Abstract
Transfer of an IncP plasmid carrying the Rhizobium meliloti nodFE, nodG, and nodH genes to Rhizobium trifolii enabled R. trifolii to nodulate alfalfa (Medicago sativa), the normal host of R. meliloti. Using transposon Tn5-linked mutations and in vitro-constructed deletions of the R. meliloti nodFE, nodG, and nodH genes, we showed that R. meliloti nodH was required for R. trifolii to elicit both root hair curling and nodule initiation on alfalfa and that nodH, nodFE, and nodG were required for R. trifolii to elicit infection threads in alfalfa root hairs. Interestingly, the transfer of the R. meliloti nodFE, nodG, and nodH genes to R. trifolii prevented R. trifolii from infecting and nodulating its normal host, white clover (Trifolium repens). Experiments with the mutated R. meliloti nodH, nodF, nodE, and nodG genes demonstrated that nodH, nodF, nodE, and possibly nodG have an additive effect in blocking infection and nodulation of clover.
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Claims 4
Claim · model-written summary
R. meliloti nodH is required for R. trifolii to elicit root hair curling and nodule initiation on alfalfa.
- Taxon
- alfalfa
Medicago sativae1 - Interactor
- R. trifolii
Rhizobium leguminosarume1symbiont - Gene
- R. meliloti nodHe1
- Outcome
- root hair curling and nodule initiatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“R. meliloti nodH was required for R. trifolii to elicit both root hair curling and nodule initiation on alfalfa”
Claim · model-written summary
R. meliloti nodH, nodFE and nodG are required for R. trifolii to elicit infection threads in alfalfa root hairs.
- Taxon
- alfalfaMedicago sativae2
- Interactor
- R. trifoliiRhizobium leguminosarume2symbiont
- Gene
- nodH, nodFE, and nodGe2
- Outcome
- infection threadse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“nodH, nodFE, and nodG were required for R. trifolii to elicit infection threads in alfalfa root hairs”
Claim · model-written summary
Transfer of R. meliloti nodFE, nodG and nodH genes to R. trifolii prevented R. trifolii from infecting and nodulating white clover.
- Taxon
- white clover
Trifolium repense3 - Treatment
- the transfer of the R. meliloti nodFE, nodG, and nodH genes to R. trifoliie3
- Interactor
- R. trifoliiRhizobium leguminosarume3symbiont
- Outcome
- infecting and nodulatinge3
- Direction
- preventede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“the transfer of the R. meliloti nodFE, nodG, and nodH genes to R. trifolii prevented R. trifolii from infecting and nodulating its normal host, white clover (Trifolium repens)”
Claim · model-written summary
nodH, nodF, nodE and possibly nodG have an additive effect in blocking infection and nodulation of clover.
- Gene
- nodH, nodF, nodE, and possibly nodGe4
- Outcome
- blocking infection and nodulatione4
- Qualifier
- additive effecte4
TrifoliumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Experiments with the mutated R. meliloti nodH, nodF, nodE, and nodG genes demonstrated that nodH, nodF, nodE, and possibly nodG have an additive effect in blocking infection and nodulation of clover”
The same questions in other papers
Rhizobium leguminosarum · symbiosis · Trifolium repens (white clover)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.