Abstract
The incorporation of adenosine-8-(14)C into adenosine cyclic 3':5'-monophosphate in coleoptile-first leaf segments of Avena sativa L. was investigated. Homogenates of segments incubated in adenosine-8-(14)C for either 4 or 10 hours were partially purified by thin layer chromatography followed by paper electrophoresis. A radioactive fraction, less than 0.06% of the (14)C present in the original homogenate, migrated as adenosine cyclic 3':5'-monophosphate during electrophoresis. Upon treatment with cyclic nucleotide phosphodiesterase, however, less than 10% of this radioactive fraction appeared as 5'-AMP. Deamination with NaNO(2) as well as further chromatographical purification also suggested that only a small fraction of the (14)C in the partially purified samples could be in adenosine cyclic 3':5'-monophosphate. The data suggest that levels of this nucleotide can probably be no greater than 7 to 11 picomoles per gram of fresh weight in oat coleoptiles. Treatment of such coleoptiles with physiologically active concentrations of indoleacetic acid, furthermore, had no significant effect on the (14)C radioactivity in marker adenosine cyclic 3':5'-monophosphate-containing fractions at any stage of purification during several experiments.In a single experiment, no labeled guanosine cyclic 3':5'-monophosphate could be detected in oat coleoptile-first leaf segments incubated in guanosine-8-(14)C either with or without indoleacetic acid. These results do not support the hypothesis that a cyclic nucleotide mediates the action of indoleacetic acid on oat coleoptile extension.
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Claims 7
Claim · model-written summary
A radioactive fraction amounting to less than 0.06% of the (14)C in the original homogenate migrated as cyclic AMP during electrophoresis.
- Taxon
- Oat
Avena sativat0 - Outcome
- migrated as adenosine cyclic 3':5'-monophosphate during electrophoresise0
- Value
- less than 0.06%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat”
e0“A radioactive fraction, less than 0.06% of the (14)C present in the original homogenate, migrated as adenosine cyclic 3':5'-monophosphate during electrophoresis”
Claim · model-written summary
After treatment with cyclic nucleotide phosphodiesterase, less than 10% of this radioactive fraction appeared as 5'-AMP.
- Taxon
- OatAvena sativat0
- Treatment
- cyclic nucleotide phosphodiesterasee1
- Outcome
- appeared as 5'-AMPe1
- Value
- less than 10%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat”
e1“Upon treatment with cyclic nucleotide phosphodiesterase, however, less than 10% of this radioactive fraction appeared as 5'-AMP”
Claim · model-written summary
Deamination with NaNO2 and further chromatographic purification also suggested that only a small fraction of the (14)C in the partially purified samples could be cyclic AMP.
- Taxon
- OatAvena sativat0
- Treatment
- Deamination with NaNO(2)e2
- Outcome
- only a small fraction of the (14)C in the partially purified samples could be in adenosine cyclic 3':5'-monophosphatee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat”
e2“Deamination with NaNO(2) as well as further chromatographical purification also suggested that only a small fraction of the (14)C in the partially purified samples could be in adenosine cyclic 3':5'-monophosphate”
Claim · model-written summary
Levels of cyclic AMP in oat coleoptiles can probably be no greater than 7 to 11 picomoles per gram of fresh weight.
- Taxon
- oatAvena sativae3
- Organ / stage
- coleoptilese3
- Outcome
- levels of this nucleotidee3
- Value
- 7 to 11 picomoles per gram of fresh weighte3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat”
e3“The data suggest that levels of this nucleotide can probably be no greater than 7 to 11 picomoles per gram of fresh weight in oat coleoptiles”
Claim · model-written summary
Treatment of oat coleoptiles with physiologically active concentrations of indoleacetic acid had no significant effect on the (14)C radioactivity in cyclic AMP-containing fractions at any stage of purification.
- Taxon
- OatAvena sativat0
- Organ / stage
- coleoptilese4
- Treatment
- physiologically active concentrations of indoleacetic acide4
- Outcome
- (14)C radioactivity in marker adenosine cyclic 3':5'-monophosphate-containing fractionse4
- Direction
- no significant effecte4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat”
e4“Treatment of such coleoptiles with physiologically active concentrations of indoleacetic acid, furthermore, had no significant effect on the (14)C radioactivity in marker adenosine cyclic 3':5'-monophosphate-containing fractions at any stage of purification”
Claim · model-written summary
In a single experiment, no labeled guanosine cyclic 3':5'-monophosphate could be detected in oat coleoptile-first leaf segments incubated in guanosine-8-(14)C with or without indoleacetic acid.
- Taxon
- oatAvena sativae5
- Organ / stage
- coleoptile-first leaf segmentse5
- Treatment
- indoleacetic acide5
- Outcome
- no labeled guanosine cyclic 3':5'-monophosphate could be detectede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“no labeled guanosine cyclic 3':5'-monophosphate could be detected in oat coleoptile-first leaf segments incubated in guanosine-8-(14)C either with or without indoleacetic acid”
Claim · model-written summary
The results do not support the hypothesis that a cyclic nucleotide mediates the action of indoleacetic acid on oat coleoptile extension.
- Taxon
- oatAvena sativae6
- Treatment
- indoleacetic acide6
- Compound
- cyclic nucleotidee6
- Outcome
- oat coleoptile extensione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These results do not support the hypothesis that a cyclic nucleotide mediates the action of indoleacetic acid on oat coleoptile extension”
The same questions in other papers
indole-3-acetic acid · molecular: metabolites · Avena sativa (oats)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.