived callus differentiated into complete plants.
- Taxon
- Trifolium repensTrifolium repense1
- Outcome
- in vitro differentiation into complete plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protoplasts, isolated enzymatically from cell aggregates of suspension cultures, produced callus which was capable of in vitro differentiation into complete plants”
e1“Trifolium repens (white clover)”
Claim · model-written summary
Regenerated plants nodulated after Rhizobium trifolii inoculation.
- Taxon
- Trifolium repensTrifolium repense1
- Treatment
- inoculated with Rhizobium trifoliie0
- Interactor
- Rhizobium trifolii
Rhizobium leguminosarume0symbiont - Outcome
- Regenerated plants were nodulatede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plants were nodulated if inoculated with Rhizobium trifolii”
e1“Trifolium repens (white clover)”
Claim · model-written summary
Karyotype stayed stable for two years in culture and through organogenesis.
- Taxon
- Trifolium repensTrifolium repense1
- Outcome
- Karyotypic stabilitye0
- Value
- 2 yre0
- Qualifier
- 2n = 4x = 32e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Karyotypic stability (2n = 4x = 32) was maintained for 2 yr of callus/suspension culture and through organogenesis”
e1“Trifolium repens (white clover)”
Claim · model-written summary
Enzymatic treatment yielded single cell populations with high plating efficiencies.
- Taxon
- Trifolium repensTrifolium repense1
- Outcome
- high plating efficienciese0
Not reportedComparatorDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suspension cultures were treated enzymatically to give "single-cell" populations capable of high plating efficiencies in soft-agar media”
e1“Trifolium repens (white clover)”
The same questions in other papers
Rhizobium leguminosarum · symbiosis · Trifolium repens (white clover)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
inoculation · symbiosis · Trifolium repens (white clover)Positive and negative results
8 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.