Abstract
Guard cell protoplasts (GCP) from leaves of pea (Pisum sativum) were capable of reducing/oxidizing the membrane impermeable electron carriers, ferricyanide/NADH. The redox activity of GCP required the presence of both ferricyanide and NADH, although some ferricyanide reduction occurred even in the absence of NADH. The GCP preferred NADH to NADPH during ferricyanide reduction and the reduction was slow with DCPIP or cytochrome c. A stoichiometry of about 2 existed between moles of ferricyanide reduced and NADH oxidized by GCP. The redox activities of GCP were several times greater than those of mesophyll protoplasts from pea leaves. The ferricyanide reduction or NADH oxidation by GCP was unaffected by abscisic acid or sodium orthovanadate and fusicoccin indicating the non-involvement of plasma membrane ATPase in these redox reactions.The redox activities were markedly inhibited by chloroquine or 8-hydroxyquinoline. The findings are discussed in relation to the possible regulatory role of a guard cell plasma membrane redox system in stomatal function.
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Claims 8
Claim · model-written summary
Pea guard cell protoplasts reduced ferricyanide and oxidized NADH.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- Guard cell protoplastse0
- Outcome
- reducing/oxidizing the membrane impermeable electron carriers, ferricyanide/NADHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Guard cell protoplasts (GCP) from leaves of pea (Pisum sativum) were capable of reducing/oxidizing the membrane impermeable electron carriers, ferricyanide/NADH.”
Claim · model-written summary
GCP redox activity required ferricyanide and NADH, though some ferricyanide reduction occurred without NADH.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- redox activity of GCPe0
- Qualifier
- presence of both ferricyanide and NADHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The redox activity of GCP required the presence of both ferricyanide and NADH, although some ferricyanide reduction occurred even in the absence of NADH.”
t0“Pea”
Claim · model-written summary
GCP preferred NADH over NADPH in ferricyanide reduction.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- ferricyanide reductione0
- Direction
- preferrede0
- Comparator
- NADPHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GCP preferred NADH to NADPH during ferricyanide reduction”
t0“Pea”
Claim · model-written summary
Ferricyanide reduction was slow with DCPIP or cytochrome c.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- reductione0
- Direction
- slowe0
- Qualifier
- DCPIP or cytochrome ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the reduction was slow with DCPIP or cytochrome c”
t0“Pea”
Claim · model-written summary
The molar ratio of ferricyanide reduced to NADH oxidized by GCP was about 2.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- stoichiometrye0
- Value
- about 2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A stoichiometry of about 2 existed between moles of ferricyanide reduced and NADH oxidized by GCP.”
t0“Pea”
Claim · model-written summary
GCP redox activities were several times greater than those of mesophyll protoplasts.
- Taxon
- peaLathyrus oleraceuse0
- Outcome
- redox activitiese0
- Direction
- greatere0
- Value
- several timese0
- Comparator
- mesophyll protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The redox activities of GCP were several times greater than those of mesophyll protoplasts from pea leaves.”
Claim · model-written summary
Abscisic acid, sodium orthovanadate and fusicoccin did not affect GCP ferricyanide reduction or NADH oxidation.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- abscisic acid or sodium orthovanadate and fusicoccine0
- Outcome
- ferricyanide reduction or NADH oxidatione0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ferricyanide reduction or NADH oxidation by GCP was unaffected by abscisic acid or sodium orthovanadate and fusicoccin”
t0“Pea”
Claim · model-written summary
Chloroquine or 8-hydroxyquinoline markedly inhibited GCP redox activities.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- chloroquine or 8-hydroxyquinolinee0
- Outcome
- redox activitiese0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The redox activities were markedly inhibited by chloroquine or 8-hydroxyquinoline.”
t0“Pea”
The same questions in other papers
fusicoccin · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.